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Species: Homo sapiens
Genetic Insert: Huntingtin Exon 1
Vector Backbone Description: Backbone Marker:eBioLabs; Backbone Size:5800; Vector Backbone:pTWIN1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27002149
Proper citation: RRID:Addgene_84347 Copy
Species: Streptococcus pyogenes
Genetic Insert: dcas9
Vector Backbone Description: Backbone Marker:Addgene 20315; Backbone Size:4640; Vector Backbone:pTC-0X-1L; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27407107
Proper citation: RRID:Addgene_84379 Copy
Vector Backbone Description: Backbone Size:9093; Vector Backbone:pLenti PGK PURO DEST (Addgene 19068); Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_84372 Copy
Species: Mus musculus
Genetic Insert: SIRT1
Vector Backbone Description: Backbone Size:9220; Vector Backbone:pAd-Track-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15744310
Proper citation: RRID:Addgene_8439 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:3834; Vector Backbone:pMuLE Dest BlastR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30263951
Proper citation: RRID:Addgene_84384 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:3834; Vector Backbone:pMuLE Dest BlastR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30061623
Proper citation: RRID:Addgene_84383 Copy
Species: Homo sapiens
Genetic Insert: guide RNA
Vector Backbone Description: Backbone Marker:Addgene 44248; Vector Backbone:gRNA-humanized; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27662091
Proper citation: RRID:Addgene_84477 Copy
Genetic Insert: ArchT
Vector Backbone Description: Backbone Marker:Rachael Neve; Vector Backbone:Derived from AAV V032; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661450
Comments: For more info regarding the plasmid, see RAM manuscript on eLife's homepage: http://dx.doi.org/10.7554/eLife.13918
Proper citation: RRID:Addgene_84472 Copy
Genetic Insert: Channelrhodopsin 2
Vector Backbone Description: Backbone Marker:Rachael Neve; Vector Backbone:Derived from AAV V032; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661450
Comments: For more info regarding the plasmid, see RAM manuscript on eLife's homepage: http://dx.doi.org/10.7554/eLife.13918
Proper citation: RRID:Addgene_84471 Copy
Genetic Insert: mKate2
Vector Backbone Description: Backbone Marker:Rachael Neve; Vector Backbone:Derived from AAV V032; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661450
Comments: For more info regarding the plasmid, see RAM manuscript on eLife's homepage: http://dx.doi.org/10.7554/eLife.13918
Proper citation: RRID:Addgene_84474 Copy
Species: Homo sapiens
Genetic Insert: dCas9-Tet1CD
Vector Backbone Description: Backbone Size:9213; Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27662091
Proper citation: RRID:Addgene_84475 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Rachael Neve; Vector Backbone:Derived from AAV V032; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661450
Comments: The RAM-EGFP vector is not published, but confirmed experimentally. For more info regarding other RAM plasmid, see RAM manuscript on eLife's homepage: http://dx.doi.org/10.7554/eLife.13918
Proper citation: RRID:Addgene_84469 Copy
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Rachael Neve; Vector Backbone:Derived from AAV V032; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27661450
Comments: Please note that mutation T230I in TdTomato was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
For more info regarding the plasmid, see RAM manuscript on eLife's homepage: http://dx.doi.org/10.7554/eLife.13918
Proper citation: RRID:Addgene_84468 Copy
Species: Synthetic
Genetic Insert: FLIP - Puro
Vector Backbone Description: Backbone Marker:TAKARA; Vector Backbone:pUC118; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28135257
Proper citation: RRID:Addgene_84538 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pUMVC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12649500
Comments: Packaging plasmid for MuLV constructs, MuLV gag and pol (no envelope). The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html
See pUMVC3-gag-pol for a map/sequence.
Proper citation: RRID:Addgene_8449 Copy
Species: Mus musculus
Genetic Insert: Mouse c-myc (exons II and III)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8455630
Proper citation: RRID:Addgene_8448 Copy
Species: Homo sapiens
Genetic Insert: CASTOR1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26972053
Proper citation: RRID:Addgene_84488 Copy
Species: Stigeoclonium helveticum
Genetic Insert: Chronos-tdTomato
Vector Backbone Description: Backbone Marker:original from Stratagene; Backbone Size:4684; Vector Backbone:AAV-Syn-FLEX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_84481 Copy
Species: Chlamydomonas noctigama
Genetic Insert: ChrimsonR-GFP
Vector Backbone Description: Backbone Marker:original from Stratagene; Backbone Size:4684; Vector Backbone:AAV-Syn-FLEX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_84480 Copy
Species: Stigeoclonium helveticum
Genetic Insert: Chronos-GFP
Vector Backbone Description: Backbone Marker:original from Stratagene; Backbone Size:4670; Vector Backbone:AAV-Syn-FLEX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_84482 Copy
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