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Species: S.pyogenes
Genetic Insert: humanised S.pyogenes Cas9
Vector Backbone Description: Backbone Marker:David Baltimore; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_85555 Copy
Species: Synthetic
Genetic Insert: KRAB-dCas9-IRES-GFP
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8812; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27708057
Proper citation: RRID:Addgene_85556 Copy
Species: Other
Genetic Insert: pRSV
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27246850
Comments: We replaced the pMecp2 promoter of SpCas9 (Addgene: 60957) with a RSV (Rous Sarcoma Virus) promoter, which was amplified from a lentiviral vector for shRNA-MDM2
Proper citation: RRID:Addgene_85450 Copy
Vector Backbone Description: Backbone Size:2499; Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27732583
Proper citation: RRID:Addgene_85527 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20082521
Proper citation: RRID:Addgene_85484 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBAD/Myc-His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20082521
Proper citation: RRID:Addgene_85482 Copy
Species: Homo sapiens
Genetic Insert: hTG2
Vector Backbone Description: Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23085087
Proper citation: RRID:Addgene_85470 Copy
Species: Homo sapiens
Genetic Insert: KCNH1
Vector Backbone Description: Vector Backbone:pSGEM; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12554641
Comments: pSGEM vector, 3118 bp, engineered by Michael Hollmann & Dorothea Gremmels, March 1994. pSGEM is based on the pGEMHE-MCS vector (3096 bp) engineered by M.Hollmann. pGEMHE-MCS itself is based on the pGEMHE vector (3022 bp) which in turn is derived from pGEM-3Z of Promega (2743 bp) See also Liman,E.R., Tytgat,J. and Hess,P. Neuron (1992) Vol.9, pp.861-871
Proper citation: RRID:Addgene_85704 Copy
Species: Homo sapiens
Genetic Insert: KCNH5
Vector Backbone Description: Vector Backbone:pTracetCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17671204
Proper citation: RRID:Addgene_85706 Copy
Vector Backbone Description: Vector Backbone:pHKO9; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27984734
Proper citation: RRID:Addgene_85707 Copy
Vector Backbone Description: Backbone Marker:Eric Lander and David Sabatini (Addgene #71409); Backbone Size:7000; Vector Backbone:pLenti-sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27708057
Proper citation: RRID:Addgene_85681 Copy
Species: Brackiella oedipodis
Genetic Insert: Type II-C anti-CRISPR AcrIIC1Boe
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27984730
Proper citation: RRID:Addgene_85678 Copy
Species: Neisseria meningitidis
Genetic Insert: Type II-C anti-CRISPR AcrIIC1Nme
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27984730
Proper citation: RRID:Addgene_85679 Copy
Species: Homo sapiens
Genetic Insert: 10XHis-GST-HRV-Her2-dL5-Her2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5387; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
Proper citation: RRID:Addgene_85624 Copy
Species: Drosophila melanogaster
Genetic Insert: yki
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18256197
Proper citation: RRID:Addgene_85622 Copy
Genetic Insert: Prab-3-GAL4-SK(DBD)-VP64
Vector Backbone Description: Backbone Marker:Fire Lab C. elegans Vector Kit; Backbone Size:2778; Vector Backbone:pPD117.01; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27992408
Proper citation: RRID:Addgene_85583 Copy
Vector Backbone Description: Backbone Size:12684; Vector Backbone:pRSF1010; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28000776
Comments: FnCpf1 from Francisella novicida
Proper citation: RRID:Addgene_85581 Copy
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2380; Vector Backbone:pMA-T; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27465215
Proper citation: RRID:Addgene_85586 Copy
Species: Caenorhabditis elegans
Genetic Insert: 15xUAS-delta pes-10-GFP
Vector Backbone Description: Backbone Marker:Fire Lab C. elegans Vector Kit; Backbone Size:2778; Vector Backbone:pPD117.01; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27992408
Proper citation: RRID:Addgene_85584 Copy
Species: S. pyogenes
Genetic Insert: cas9-tracrRNA
Vector Backbone Description: Backbone Size:6015; Vector Backbone:modified pAX01 (ColE1); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27260361
Proper citation: RRID:Addgene_85613 Copy
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