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Species: Synthetic
Genetic Insert: sgRNA cloning site
Vector Backbone Description: Backbone Marker:Addgene #60847; Vector Backbone:pCAS; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36287051
Comments: This plasmid is a modified version of pCAS (Addgene #60847) that has been improved to facilitate rapid cloning of new gRNA sequencing for streamlined CRISPR-Cas9 genome editing in yeast. pCAS was originally published by:
Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
Proper citation: RRID:Addgene_190175 Copy
Species: Homo sapiens
Genetic Insert: epoxide hydrolase 1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pcmv entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34342583
Proper citation: RRID:Addgene_169172 Copy
Species: Homo sapiens
Genetic Insert: epoxide hydrolase 1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pcmv entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34342583
Proper citation: RRID:Addgene_169173 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Víctor de Lorenzo; Backbone Size:3730; Vector Backbone:pSEVA221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36511859
Proper citation: RRID:Addgene_188965 Copy
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37930278
Comments: RFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
Proper citation: RRID:Addgene_180729 Copy
Species: Synthetic
Genetic Insert: 50%GC-40bp-hairpin-binding-site-reversed
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pCR-XL-2-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169198 Copy
Species: Synthetic
Genetic Insert: 66%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169199 Copy
Species: Synthetic
Genetic Insert: 50%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169196 Copy
Species: Synthetic
Genetic Insert: 50%GC-40bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169197 Copy
Species: Synthetic
Genetic Insert: 50-0-50%GC-Right-half-120bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Since the 120nt hairpin cannot be cloned in it's entirety the two halves (Left & Right) are provided here.
Proper citation: RRID:Addgene_169207 Copy
Species: Synthetic
Genetic Insert: 100%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169203 Copy
Species: Synthetic
Genetic Insert: 50%GC-Right-half-120bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Since the 120nt hairpin cannot be cloned in it's entirety the two halves (Left & Right) are provided here.
Proper citation: RRID:Addgene_169205 Copy
Species: Synthetic
Genetic Insert: 75%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169201 Copy
Species: Synthetic
Genetic Insert: ABE7.10-SpCas9-NG
Vector Backbone Description: Backbone Marker:Atum; Backbone Size:2256; Vector Backbone:pD881-SR; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34520545
Proper citation: RRID:Addgene_170663 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35998606
Proper citation: RRID:Addgene_188970 Copy
Species: Mus musculus
Genetic Insert: talin1 head P229S
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35861643
Proper citation: RRID:Addgene_182997 Copy
Species: Mus musculus
Genetic Insert: talin1 head
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35861643
Proper citation: RRID:Addgene_182996 Copy
Species: Mus musculus
Genetic Insert: Talin 1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35861643
Proper citation: RRID:Addgene_182995 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase cAMP-activated catalytic subunit alpha (PKA)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pAJM.029; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_154048 Copy
Species: Streptomyces antibioticus
Genetic Insert: Tyrosinase MelA
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pAJM.029; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_154049 Copy
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