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Species: Caenorhabditis elegans
Genetic Insert: unc-122 promoter
Vector Backbone Description: Backbone Size:4300; Vector Backbone:MC10-RFP; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10545240
Comments: Marker for expression of RFP in coelomocytes. 4kb unc-122 promoter+ 4aa of 1st exon (8911-12903 of cosmid F11C3, start is at 12892) in SphI-BamHI sites of MC10-RFP (dsRed in AgeI-NotI).
Proper citation: RRID:Addgene_8938 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-122 promoter
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10545240
Comments: Marker for expression of GFP in coelomocytes. 4kb unc-122 promoter+ 4aa of 1st exon (8911-12903 of cosmid F11C3, start is at 12892) in Pst-BamHI sites of pPD95.77.
Proper citation: RRID:Addgene_8937 Copy
Genetic Insert: GFP, Puromycin resistance
Vector Backbone Description: Vector Backbone:pMCB306, with addition of hU6 and BbsI sites for cloning of sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28319085
Comments: pKHH030 is derived from pMCB306, which was originally derived from pU6-sgGFP-NT1 (addgene #46914) from the Weissman and Qi labs
Proper citation: RRID:Addgene_89358 Copy
Species: Homo sapiens
Genetic Insert: MS2-P65-HSF1_2A_Hygro
Vector Backbone Description: Vector Backbone:plenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28333914
Proper citation: RRID:Addgene_89308 Copy
Species: Rattus norvegicus
Genetic Insert: rAPOBEC1-nCas9 (D10A)-UGI-NLS
Vector Backbone Description: Backbone Size:5379; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28244995
Proper citation: RRID:Addgene_89508 Copy
Species: Homo sapiens
Genetic Insert: ezrin siRNA
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pBS/U6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12769842
Comments: siRNA targeting ezrin. Sense strand of the hairpin is identical to a 22 nucleotide region in the target gene (ezrin [376-396]). Sequence is GGGTC CTACG CTGTG CAGGC CAAG.
Proper citation: RRID:Addgene_8945 Copy
Species: Mus musculus
Genetic Insert: PGC-1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14744933
Proper citation: RRID:Addgene_8943 Copy
Species: Synthetic
Genetic Insert: Asparaginyl endopeptidase from Oldenlandia affinis
Vector Backbone Description: Backbone Size:4540; Vector Backbone:pHYRSF53; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28369872
Proper citation: RRID:Addgene_89482 Copy
Vector Backbone Description: Vector Backbone:pMDC43; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23056524
Comments: To enhance folding of the fusion proteins a flexible linker was introduced between the coding sequence of either fragment of YFP and the attR recombination site. For more information see: http://www.ibmcp.upv.es/FerrandoLabVectors/
Proper citation: RRID:Addgene_89487 Copy
Genetic Insert: pMarC9-R6k
Vector Backbone Description: Backbone Marker:Victor de Lorenzo lab; Vector Backbone:pBAM1; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:30962569
Comments: BW29427 is an E. coli strain with diaminopimelic acid (DAP) auxotrophy. BW29427 growth requires 300uM DAP even when cultured in LB. Importantly, BW29427 does not grow in the absence of DAP, which simplifies counterselection of this host strain following biparental mating with Vibrio natriegens.
Please visit https://www.biorxiv.org/content/early/2016/06/12/058487 for bioRxiv preprint.
Proper citation: RRID:Addgene_89477 Copy
Genetic Insert: hairpin against mouse/human AMPKalpha2
Vector Backbone Description: Backbone Size:7900; Vector Backbone:LMP; Vector Types:Mammalian Expression, Mouse Targeting, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23274086
Proper citation: RRID:Addgene_89490 Copy
Species: Homo sapiens
Genetic Insert: L1CAM
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10469653
Proper citation: RRID:Addgene_89411 Copy
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28572162
Proper citation: RRID:Addgene_89392 Copy
Species: Danio rerio
Genetic Insert: crestin
Vector Backbone Description: Vector Backbone:bluescript; Vector Types:in situ probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10660676
Comments: To make antisense RNA probe for in situ hybridization cut with Sac I and use T7 polymerase
Proper citation: RRID:Addgene_89394 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FlpO
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28346402
Proper citation: RRID:Addgene_89574 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #11795; Backbone Size:10054; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27798563
Proper citation: RRID:Addgene_89638 Copy
Species: Homo sapiens
Genetic Insert: cyclin D1
Vector Backbone Description: Backbone Size:5542; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15684604
Comments: For parent plasmid information, see Author's Map link for Rc/CMV cyclin D1.
Proper citation: RRID:Addgene_8952 Copy
Species: Homo sapiens
Genetic Insert: VEGF-A(165)
Vector Backbone Description: Backbone Marker:Luigi Naldini, PMID 9765382; Backbone Size:8499; Vector Backbone:CCLc; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89609 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:plenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28416141
Proper citation: RRID:Addgene_89567 Copy
Species: phage lambda
Genetic Insert: 750 bp EcoRV fragment
Vector Backbone Description: Backbone Size:2635; Vector Backbone:pUC9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28550309
Proper citation: RRID:Addgene_89566 Copy
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