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Species: Pyrearinus termitilluminans (E-Luc) and Luciola curciata (Red-F)
Genetic Insert: E-Luc
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2925; Vector Backbone:pDGB1_omega1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34520169
Comments: Encodes both E-Luc and Red-F transcriptional units. Insert can be excised by BsaI digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170906 Copy
Species: Homo sapiens
Genetic Insert: Synaptotagmin-like 5
Vector Backbone Description: Backbone Marker:Clonetech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34172739
Proper citation: RRID:Addgene_170868 Copy
Species: Synthetic
Genetic Insert: 14x-His-bdSUMO-muGFP-HiBiT
Vector Backbone Description: Backbone Size:4694; Vector Backbone:pET His6 MBP TEV LIC cloning vector (2M-T); Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34140497
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.08.20.258350v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170989 Copy
Species: Pyrearinus termitilluminans (E-Luc) and Luciola curciata (Red-F)
Genetic Insert: E-Luc
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2925; Vector Backbone:pDGB1_omega1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34520169
Comments: Encodes both E-Luc and Red-F transcriptional units. Insert can be excised by BsaI digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170899 Copy
Species: Pyrearinus termitilluminans (E-Luc) and Luciola curciata (Red-F)
Genetic Insert: E-Luc
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2925; Vector Backbone:pDGB1_omega1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34520169
Comments: Encodes both E-Luc and Red-F transcriptional units. Insert can be excised by BsaI digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170896 Copy
Species: Pyrearinus termitilluminans (E-Luc) and Luciola curciata (Red-F)
Genetic Insert: E-Luc
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2925; Vector Backbone:pDGB1_omega1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34520169
Comments: Encodes both E-Luc and Red-F transcriptional units. Insert can be excised by BsaI digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170893 Copy
Species: Pyrearinus termitilluminans (E-Luc) and Luciola curciata (Red-F)
Genetic Insert: E-Luc
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2925; Vector Backbone:pDGB1_omega1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34520169
Comments: Encodes both E-Luc and Red-F transcriptional units. Insert can be excised by BsaI digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170891 Copy
Species: Pyrearinus termitilluminans (E-Luc) and Luciola curciata (Red-F)
Genetic Insert: E-Luc
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2925; Vector Backbone:pDGB1_omega1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34520169
Comments: Encodes both E-Luc and Red-F transcriptional units. Insert can be excised by BsaI digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170890 Copy
Species: Luciola curciata
Genetic Insert: Red-F
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2594; Vector Backbone:pDGB1_alpha2R; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34520169
Comments: Red-F transcriptional unit in GoldenBraid pDGB1 alpha2R. Insert can be excised by Esp3I digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170888 Copy
Species: Mus musculus
Genetic Insert: sgAgo1
Vector Backbone Description: Backbone Marker:Feng Zhang Lab (Addgene# 48138); Backbone Size:9272; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35236760
Proper citation: RRID:Addgene_170921 Copy
Species: Luciola curciata
Genetic Insert: Red-F
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2595; Vector Backbone:pDGB1_alpha2; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34520169
Comments: Red-F transcriptional unit in GoldenBraid pDGB1 alpha2. Insert can be excised by Esp3I digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170887 Copy
Species: Mus musculus
Genetic Insert: sgAgo2
Vector Backbone Description: Backbone Marker:Feng Zhang Lab (Addgene# 48138); Backbone Size:9272; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35236760
Proper citation: RRID:Addgene_170920 Copy
Species: Agrobacterium tumefaciens
Genetic Insert: NOSt
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34520169
Comments: Insert can be excised by BsaI digestion . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170885 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtACT2t
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34520169
Comments: Insert can be excised by BsaI digestion . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170882 Copy
Species: Synthetic
Genetic Insert: pEstradiol_Inducible
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34520169
Comments: Insert can be excised by BsaI digestion . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv
Proper citation: RRID:Addgene_170880 Copy
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCS2+ (w/ GFP); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Linearize with Not1 or Asp718 and transcribe with SP6.
Notes for Use:
For making carboxy-terminal tagged GFP fusion protein in vector CS2+.
Made by: Jeff Miller.
Proper citation: RRID:Addgene_17097 Copy
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCS2+ (w/ GFP); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Linearize with Not1 or Asp718 and transcribe with SP6.
Notes for Use:
For making amino-terminal GFP fusion protein in vector CS2+.
Made by: Jeff Miller.
Proper citation: RRID:Addgene_17099 Copy
Species: S. japonia/camelidae/Staphylococcus aureus
Genetic Insert: Glutathione-S-Transferase/LaG16/MNase
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4304; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34644460
Proper citation: RRID:Addgene_170978 Copy
Vector Backbone Description: Backbone Size:4103; Vector Backbone:pCS2+ (modified); Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Comments: pCS2+ has been modified by the deletion of the AC preceeding the T7 promoter addition of the sequence TTCTGCAGCCC immediately following the XbaI site. The PstI site added to CS2+ is encompassed in the sequence (CTGCAG).
Modifications were made by Robert Davis of Marc Kirshner's lab to improve the efficiency of the transcription from the T7 promoter.
Proper citation: RRID:Addgene_17095 Copy
Vector Backbone Description: Backbone Size:5090; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 2005. Fire Lab Miniprep Number pPD158.87, Ligation number L5767.
Proper citation: RRID:Addgene_1709 Copy
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