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Species: Other
Genetic Insert: Integrase 5 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 5 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127531 Copy
Species: Other
Genetic Insert: Integrase 4 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 4 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127530 Copy
Species: Other
Genetic Insert: Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pBluescript II SK(-); Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original Bxb1 coding sequence (before A. thaliana codon optimization) was obtained from Bonnet, J. et al. Science 340, 599-603 (2013) (Genbank KC529324.1).
Proper citation: RRID:Addgene_127536 Copy
Species: Other
Genetic Insert: Integrase 5 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pUB-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The integrase 5 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127514 Copy
Species: Other
Genetic Insert: Integrase 4 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pUB-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The integrase 4 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127513 Copy
Species: Other
Genetic Insert: Integrase phiC31 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pBluescript II SK(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The UbC promoter and _-globin poly(A) signal sequences are derived from pUB-GFP plasmid (Addgene #11155).
Proper citation: RRID:Addgene_127518 Copy
Species: Other
Genetic Insert: Integrase 13 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pUB-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The integrase 13 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127517 Copy
Species: Other
Genetic Insert: Sindbis Glycoproteins
Vector Backbone Description: Vector Backbone:pSin; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31280962
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_127694 Copy
Species: Other
Genetic Insert: Sindbis Capsid
Vector Backbone Description: Vector Backbone:pSin; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31280962
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_127693 Copy
Species: Other
Genetic Insert: Sindbis Structural Genome
Vector Backbone Description: Vector Backbone:pSRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31280962
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_127692 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pLenti-C-MYC-DDK Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30373810
Proper citation: RRID:Addgene_123299 Copy
Species: Other
Genetic Insert: ChromeQ-GFP
Vector Backbone Description: Backbone Marker:original from Stratagene; Backbone Size:4708; Vector Backbone:pAAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30610117
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_123317 Copy
Species: Other
Genetic Insert: Staphylococcus aureus Cas9
Vector Backbone Description: Vector Backbone:pSP72; Vector Types:CRISPR, Leishmania; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31434745
Proper citation: RRID:Addgene_123262 Copy
Species: Other
Genetic Insert: PpsbB
Vector Backbone Description: Vector Backbone:pMK-RQ; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. The insert is a chloroplast promoter designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123481 Copy
Species: Other
Genetic Insert: PpsbD-leader
Vector Backbone Description: Vector Backbone:pMK-T; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. The insert is a chloroplast promoter designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123482 Copy
Species: Other
Genetic Insert: PrbcL
Vector Backbone Description: Vector Backbone:pMK-T; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. The insert is a chloroplast promoter designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123485 Copy
Species: Other
Genetic Insert: PrbcL (-110 to +9)
Vector Backbone Description: Vector Backbone:pMK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. The insert is a chloroplast promoter designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123487 Copy
Species: Other
Genetic Insert: PrbcL (core -35 to +9)
Vector Backbone Description: Vector Backbone:pMK-RQ; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. The insert is a chloroplast promoter designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123486 Copy
Species: Other
Genetic Insert: Prrn16
Vector Backbone Description: Vector Backbone:pMK-RQ; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. The insert is a chloroplast promoter designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123489 Copy
Species: Other
Genetic Insert: mPrrn::RBS (60%)
Vector Backbone Description: Vector Backbone:pMK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. The insert is a chloroplast promoter::5'UTR fusion designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123517 Copy
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