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Species: Synthetic
Genetic Insert: NpuDnaE(C)-HygR
Vector Backbone Description: Vector Backbone:pGCS-N3(3xFLAG); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37237044
Proper citation: RRID:Addgene_205992 Copy
Species: Synthetic
Genetic Insert: ie1-EGFP
Vector Backbone Description: Vector Backbone:Janelia Atalanta cloning plasmid; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.17.545412v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_204301 Copy
Species: Synthetic
Genetic Insert: 3XP3-ECFP
Vector Backbone Description: Vector Backbone:Janelia Atalanta cloning plasmid; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_204302 Copy
Species: Synthetic
Genetic Insert: blasticidine - P2A - TETon-3G transactivator
Vector Backbone Description: Backbone Marker:Vlatka Zoldos; Backbone Size:8020; Vector Backbone:#71666 pdCas9-DNMT3A-EGFP; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36307419
Comments: A lentiviral UbC-blasticidine-P2A-TETon-3G transactivator vector was synthesized using the #71666 pdCas9-DNMT3A-EGFP vector backbone digested with NheI-EcoRI and ligated to the following DNA fragments: UbC promoter and blasticidine cassette amplified and fused by PCR using 40–50 bp internal overlapping primers and one external reverse primer containing a P2A linker, and a TETon-3G fragment amplified from the Addgene vector #96963 pCAG-TetON-3G using a forward primer containing a P2A sequence so it could be fused to the UbC-Blasticidin-P2A sequence during a last round PCR amplification with NheI and EcoRI external primers.
Proper citation: RRID:Addgene_200191 Copy
Species: Synthetic
Genetic Insert: MHC-EGFP+H3:H26
Vector Backbone Description: Vector Backbone:Janelia Atalanta cloning plasmid; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.17.545412v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_204289 Copy
Species: Synthetic
Genetic Insert: NbV5-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201477 Copy
Species: Synthetic
Genetic Insert: NbV5-eGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201473 Copy
Species: Synthetic
Genetic Insert: AsCas12f
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37400536
Proper citation: RRID:Addgene_204635 Copy
Species: Synthetic
Genetic Insert: NbALFA-TEV219
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201487 Copy
Species: Synthetic
Genetic Insert: trancated AsCas12f sgRNA (sgRNA-v2)
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37400536
Proper citation: RRID:Addgene_204639 Copy
Species: Synthetic
Genetic Insert: enAsCas12f
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37400536
Proper citation: RRID:Addgene_204637 Copy
Species: Synthetic
Genetic Insert: NbV5-TEV219
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5126; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201481 Copy
Species: Synthetic
Genetic Insert: LgBit-NbALFA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201484 Copy
Species: Synthetic
Genetic Insert: NbALFA-LgBit
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5502; Vector Backbone:pcDNA6/ V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201483 Copy
Species: Synthetic
Genetic Insert: GFP2-NbALFA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201486 Copy
Species: Synthetic
Genetic Insert: NbAlfa-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201485 Copy
Species: Synthetic
Genetic Insert: HiBiT-NbV5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201490 Copy
Species: Synthetic
Genetic Insert: jGCaMP7f
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34038743
Proper citation: RRID:Addgene_203848 Copy
Species: Synthetic
Genetic Insert: BCD 8.5K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200050 Copy
Species: Synthetic
Genetic Insert: BCD 0.45K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200055 Copy
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