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Species: Synthetic
Genetic Insert: BCD 0.2K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200056 Copy
Species: Synthetic
Genetic Insert: Pong TPase LA T2A ORF1SC1 ONE
Vector Backbone Description: Backbone Marker:ABRC; Backbone Size:11648; Vector Backbone:pEarleyGate 100; Vector Types:Plant Expression, Binary; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37273575
Proper citation: RRID:Addgene_196137 Copy
Species: Synthetic
Genetic Insert: SNACS S785A
Vector Backbone Description: Vector Backbone:pH7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32463362
Proper citation: RRID:Addgene_203577 Copy
Species: Synthetic
Genetic Insert: SNACS
Vector Backbone Description: Vector Backbone:pH7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32463362
Proper citation: RRID:Addgene_203576 Copy
Species: Synthetic
Genetic Insert: MCD8 weak RBS for assembly with tag part
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204105 Copy
Species: Synthetic
Genetic Insert: MCD15 medium RBS for assembly with tag part
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204103 Copy
Species: Synthetic
Genetic Insert: MCD7 medium RBS for assembly with tag part
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204102 Copy
Species: Synthetic
Genetic Insert: luxR-PLuxB repressor and PLuxB 3OC6-AHL-inducible promoter
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204073 Copy
Species: Synthetic
Genetic Insert: cymR repressor and PCymRC cumic acid-inducible promoter
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204071 Copy
Species: Synthetic
Genetic Insert: L3S2P21 strong terminator
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204116 Copy
Species: Synthetic
Genetic Insert: vanR repressor and PVanCC vanillic acid-inducible promoter
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204078 Copy
Species: Synthetic
Genetic Insert: T7 terminator
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204124 Copy
Species: Synthetic
Genetic Insert: MCD1 strong RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204091 Copy
Species: Synthetic
Genetic Insert: MCD7 medium RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204094 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Backbone Marker:addgene; Backbone Size:4915; Vector Backbone:pAAV-mDlx-dio; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38086373
Comments: 11bp sequence missing in AAV2 ITR sequence
Proper citation: RRID:Addgene_204689 Copy
Species: Synthetic
Genetic Insert: dummy 20nt sequence
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37682951
Proper citation: RRID:Addgene_207904 Copy
Species: Synthetic
Genetic Insert: dummy 20nt sequence
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37682951
Proper citation: RRID:Addgene_207905 Copy
Species: Synthetic
Genetic Insert: dummy 20nt sequence
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37682951
Proper citation: RRID:Addgene_207907 Copy
Species: Synthetic
Genetic Insert: dummy 20nt sequence
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37682951
Proper citation: RRID:Addgene_207900 Copy
Species: Synthetic
Genetic Insert: dummy 20nt sequence
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37682951
Proper citation: RRID:Addgene_207901 Copy
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