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Species: Staphylococcus aureus
Genetic Insert: SaCas9
Vector Backbone Description: Backbone Marker:Feng Zhang's Laboratory; Backbone Size:7447; Vector Backbone:pX601; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27601644
Proper citation: RRID:Addgene_84040 Copy
Vector Backbone Description: Vector Backbone:pCRISPRia-v2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661255
Proper citation: RRID:Addgene_83992 Copy
Species: Homo sapiens
Genetic Insert: YAP1 (siRNA insensitive)
Vector Backbone Description: Backbone Marker:Piccolo lab; Backbone Size:8500; Vector Backbone:FUW-tetO-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27641305
Comments: The insert (FLAG-hYAP1 S94A siRNA insensitive) was generated by PCR from FLAG-hYAP1wt siRNA insensitive cDNA (Addgene #84009) by point mutagenesis.
Proper citation: RRID:Addgene_84010 Copy
Vector Backbone Description: Backbone Size:8482; Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27641305
Proper citation: RRID:Addgene_84008 Copy
Vector Backbone Description: Vector Backbone:pCRISPRia-v2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661255
Proper citation: RRID:Addgene_84002 Copy
Species: Staphylococcus aureus
Genetic Insert: SaCas9
Vector Backbone Description: Backbone Marker:Feng Zhang's laboratory; Backbone Size:7447; Vector Backbone:pX601; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27601644
Proper citation: RRID:Addgene_84039 Copy
Vector Backbone Description: Backbone Size:10000; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25122746
Proper citation: RRID:Addgene_84034 Copy
Vector Backbone Description: Backbone Marker:Wellcome trust Sanger Institute; Vector Backbone:5'-PTK-3'; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24927590
Proper citation: RRID:Addgene_84036 Copy
Vector Backbone Description: Vector Backbone:pCRISPRia-v2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661255
Proper citation: RRID:Addgene_83971 Copy
Vector Backbone Description: Vector Backbone:pCRISPRia-v2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661255
Proper citation: RRID:Addgene_83973 Copy
Vector Backbone Description: Vector Backbone:pCRISPRia-v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661255
Proper citation: RRID:Addgene_83978 Copy
Species: Homo sapiens
Genetic Insert: SRSF2
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Backbone Size:6651; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25965569
Proper citation: RRID:Addgene_84020 Copy
Species: Homo sapiens
Genetic Insert: SRSF2
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Backbone Size:6651; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25965569
Proper citation: RRID:Addgene_84023 Copy
Vector Backbone Description: Vector Backbone:pSicoR; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23739767
Proper citation: RRID:Addgene_84219 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:5081; Vector Backbone:GeneArt CRISPR Nuclease vector with OFP reporter; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27618190
Comments: The four copies of ERT2 are identical in sequence and may recombine occasionally. Hence, it best to use competent cells like STBL3 or NEB Stable to propagate the plasmid and to also check the plasmid size on an agarose gel.
Proper citation: RRID:Addgene_84232 Copy
Vector Backbone Description: Vector Backbone:pSicoR; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26080438
Proper citation: RRID:Addgene_84220 Copy
Species: Synthetic
Genetic Insert: Sa sgTRE3G
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27776111
Comments: Contains a CMV-mCherry fluorescent marker 3' of the sgRNA.
Proper citation: RRID:Addgene_84250 Copy
Species: Mus musculus
Genetic Insert: Isl1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27243442
Comments: SphI or SacII digestion and SP6 RNA Polymerase for antisense probe
Proper citation: RRID:Addgene_84311 Copy
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:10473; Vector Backbone:pYES1L; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27540174
Proper citation: RRID:Addgene_84301 Copy
Species: Synthetic
Genetic Insert: P2A-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27358232
Proper citation: RRID:Addgene_84329 Copy
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