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Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has aminoglycosyl phosphotransferase driven by 35S promoter (G418 resistance). We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113743 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has hygromycinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113742 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has zeocinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113744 Copy
Species: venezuelan equine encephalitis
Genetic Insert: Lvl2 (nsP2 Q739L)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115960 Copy
Species: Synthetic
Genetic Insert: Lvl0 SGP (+/- Apt) Dest
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115950 Copy
Species: Synthetic
Genetic Insert: Lvl0 ORF Dest
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115951 Copy
Vector Backbone Description: Vector Backbone:pMH-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28325877
Proper citation: RRID:Addgene_101765 Copy
Vector Backbone Description: Vector Backbone:pMH-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28325877
Proper citation: RRID:Addgene_101768 Copy
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30639212
Comments: Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to depositor's provided sequence for this section of the plasmid.
Proper citation: RRID:Addgene_120352 Copy
Species: E.coli
Genetic Insert: ccdB Cassette (BsaI)
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, Binary, Module 1; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31085714
Proper citation: RRID:Addgene_126077 Copy
Species: E.coli
Genetic Insert: ccdB Cassette (BsaI)
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression, Binary, Module 1; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31085714
Proper citation: RRID:Addgene_126210 Copy
Species: E.coli
Genetic Insert: ccdB Cassette (BsaI)
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression, Binary, Module 1; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31085714
Proper citation: RRID:Addgene_126209 Copy
Species: Synthetic
Genetic Insert: mRFP-T2A-BlasticidinR
Vector Backbone Description: Vector Backbone:modified PB-CA (Addgene 20960) ; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_137881 Copy
Genetic Insert: Cherry
Vector Backbone Description: Backbone Size:5778; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: There is a H30R mutation in mCherry. This mutation does not affect plasmid function and the construct has been validated for expression.
Proper citation: RRID:Addgene_13075 Copy
Vector Backbone Description: Backbone Marker:Susan Lindquist; Backbone Size:8781; Vector Backbone:pAG426-GAL-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: pAG426-GAL-ccdB-mKate2, plasmid for overexpression of prion domains to induce prions. Perform an LR reaction to yield an expression clone.
Proper citation: RRID:Addgene_131123 Copy
Vector Backbone Description: Backbone Marker:Dirk Landgraf; Backbone Size:5056; Vector Backbone:pDML112; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty basic yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pGAN147. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.
Proper citation: RRID:Addgene_129202 Copy
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134979 Copy
Vector Backbone Description: Backbone Size:5288; Vector Backbone:pOO2-GW; Vector Types:bacterial expression vector for generating SP6 transcripts in vitro for Xenopus oocyte transfection; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21107422
Comments: The AtAMT1;1 coding sequence with the att sites (attB1 and attB2) from the pDRf1-AtAMT1;1 (Loque et al 2007 Nature 446 195-198) was remobilized with both restriction enzymes SpeI and XhoI then subsequently cloned between SpeI and XhoI sites in the p6013-002 (Ludewig et al., 2002 J. Biol. Chem. 277, 13548-13555). The AtAMT1;1 coding sequence was then replaced by the Gateway DNA cassette after a BP reaction with pDONR 221 (GATEWAY technology, Invitrogen) to generate the p002-GW vector.
Proper citation: RRID:Addgene_135097 Copy
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pMH-EF1-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32611815
Proper citation: RRID:Addgene_133018 Copy
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pMH-CMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32611815
Proper citation: RRID:Addgene_133017 Copy
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