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Species: Homo sapiens
Genetic Insert: OPN-NT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pDest490; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17452979
Proper citation: RRID:Addgene_17594 Copy
Species: Homo sapiens
Genetic Insert: ZBED9-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175854 Copy
Species: Homo sapiens
Genetic Insert: CHCHD3-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175857 Copy
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:SITS-mCherry-ccdb (Addgene 118629); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176024 Copy
Genetic Insert: rtTA3
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32944247
Proper citation: RRID:Addgene_176027 Copy
Species: Homo sapiens
Genetic Insert: CDX4-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175852 Copy
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:SITS-EGFP-ccdb (Addgene 118628); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176020 Copy
Species: Homo sapiens
Genetic Insert: EF-1a promoter
Vector Backbone Description: Backbone Marker:Dr. Didier Trono; Backbone Size:0; Vector Backbone:SIN LV pRLLhPGK.GFP Sin-18 (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11781219
Comments: SIN lentiviral vector that expresses GFP controlled by the promoter of the housekeeping gene EF-1alpha.
We constructed EF.GFP by replacing the human PGK promoter (EcoRV to BamHI sites in the PGK.GFP vector) with human EF-1alpha promoter from plasmid pEF1/Myc-His-A (Invitrogen, Carlsbad, CA). The EF-1alpha promoter (nucleotide 373 to 1560 as numbered in GenBank accession J04616) contains the first exon, first intron, and part of the second exon before the coding sequence (starting at nucleotide 1582).
Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.
Proper citation: RRID:Addgene_17616 Copy
Vector Backbone Description: Vector Backbone:MoClo assembly; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015) from the part plasmids pYTK008, pYTK047, pYTK073, pYTK090, pMC6-spacer, pMC7-XII5dw, pMC8b-XII5up. Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176161 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g9608.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176078 Copy
Species: Drosophila melanogaster
Genetic Insert: L596_g25050.t1
Vector Backbone Description: Backbone Size:8220; Vector Backbone:Addgene #104968; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34714893
Proper citation: RRID:Addgene_176079 Copy
Species: Homo sapiens
Genetic Insert: PBZ-C
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34731617
Proper citation: RRID:Addgene_176070 Copy
Species: Homo sapiens
Genetic Insert: WWE
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34731617
Proper citation: RRID:Addgene_176072 Copy
Species: Homo sapiens
Genetic Insert: LivePAR (Y107A)
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34731617
Proper citation: RRID:Addgene_176073 Copy
Species: Homo sapiens
Genetic Insert: Light chain of Fab_8D3_2
Vector Backbone Description: Backbone Marker:addgene; Backbone Size:4879; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34620716
Proper citation: RRID:Addgene_176076 Copy
Species: Homo sapiens
Genetic Insert: His6 tagged heavy chain of Fab_8D3_2
Vector Backbone Description: Backbone Marker:addgene; Backbone Size:4879; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34620716
Proper citation: RRID:Addgene_176075 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728840
Comments: The wild-type ubiquitin sequence in this plasmid is: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG
Proper citation: RRID:Addgene_17608 Copy
Species: Homo sapiens
Genetic Insert: Paxillin
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34084168
Proper citation: RRID:Addgene_176107 Copy
Species: Homo sapiens
Genetic Insert: PBM
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34731617
Proper citation: RRID:Addgene_176069 Copy
Species: Homo sapiens
Genetic Insert: ITSN1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37449837
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176103 Copy
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