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Species: Synthetic
Genetic Insert: Puromycin
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62164 Copy
Species: Synthetic
Genetic Insert: scramble sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62285 Copy
Species: Synthetic
Genetic Insert: epNTR
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24848468
Proper citation: RRID:Addgene_62213 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62170 Copy
Species: Synthetic
Genetic Insert: CreERT2
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62174 Copy
Species: Synthetic
Genetic Insert: sgRNA
Vector Backbone Description: Backbone Size:2000; Vector Backbone:Trc99a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25636838
Proper citation: RRID:Addgene_62226 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62184 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62185 Copy
Species: Synthetic
Genetic Insert: ER-mRFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16617114
Comments: Red localization marker for mammalian endoplasmic reticulum.
Proper citation: RRID:Addgene_62236 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Size:5385; Vector Backbone:p414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36325175
Comments: This plasmid is compatible with gRNA expression plasmids for budding yeast, such as Addgene Plasmid #43803 ( http://www.addgene.org/43803 ).
Proper citation: RRID:Addgene_62190 Copy
Species: Synthetic
Genetic Insert: hamster BiP-mGFP-KDEL
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20410136
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-GFP if you simply want a marker for the ER when imaging cells.
Proper citation: RRID:Addgene_62231 Copy
Species: Synthetic
Genetic Insert: hamster BiP-mGFP-KDEL
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20410136
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
This mutant can not bind client proteins.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-GFP if you simply want a marker for the ER when imaging cells.
Proper citation: RRID:Addgene_62232 Copy
Species: Synthetic
Genetic Insert: hamster BiP-mCherry-KDEL
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20410136
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-mCherry if you simply want a marker for the ER when imaging cells.
Proper citation: RRID:Addgene_62233 Copy
Species: Synthetic
Genetic Insert: VP64dCas9BFPVP64
Vector Backbone Description: Vector Backbone:TetO-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25448066
Comments: The vector backbone was from Addgene plasmid 27152 (PI Marius Wernig)
The dCas9:BFP fusion part of the insert was cloned from Addgene plasmid 44247 (PI Stanley Qi)
Proper citation: RRID:Addgene_62195 Copy
Species: Synthetic
Genetic Insert: Left CLYBL TALEN
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pZT; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25587899
Comments: pZT-C13/CLYBL TALENs were assembled using RVD monomers from Golden Gate TALEN kit 1.0 (TALEN Kit #1000000016) and a mammalian TALEN expression vector pZT
Proper citation: RRID:Addgene_62196 Copy
Species: Synthetic
Genetic Insert: A2T sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62258 Copy
Species: Synthetic
Genetic Insert: A1NT
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62255 Copy
Species: Synthetic
Genetic Insert: A1T sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62256 Copy
Species: Synthetic
Genetic Insert: su9-tagBFP
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62383 Copy
Species: Synthetic
Genetic Insert: tev 3x FLAG
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCRII blunt topo; Vector Types:yeast c-terminal tagging vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62384 Copy
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