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Species: Synthetic
Genetic Insert: Barcode72
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27617390
Proper citation: RRID:Addgene_83749 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_83884 Copy
Species: E. coli
Genetic Insert: Gro EL/ES
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23220215
Proper citation: RRID:Addgene_83923 Copy
Species: Homo sapiens
Genetic Insert: glyceraldehyde-3-phosphate dehydrogenase
Vector Backbone Description: Backbone Size:5647; Vector Backbone:pET30-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27110680
Proper citation: RRID:Addgene_83910 Copy
Species: Homo sapiens
Genetic Insert: mKO2-SLBP(18-126)
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pLL3.7m; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798610
Proper citation: RRID:Addgene_83914 Copy
Species: Synthetic
Genetic Insert: Clover-Geminin(1-110)
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pLL3.7m; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798610
Proper citation: RRID:Addgene_83915 Copy
Species: Homo sapiens
Genetic Insert: Doublecortin isoform b
Vector Backbone Description: Backbone Marker:Peränen J., Rikkonen M.,Hyvönen M., Kääriäinen L. (1996) T7 vectors with modified T7lac promoter for expression of proteins in E; Vector Backbone:pHAT2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22727374
Proper citation: RRID:Addgene_83918 Copy
Genetic Insert: DsRed-Express2
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369033
Comments: Digest with BsmBI to remove ~1.9 kb stuffer for cloning in protospacer sequences.
Proper citation: RRID:Addgene_83919 Copy
Genetic Insert: floxed STOP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5200; Vector Backbone:pGL3 Control; Vector Types:Mammalian Expression, Cre/Lox, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11410679
Comments: See author's map.
Proper citation: RRID:Addgene_8390 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Dicarlo et al Nucleic Acids Res; Vector Backbone:p414-TEF1p-Cas9-CYC1t; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34150504
Proper citation: RRID:Addgene_83946 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798629
Proper citation: RRID:Addgene_83895 Copy
Genetic Insert: hM3Dq
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798629
Proper citation: RRID:Addgene_83897 Copy
Genetic Insert: ChR2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798629
Proper citation: RRID:Addgene_83898 Copy
Species: S. Pyogenes
Genetic Insert: humanized dead Cas9 KRAB
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369033
Proper citation: RRID:Addgene_83890 Copy
Species: Other
Genetic Insert: ERT2-spCas9-ERT2
Vector Backbone Description: Backbone Marker:Sytem Biosciences; Vector Backbone:Piggybac Transposon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that mutation R1058W in Cas9 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
The plasmid expresses a ERT2-SpCas9-ERT2 tamoxifen inducible Cas9 driven by the EF-1a promoter. T2A linked puromucin enables stable selection of the piggybac transposon. CRISPR gRNAS can be cloned into the vector at the BbsI cloning sites.
Proper citation: RRID:Addgene_83961 Copy
Species: Homo sapiens
Genetic Insert: fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11675495
Proper citation: RRID:Addgene_83957 Copy
Species: Homo sapiens
Genetic Insert: Phosphoglycerate dehydrogenase
Vector Backbone Description: Backbone Size:8010; Vector Backbone:pLJM5; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27110680
Proper citation: RRID:Addgene_83901 Copy
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798629
Proper citation: RRID:Addgene_83900 Copy
Species: Homo sapiens
Genetic Insert: adeno-associated virus integration site 1
Vector Backbone Description: Backbone Size:11565; Vector Backbone:pLentiCRISPRv1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27110680
Proper citation: RRID:Addgene_83906 Copy
Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Size:8482; Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27641305
Comments: The construct FUW-tetO-EGFP was obtained from FUW-tetO-hOct4 (Addgene #20726) by replacing hOct4 with the EGFP-ires-puro cassette from PL-SIN-EOS-C(3+)-EiP (Addgene #21313).
Proper citation: RRID:Addgene_84041 Copy
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