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Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12649500
Comments: Empty lentiviral vector for siRNA expression; replaces Lentihair; see author's map. The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html
Note that Addgene's quality control sequence shows that there are about 25 nucleotides between the AgeI and EcoRI sites (the depositor sequences is not as accurate in this region).
For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454). For the official vector of The RNAi Consortium and a plasmid map, please see plasmid #10878.
Proper citation: RRID:Addgene_8453 Copy
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2623; Vector Backbone:pUC19; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27989123
Proper citation: RRID:Addgene_84610 Copy
Vector Backbone Description: Vector Backbone:pUS116; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: pUS116 is an E.coli/Mycobacterium shuttle vector, with high copy number in E.coli and low copy number in Mycobacterium. The plasmid has unique NdeI, BamHI, PvuII, EcoRI, NheI sites for cloning, and genes cloned at this MCS can be expressed via acetamide induction in Mycobacterium, but this system doesnt work in E.coli. This plasmid was made by deletion of the recombineering genes from pJV53 (see van Kessel and Hatfull, 2007), to re-forma basic shuttle/expression vector. pUS116 is equivalent to vector pLAM12.
Proper citation: RRID:Addgene_84578 Copy
Genetic Insert: hrGFP
Vector Backbone Description: Backbone Size:2623; Vector Backbone:pUC19; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27989123
Proper citation: RRID:Addgene_84616 Copy
Genetic Insert: hrGFP
Vector Backbone Description: Backbone Size:2623; Vector Backbone:pUC19; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27989123
Proper citation: RRID:Addgene_84617 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6100; Vector Backbone:pMRX-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27818143
Proper citation: RRID:Addgene_84572 Copy
Vector Backbone Description: Backbone Size:7547; Vector Backbone:pMuLE Lenti Dest Neo; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30061623
Proper citation: RRID:Addgene_84574 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:K. Kawakami lab; Vector Backbone:pT2KXIG; Vector Types:Zebrafish transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25068273
Comments: The amino acid sequence of the Odc1-derived destabilizing element is: SHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV.
Proper citation: RRID:Addgene_84603 Copy
Species: Homo sapiens
Genetic Insert: Rac1
Vector Backbone Description: Backbone Marker:Iain Fraser (Addgene plasmid # 25734); Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25044255
Proper citation: RRID:Addgene_84605 Copy
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2623; Vector Backbone:pUC19; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27989123
Proper citation: RRID:Addgene_84608 Copy
Species: Mus musculus
Genetic Insert: FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
Vector Backbone Description: Backbone Size:6255; Vector Backbone:pUbC lentivirus expression vector (2nd generation); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27313041
Proper citation: RRID:Addgene_84561 Copy
Species: Synthetic
Genetic Insert: Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
Vector Backbone Description: Vector Backbone:pUbC lentivirus expression vector (2nd generation); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27313041
Proper citation: RRID:Addgene_84563 Copy
Species: Homo sapiens
Genetic Insert: MLCK
Vector Backbone Description: Backbone Marker:Iain Fraser (Addgene plasmid # 25734); Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25634210
Comments: The MLCK insert starts at amino acid 885 and contains D899E, D914A, N1782T, K1785R, and S1788T amino acid mutations compared to best match reference sequence (NP_444253.3).
Proper citation: RRID:Addgene_84647 Copy
Genetic Insert: mCyRFP1
Vector Backbone Description: Backbone Size:2834; Vector Backbone:pNCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798609
Proper citation: RRID:Addgene_84545 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12649500
Comments: Packaging plasmid for Lentiviral constructs (vpr is removed). The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html
*Addgene Comment: This plasmid contains some HIV accessory genes. Please reach out to your biosafety officer for guidance. The definition of "2nd generation" for this plasmid differs from the standard definition.
Proper citation: RRID:Addgene_8455 Copy
Species: Acidaminococcus_sp_BV3L6
Genetic Insert: AsCpf1
Vector Backbone Description: Backbone Size:8700; Vector Backbone:lentiCas9-Blast(Addgene#:52962); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27992409
Proper citation: RRID:Addgene_84750 Copy
Species: Synthetic
Genetic Insert: huLbCpf1
Vector Backbone Description: Backbone Size:8069; Vector Backbone:pHKO_23; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27918548
Comments: Contains a BsmBI cloning cassette for insertion of Cpf1 guides under U6 promoter control.
Proper citation: RRID:Addgene_84740 Copy
Species: Homo sapiens
Genetic Insert: Ngn2 and Mash1 with a T2A
Vector Backbone Description: Backbone Marker:clonetech; Backbone Size:7791; Vector Backbone:PLVX TIGHT PURO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26456686
Proper citation: RRID:Addgene_84777 Copy
Vector Backbone Description: Backbone Marker:Luigi Naldini , PMID 15619618; Vector Backbone:BdLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633241
Proper citation: RRID:Addgene_84771 Copy
Species: Other
Genetic Insert: LFn
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27045008
Proper citation: RRID:Addgene_84872 Copy
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