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Plasmids are provided by Addgene and DGRC.

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pGGB006
 
Resource Report
Resource Website 		RRID:Addgene_48823 signal sequence ER Synthetic Ampicillin PMID:24376629 This plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp ER signal sequence = 2252-2320bp BsaI site #2 = 2321-2331bp Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:15:54 0
pGGB005
 
Resource Report
Resource Website 		RRID:Addgene_48822 SV40 nuclear localization signal Synthetic Ampicillin PMID:24376629 This plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp SV40 nuclear localization signal = 2252-2275bp BsaI site #2 = 2276-2286bp Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:15:53 0
pGGB001
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_48819 mCherry:Linker Synthetic Ampicillin PMID:24376629 This plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp mCherry-linker = 2252-3060bp BsaI site #2 = 3061-3071bp Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:15:53 1
pZSm45 sodA
 
Resource Report
Resource Website 		RRID:Addgene_48890 sodA LuxR Spectinomycin PMID:22178928 Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:15:54 0
pTD103luxI_sfGFP -R
 
Resource Report
Resource Website 		RRID:Addgene_48887 sfGFP and luxI Kanamycin PMID:22178928 * The presence of glucose will reduce the amount of expression from the Lux promoter driving GFP and other genes. Omitting the glucose could potentially make the cell overburdened. Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:15:54 0
EcNGRfEfC-R6M1G7
 
Resource Report
Resource Website 		RRID:Addgene_48924 galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG Spectinomycin strain contains pZE41 backbone (specR), fadD, tesA' galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin 2026-08-15 01:15:55 0
EcNGRfEfC-R6M1E6
 
Resource Report
Resource Website 		RRID:Addgene_48923 galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG Spectinomycin strain contains pZE41 backbone (specR), fadD, tesA' galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin 2026-08-15 01:15:54 0
pZSm45 ndhII
 
Resource Report
Resource Website 		RRID:Addgene_48889 ndhII and LuxR Spectinomycin PMID:22178928 Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:15:54 0
EcNGRfEfC-R6M1E2
 
Resource Report
Resource Website 		RRID:Addgene_48922 galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG Spectinomycin strain contains pZE41 backbone (specR), fadD, tesA' galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin 2026-08-15 01:15:54 0
pTD103aiiA(Cm) -R
 
Resource Report
Resource Website 		RRID:Addgene_48888 aiiA Chloramphenicol PMID:22178928 Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:15:55 0
EcNGRfEfC-R6M1D4
 
Resource Report
Resource Website 		RRID:Addgene_48921 galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG Spectinomycin strain contains pZE41 backbone (specR), fadD, tesA' galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin 2026-08-15 01:15:55 0
pJS169
 
Resource Report
Resource Website 		RRID:Addgene_48882 lacI with ssrA tag Ampicillin PMID:18971928 Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:15:54 0
pFBXC3H
 
Resource Report
Resource Website 		RRID:Addgene_49050 Chloramphenicol and Ampicillin PMID:26043233 Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland. Backbone Marker:Imre Berger; Backbone Size:6992; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:15:55 0
pDONR221-Phelo
 
Resource Report
Resource Website 		RRID:Addgene_48995 Sh ble Kanamycin PMID:19432966 Please note that Addgene's sequencing results found a few differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing laboratory, these differences are not a concern for the function of the plasmid. Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2500; Vector Backbone:pDONR221; Vector Types:Gateway attB1 & attB2; Bacterial Resistance:Kanamycin 2026-08-15 01:15:55 0
pFBXNH3
 
Resource Report
Resource Website 		RRID:Addgene_49049 Chloramphenicol and Ampicillin PMID:26043233 Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland. Backbone Marker:Imre Berger; Backbone Size:6986; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:15:56 0
C321.ΔA
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_48998 Recoded E. coli genome with all instances of the UAG codon and RF1 removed (UAG termination function removed) E. coli Ampicillin PMID:24136966 E. coli MG1655 Delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta prfA Delta mutS::zeoR; all 321 UAG codons changed to UAA This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin. A mutS+ version of this strain will be deposited with addgene soon. Genome sequence deposited at NCBI (accession # CP006698) Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin See genbank file 2026-08-15 01:15:56 13
EMM71T
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_49044 Ampicillin PMID:24013198 Backbone Marker:Joung Lab; Backbone Size:8550; Vector Backbone:unknown; Vector Types:Mammalian Expression, TALE LSD1 Expression Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:15:55 1
EMM65
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_49042 Ampicillin PMID:24013198 Backbone Marker:Joung Lab; Backbone Size:8550; Vector Backbone:unknown; Vector Types:Mammalian Expression, TALE LSD1 Expression Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:15:56 4
pDONR221-Neo
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_48993 aminoglycoside 3' phosphotransferase Kanamycin PMID:19432966 Please note that Addgene's sequencing results found a few differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing laboratory, these differences are not a concern for the function of the plasmid. Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2500; Vector Backbone:pDONR221; Vector Types:Gateway attB1 & attB2; Bacterial Resistance:Kanamycin 2026-08-15 01:15:56 1
pET-15b-EK_C122S_His5
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_49048 Enteropeptidase, peptidase domain Bos taurus Ampicillin PMID:24184090 Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin P92R, E185D, C122S 2026-08-15 01:15:55 4

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