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Species: Mus musculus
Genetic Insert: L7-6, Purkinje cell specific promoter
Vector Backbone Description: Backbone Size:7914; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28828391
Comments: Please note that the Addgene verified sequence contains a truncated ITR compared to the depositor's reference sequence. This truncation was present in the original stock and still worked well for AAV packaging.
Proper citation: RRID:Addgene_126462 Copy
Species: Mus musculus
Genetic Insert: Fndc3b
Vector Backbone Description: Vector Backbone:pcDNA3.1(+) Laccase2 MCS Exon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541092
Proper citation: RRID:Addgene_126443 Copy
Species: Mus musculus
Genetic Insert: BMP-RIB
Vector Backbone Description: Backbone Size:6600; Vector Backbone:LPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12379805
Comments: LPCX was made by replacing the neo gene of LNCX with the puro gene.
Proper citation: RRID:Addgene_12642 Copy
Species: Mus musculus
Genetic Insert: m-Smoothened
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26291458
Proper citation: RRID:Addgene_126407 Copy
Species: Mus musculus
Genetic Insert: 2'3' cyclic nucleotide phosphodiesterase
Vector Backbone Description: Vector Backbone:PB-bsd; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30355738
Comments: Construct contains a non-canonical FLAG tag of DYKDDDDR rather than the typical DYKDDDDK that is functional in the experience of the depositor.
Proper citation: RRID:Addgene_126608 Copy
Species: Mus musculus
Genetic Insert: Lrig1
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high.
Homology arms subcloned from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Mice available from the MMRRC (https://www.mmrrc.org/catalog/sds.php?mmrrc_id=69516).
Proper citation: RRID:Addgene_126651 Copy
Species: Mus musculus
Genetic Insert: Ighg2b
Vector Backbone Description: Backbone Size:5729; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33268892
Comments: ENA accession: LR588430, https://www.ebi.ac.uk/ena/browser/view/LR588430
Proper citation: RRID:Addgene_127155 Copy
Species: Mus musculus
Genetic Insert: N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Entry vector with transgene for gateway cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30487607
Proper citation: RRID:Addgene_127177 Copy
Species: Mus musculus
Genetic Insert: Mouse Cdk12 Intron 3 sgRNA
Vector Backbone Description: Backbone Marker:Feng Zhang Lab (Addgene Plasmid #42230); Backbone Size:8484; Vector Backbone:pX330; Vector Types:Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30487607
Proper citation: RRID:Addgene_127174 Copy
Species: Mus musculus
Genetic Insert: Mouse Cyclin Dependent Kinase 12
Vector Backbone Description: Backbone Marker:Albert Cheng (Jackson Labs); Backbone Size:10369; Vector Backbone:PBNeo-TetO-Dest; Vector Types:Mammalian Expression, Piggybac Transposase Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30487607
Proper citation: RRID:Addgene_127178 Copy
Species: Mus musculus
Genetic Insert: Myc (NM_001177352)
Vector Backbone Description: Backbone Marker:Genecopoeia; Backbone Size:7450; Vector Backbone:pEZ-M56; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: based on published work for human
Proper citation: RRID:Addgene_127286 Copy
Species: Mus musculus
Genetic Insert: Myc (NM_001177352)
Vector Backbone Description: Backbone Marker:Genecopoeia; Backbone Size:7450; Vector Backbone:pEZ-M56; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: based on published work for human
Proper citation: RRID:Addgene_127285 Copy
Species: Mus musculus
Genetic Insert: ELL3 (NM_145973)
Vector Backbone Description: Backbone Marker:Genecopoeia; Backbone Size:8565; Vector Backbone:pEZ-Lv225; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_127283 Copy
Species: Mus musculus
Genetic Insert: ELL1 (NM_007924 plus CEYLHNK to AEYLHNK)
Vector Backbone Description: Backbone Marker:Genecopoeia; Backbone Size:8565; Vector Backbone:pEZ-Lv225; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Fails to degrade myc as published for human
Proper citation: RRID:Addgene_127279 Copy
Species: Mus musculus
Genetic Insert: Promoter+V-region of IgH of A20 B cell line
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18025212
Comments: The sequence is show in Figure 2 of the associated publication.
Proper citation: RRID:Addgene_127249 Copy
Species: Mus musculus
Genetic Insert: IgG2b gene with exons and alt pAs
Vector Backbone Description: Vector Backbone:pSV2gpt; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749764
Comments: Contains the whole 11 kb IgG2b gene from MPC11
Proper citation: RRID:Addgene_127248 Copy
Species: Mus musculus
Genetic Insert: mouse TREX1 cDNA
Vector Backbone Description: Backbone Size:13320; Vector Backbone:pTRIPZ RFP-; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28598415
Proper citation: RRID:Addgene_127701 Copy
Species: Mus musculus
Genetic Insert: shcGAS Mmu
Vector Backbone Description: Backbone Size:13320; Vector Backbone:pTRIPZ RFP+; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28598415
Proper citation: RRID:Addgene_127698 Copy
Species: Mus musculus
Genetic Insert: mSAN1
Vector Backbone Description: Backbone Marker:IBA Lifesciences; Backbone Size:3172; Vector Backbone:pASK-IBA3Plus; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29968717
Comments: MGC cDNA was a partial clone, repaired by the authors to generate full length mSAN1. mSAN1 contains a difference compared to NM_001357822.1 that results in a V549I protein-coding change.
Proper citation: RRID:Addgene_117165 Copy
Species: Mus musculus
Genetic Insert: Tet1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pDT; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_117147 Copy
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