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Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Prof. Amir Sharon (Tel Aviv University, Israel); Vector Backbone:pSH1; Vector Types:Filamentous Ascomycetes EGFP expression; Bacterial Resistance:Ampicillin
Comments: Plasmid provided by Prof. Amir Sharon (Tel Aviv University, Israel)
The full plasmid sequence is assembled and not the result of sequencing, there are some discrepancies between the Addgene QC sequence and the full plasmid sequence. These differences are in the plasmid backbone regions.
Proper citation: RRID:Addgene_42323 Copy
Species: Homo sapiens
Genetic Insert: VAMP 3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9614185
Comments: Full-length cDNA of the human isoform of cellubrevin, was obtained by reverse transcription-PCR on CaCo-2 mRNA using standard procedures and the following set of oligonucleotides:
5'-ATGTCTACAGGTCCAACTGCTG-3' (hcb5')
and
5'-GCTGGTTCTTCATGAAGAGACA-3' (hcb3')
Proper citation: RRID:Addgene_42310 Copy
Species: Homo sapiens
Genetic Insert: PARP15A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/3BLJ/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42398 Copy
Species: Homo sapiens
Genetic Insert: VAMP 4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11839770
Proper citation: RRID:Addgene_42313 Copy
Species: Homo sapiens
Genetic Insert: STARD5A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2R55/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_42392 Copy
Genetic Insert: VAMP 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12853575
Comments: The V8A & T116S substitutions found in the Addgene sequence correspond to polymorphisms and have no functional impact.
Proper citation: RRID:Addgene_42308 Copy
Species: E. coli O157:H7
Genetic Insert: ChuA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3418; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21171606
Proper citation: RRID:Addgene_42539 Copy
Species: Arabidopsis thaliana
Genetic Insert: FKF1 (N-LOV, G128D mutant) fused with VP16
Vector Backbone Description: Backbone Marker:Invitrogene; Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801976
Proper citation: RRID:Addgene_42499 Copy
Species: Homo sapiens
Genetic Insert: shRNA-PKM2
Vector Backbone Description: Backbone Marker:The RNAi consortium; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: The depositing scientist notes: NM_011099.3 is PKM2 and NM_001253883.1 is PKM1, based on sequence homology of the respective translated products to the human PKM isoforms. The isoform annotations in the corresponding GeneBank entries are incorrect.
Proper citation: RRID:Addgene_42516 Copy
Species: Arabidopsis thaliana
Genetic Insert: GIGANTEA fused with Gal4 DBD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801976
Proper citation: RRID:Addgene_42500 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23245941
Proper citation: RRID:Addgene_42555 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Marker:TRC; Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23245941
Comments: TRCN0000107265
Proper citation: RRID:Addgene_42541 Copy
Species: Homo sapiens
Genetic Insert: CTNNB1
Vector Backbone Description: Backbone Marker:TRC; Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23245941
Comments: TRCN0000003845
Proper citation: RRID:Addgene_42544 Copy
Species: Homo sapiens
Genetic Insert: TLR4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19085953
Proper citation: RRID:Addgene_42646 Copy
Species: Homo sapiens
Genetic Insert: TLR4 299 SNP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19085953
Proper citation: RRID:Addgene_42647 Copy
Species: Homo sapiens
Genetic Insert: Human H4
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:Pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42633 Copy
Species: Homo sapiens
Genetic Insert: EEA1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22308388
Proper citation: RRID:Addgene_42635 Copy
Species: Homo sapiens
Genetic Insert: TCF7L2
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLX303; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23245941
Proper citation: RRID:Addgene_42592 Copy
Species: Homo sapiens
Genetic Insert: hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase
Vector Backbone Description: Backbone Marker:Stanley Lemon (PubMed ID 10611164); Vector Backbone:pRL-HL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15123638
Comments: phpRL-BCL2-FL(pA) includes a 143-bp stable RNA hairpin located upstream of renilla luciferase, BCL2 5'UTR and firefly luciferase. See plasmid map link above for more details.
This plasmid is one of several reporter constructs created to assess potential IRES activity of the BCL2 5'UTR. The hairpin in this construct inhibits translation of renilla luciferase and to a lesser extent translation of firefly luciferase.
Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.
Proper citation: RRID:Addgene_42595 Copy
Species: Hepatitis B virus
Genetic Insert: Hepatitis B virus protein X
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23091012
Proper citation: RRID:Addgene_42596 Copy
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