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Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Ser650Asp mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: Mutation in Flag-tagged AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171246 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Ser650Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: Mutation in Flag-tagged AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171245 Copy
Species: Synthetic
Genetic Insert: J306 scRNA
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:6261; Vector Backbone:pPPC020.306; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK134, pBBR1-GmR_J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
Proper citation: RRID:Addgene_171149 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 beta catalytic subunit
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The construction is based on PCR amplification using Pfu DNA polymerase.
Proper citation: RRID:Addgene_171269 Copy
Species: Synthetic
Genetic Insert: hAAVS1 scRNA
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:4577; Vector Backbone:pRK2-KmR; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK080, pRK2-KmR_J1-BBa_J23117-mRFP_hAAVS1.
Proper citation: RRID:Addgene_171147 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: The construction is based on PCR amplification using Pfu DNA polymerase and DNA mutagenesis.
Proper citation: RRID:Addgene_171268 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 alpha catalytic subunit
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: The construction is based on PCR amplification using Pfu DNA polymerase.
Proper citation: RRID:Addgene_171267 Copy
Species: Synthetic
Genetic Insert: J109 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:5148; Vector Backbone:pBBR1-MCS2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK058, pBBR1-KmR_J1-mRFP_J109
Proper citation: RRID:Addgene_171145 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Lys639Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Flag-tagged human AR Lysine mutant, and the exact amino position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171264 Copy
Species: Synthetic
Genetic Insert: J106 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:5148; Vector Backbone:pBBR1-MCS2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK033.06, pBBR1-KmR_J106
Proper citation: RRID:Addgene_171142 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Lys630Ala-Lys632Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Flag-tagged human AR Lysine mutant, and the exact amino position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171263 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Arg407Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Flag-tagged human AR Arginine mutant, and the exact amino position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171262 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Lys313Ala-Lys318Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Flag-tagged human AR Lysine mutant, and the exact amino position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171260 Copy
Species: Synthetic
Genetic Insert: J1-BBa_J23117-sfGFP
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Backbone Size:4569; Vector Backbone:pUC18TminiTn7T-Gm; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCDP009, pUC18TminiTn7T-Gm_J1-BBa_J23117-sfGFP_Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A/S101A)
Used for conjugation, with pTNS1 and pRK2013 helper plasmids, to integrate J1-BBa_J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) cassettes into gram-negative bacteria.
Point mutation in the FRT site was observed and remained applicable with counterselection by pCK255
Proper citation: RRID:Addgene_171139 Copy
Species: Homo sapiens
Genetic Insert: RBM8A 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGlo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34944051
Proper citation: RRID:Addgene_171136 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Lys222Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Flag-tagged human AR Lysine mutant, and the exact amino position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171257 Copy
Species: Homo sapiens
Genetic Insert: RBM8A 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGlo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34944051
Proper citation: RRID:Addgene_171135 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-tagged human androgen receptor Arg129Ala mutant
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Flag-tagged human AR Arginine mutant, and the exact amino position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
Proper citation: RRID:Addgene_171256 Copy
Species: Xenopus laevis
Genetic Insert: beta-globin 5' UTR
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCD-PS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Description: This is an old but serviceable SV40 expression construct (pCD-PS) modified to give the Xenopus beta-globin untranslated regions and multiple cloning sites from pT7TS vector (Addgene plasmid 17091).
Reference: The vector was made by Julia Yang-Snyder.
Proper citation: RRID:Addgene_17120 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:NA; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Description: Expression vector that is identical to CS2+ and CS3+ except that the CMV promoter has been removed, and replaced with an SV40 promoter fragment (SalI-SmaI) from pCD-PS.
Reference: The vector was made by Julia Yang-Snyder.
Proper citation: RRID:Addgene_17122 Copy
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