Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
p663-UBC-miniTurbo-V5-RPS6KL1_IDG-K Resource Report Resource Website |
RRID:Addgene_170605 | miniTurbo-V5-RPS6KL1 | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:09 | 0 | ||
|
pJCX4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170756 | Kanamycin | PMID:34478714 | Please visit https://www.biorxiv.org/content/10.1101/2021.04.05.438513v2 for BioRxiv preprint | Backbone Size:5409; Vector Backbone:pJCX4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:10 | 1 | |||
|
pTetQCas-8+IS186 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170636 | VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array) | Vibrio cholera | Streptomycin | PMID:34152213 | Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2026-08-15 01:10:09 | 1 | ||
|
pAraQCas-BsaI Resource Report Resource Website |
RRID:Addgene_170633 | VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI) | Vibrio cholera | Streptomycin | PMID:34152213 | Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2026-08-15 01:10:09 | 0 | ||
|
pRhaQCas-BsaI Resource Report Resource Website |
RRID:Addgene_170631 | VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI) | Vibrio cholera | Streptomycin | PMID:34152213 | Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2026-08-15 01:10:10 | 0 | ||
|
pSUMO chicken-cPLA2-C2-domain Resource Report Resource Website |
RRID:Addgene_170753 | PLA2G4A-C2 domain | Gallus gallus | Kanamycin | PMID:31050338 | Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:10 | 0 | ||
|
pRE57I-GNAc Resource Report Resource Website |
RRID:Addgene_170637 | Mini-Tn6677, GNAc cassette, lacI | Saccharomyces cerevisiae | Ampicillin | PMID:34152213 | Vector Backbone:pUC57mini; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
|
pLIC-lyn11-myc-LOV2GIVe (D) Resource Report Resource Website |
RRID:Addgene_170625 | Lyn11-myc-LOV2GIVe (D) | Synthetic | Ampicillin | PMID:32936073 | Backbone Size:6000; Vector Backbone:pLIC-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
|
pTetQCas-BsaI Resource Report Resource Website |
RRID:Addgene_170629 | VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI) | Vibrio cholera | Streptomycin | PMID:34152213 | Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2026-08-15 01:10:09 | 0 | ||
|
ZE31 Prickle-1-pCS2P+ Resource Report Resource Website |
RRID:Addgene_17072 | Prickle-1 | Danio rerio | Ampicillin | PMID:12699626 | Description: Zebrafish prickle-1 (full-length ORF), cloned into the BamH1 and EcoR1 sites of CS2P+. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003). | Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | |
|
ZE22 vox-CS (ZV100) Resource Report Resource Website |
RRID:Addgene_17064 | vox-2 | Danio rerio | Ampicillin | Zvox-2 coding region amplified by PCR and inserted into CS2+ (first cloned into SK as zV93). For RNA: cut with Asp718 and transcribe with SP6. Plasmid History: Species of Sequence Origin: Zebrafish. Constructed by: David Kimelman, 6/28/97 XIX p. 67. | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
|
ZE26 Stbm-N-6myc-pCS2+ Resource Report Resource Website |
RRID:Addgene_17068 | Stbm | Danio rerio | Ampicillin | PMID:11780127 | 6-c-myc epitopes in frame at the amino terminus of wild type zebrafish strabismus; EcoRI-Xba1 insert. For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002. | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | |
|
pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR Resource Report Resource Website |
RRID:Addgene_170810 | Ampicillin | PMID:34133940 | 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint. | Vector Backbone:lentiCRISPR v2 (Addgene #52961); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | NA | 2026-08-15 01:10:10 | 0 | ||
|
pFUGW-FIRE-pHLy Resource Report Resource Website 1+ mentions |
RRID:Addgene_170774 | LAMP1 | Homo sapiens | Ampicillin | PMID:34102054 | Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318 | Backbone Marker:Addgene (Plasmid #14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 84 nucleotide Signal Sequence switched to the beginning of the construct | 2026-08-15 01:10:11 | 5 |
|
pLJM1-FIRE-pHLy Resource Report Resource Website 1+ mentions |
RRID:Addgene_170775 | LAMP1 | Homo sapiens | Ampicillin | PMID:34102054 | Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318 | Backbone Marker:Addgene Plasmid #19319; Backbone Size:8083; Vector Backbone:pLJM1-EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 84 nucleotide Signal Sequence switched to the beginning of the construct | 2026-08-15 01:10:10 | 4 |
|
lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC Resource Report Resource Website |
RRID:Addgene_170772 | lacI | Synthetic | Ampicillin | PMID:35255734 | This plasmid was designed to test Golden Gate assembly protocols | Backbone Marker:pTwist; Backbone Size:3; Vector Backbone:pUC; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | |
|
AICSDP-116:PCNA-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_170818 | PCNA Homology Arms with mEGFP-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human PCNA in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GEGQGQGQGPGRGYAYRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used, and WTC-11 specific homozygous SNP(s) | 2026-08-15 01:10:11 | 1 | |
|
pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR Resource Report Resource Website |
RRID:Addgene_170813 | NLS-mScarlet-I-P2A-BlastR | Synthetic; B. coriaceae | Ampicillin | PMID:34133940 | 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint. | Vector Backbone:pW212 (Addgene #170810); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | NA | 2026-08-15 01:10:11 | 0 |
|
lacI-Ptac-lacO-IMBB4-pUC Resource Report Resource Website |
RRID:Addgene_170770 | lacI | Synthetic | Ampicillin | PMID:35255734 | This plasmid was designed to test 5RM assembly protocols | Backbone Marker:GenScript; Backbone Size:1835; Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:11 | 0 | |
|
BsaI-tagRFP-BsaI-pUC-kan Resource Report Resource Website |
RRID:Addgene_170771 | tagRFP | Synthetic | Kanamycin | PMID:35255734 | This plasmid was designed to test Golden Gate assembly protocols | Backbone Marker:GenScript; Backbone Size:2579; Vector Backbone:pUC; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:10 | 0 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
