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Species: Synthetic
Genetic Insert: Putidaredoxin
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5333; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The PdX gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_85084 Copy
Genetic Insert: EGFP-FKBP(M)x4
Vector Backbone Description: Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27738551
Proper citation: RRID:Addgene_85004 Copy
Species: Homo sapiens
Genetic Insert: hTERT
Vector Backbone Description: Backbone Size:9211; Vector Backbone:pLV-EF1a-IRES-Hygro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27842057
Comments: The 3rd generation packaging system (pMDLg/pRRE, pRSV-rev, and pVSVG) was used to create virus from this lentiviral transfer vector.
Proper citation: RRID:Addgene_85140 Copy
Genetic Insert: LuxR
Vector Backbone Description: Vector Backbone:pBjk2807; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27172092
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_85142 Copy
Genetic Insert: Ftractin-mRuby3-P2A-mTurquoise-MLC
Vector Backbone Description: Backbone Size:8614; Vector Backbone:pLV-EF1a-IRES-Blast; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27842057
Comments: Stoichiometric expression of Ftractin-mRuby3 and mTurquiose-MLC is achieved through insertion of a ribosomal skipping sequence (P2A) between the two reporter sequences.
Proper citation: RRID:Addgene_85146 Copy
Vector Backbone Description: Backbone Size:9211; Vector Backbone:pLV-EF1a-IRES-Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27842057
Comments: - Efficient selection of stably transduced cells due to IRES-driven selectable marker.
- Suitable cloning sites are BamHI/EcoRI, BamHI/NotI.
- Compatible with 2nd and 3rd generation lentiviral packaging systems.
Proper citation: RRID:Addgene_85139 Copy
Species: Mus musculus
Genetic Insert: HOXA9
Vector Backbone Description: Backbone Size:0; Vector Backbone:MIG (MSCV IRES GFP); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343407
Comments: Note: Addgene verified sequence confirms that the FLAG sequence is DYKDDDDK.
Proper citation: RRID:Addgene_8515 Copy
Vector Backbone Description: Backbone Size:8785; Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27842057
Comments: - Efficient selection of stably transduced cells due to IRES-driven selectable marker.
- Suitable cloning sites are BamHI/EcoRI, BamHI/NotI.
- Compatible with 2nd and 3rd generation lentiviral packaging systems.
Proper citation: RRID:Addgene_85132 Copy
Species: Rattus norvegicus
Genetic Insert: Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
Vector Backbone Description: Backbone Size:9234; Vector Backbone:CSII-EF; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27842057
Proper citation: RRID:Addgene_85131 Copy
Vector Backbone Description: Backbone Size:8614; Vector Backbone:pLV-EF1a-IRES-Blast; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27842057
Comments: - Efficient selection of stably transduced cells due to IRES-driven selectable marker.
- Suitable cloning sites are BamHI/EcoRI, BamHI/NotI.
- Compatible with 2nd and 3rd generation lentiviral packaging systems.
Proper citation: RRID:Addgene_85133 Copy
Species: Homo sapiens
Genetic Insert: hAur-A
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15536123
Comments: See author's map. For production of retrovirus expressing human Aur-A wild-type. (Ruderman RC232)
Proper citation: RRID:Addgene_8510 Copy
Species: E. coli strain RY13
Genetic Insert: EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2155; Vector Backbone:pSB1A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25524101
Comments: AmilCP is fused to the EcoRI Methylase. RTX tag is fused to the EcoRI restriction enzyme.
The methylase will methylate the EcoRI cloning site, rendering the site nonfunctional.
Proper citation: RRID:Addgene_85165 Copy
Species: Homo sapiens
Genetic Insert: YTHDC1
Vector Backbone Description: Backbone Marker:Sellers Lab, Addgene plasmid 10792; Backbone Size:5506; Vector Backbone:pcDNA3 FLAG HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27602518
Proper citation: RRID:Addgene_85167 Copy
Species: E. coli strain RY13
Genetic Insert: EcoRI Methylase-AmilCP
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2155; Vector Backbone:pSB1A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25524101
Comments: The methylase will methylate the EcoRI cloning site, rendering the site nonfunctional.
Proper citation: RRID:Addgene_85166 Copy
Vector Backbone Description: Vector Backbone:pBBR1MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8529885
Comments: Vector is mobilisable via RP4/RK2 mating system, e.g. using E.coli strains S17 or SM10. Note that cloned genes are expressed from the lac promoter, but that this is not controllable with IPTG, due to absence of lacI repressor gene on the plasmid. (chromosomal lacI does not provide sufficient repression).
MCS: Has unique KpnI, ApaI, XhoI, HincII, SalI, ClaI, HindIII, EcoRV, EcoRI, SmaI, BamHI, SpeI, XbaI, SacII, SacI sites for cloning
Proper citation: RRID:Addgene_85168 Copy
Species: Mus musculus
Genetic Insert: CA-RIT-NFAT1
Vector Backbone Description: Backbone Marker:Anjana Rao lab; Vector Backbone:pRV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25680272
Comments: RIT mutations (R468, I469, and T535) selectively interfere with NFAT:AP-1 interaction
Proper citation: RRID:Addgene_85181 Copy
Species: Homo sapiens
Genetic Insert: Hsp27
Vector Backbone Description: Vector Backbone:pFLAG-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The pFLAG-CMV2-Hsp27 wild-type plasmid was obtained from Dr. Park and is described by Park KJ, Gaynor RB, Kwak YT. J Biol Chem 2003; 278:35272-35278. PMID:12829720
Proper citation: RRID:Addgene_85188 Copy
Species: Homo sapiens
Genetic Insert: human mature (delta25) MCAD
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25416781
Comments: Homo sapiens wt MCAD, delta 25, cloned into NdeI-NotI sites of pET28a, contains a N-terminal His-tag
Proper citation: RRID:Addgene_85112 Copy
Species: Homo sapiens
Genetic Insert: human mature (delta44) GCDH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25416781
Comments: Homo sapiens wt GCDH, delta 44, cloned into NdeI-NotI sites of pET28a, contains a N-terminal His-tag
Proper citation: RRID:Addgene_85113 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pSicoR_mCherry without PuroR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798611
Proper citation: RRID:Addgene_85412 Copy
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