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Species: Streptococcus pyogenes
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:4897; Vector Backbone:n/a; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36995630
Comments: User inserts own guides for CRISPR in Medicago truncatula
Proper citation: RRID:Addgene_191768 Copy
Species: Homo sapiens
Genetic Insert: PRPF4
Vector Backbone Description: Backbone Size:6580; Vector Backbone:pET28GST-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: insert+fusion tag if applicable MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSSMGSSHHHHHHSSGLVPRGSMQELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWDLEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYHIATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE sequence of insert only (no fusion tag) MQELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWDLEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYHIATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE
Proper citation: RRID:Addgene_137650 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:pCAMBIA1305.1; Vector Types:Plant Expression, binary vector for plant transformation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34719678
Proper citation: RRID:Addgene_179835 Copy
Species: Synthetic
Genetic Insert: jGCaMP7c
Vector Backbone Description: Vector Backbone:pFR; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_191461 Copy
Species: C. intestinalis (ascidian)
Genetic Insert: Brachury notochord enhancer
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:2544; Vector Backbone:pDONR-221-P3-P4; Vector Types:Protein expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17878951
Comments: The Ciona Brachyury enhancer drives expression in the early notochord lineages.
Proper citation: RRID:Addgene_186368 Copy
Species: C. intestinalis (ascidian)
Genetic Insert: Titf endoderm enhancer
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:2544; Vector Backbone:pDONR-221-P3-P4; Vector Types:Protein expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17878951
Comments: The Titf1 cis-regulatory region drives expression in the early gastrula endoderm lineage.
Proper citation: RRID:Addgene_186367 Copy
Species: C. intestinalis (ascidian)
Genetic Insert: Snail regulatory sequence
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:2550; Vector Backbone:pDONR-221-P3-P5; Vector Types:Protein expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17878951
Comments: The Ci-Snail cis-regulatory region drives expression in mesodermal cells.
Proper citation: RRID:Addgene_186365 Copy
Species: Mus musculus
Genetic Insert: Cdk2ap1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Nonviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34644528
Proper citation: RRID:Addgene_178033 Copy
Vector Backbone Description: Vector Backbone:pC2YB; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28860184
Proper citation: RRID:Addgene_190043 Copy
Species: Synthetic
Genetic Insert: none
Vector Backbone Description: Vector Backbone:pDRESS; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_191473 Copy
Species: Synthetic
Genetic Insert: CH-GECO2.1
Vector Backbone Description: Vector Backbone:pFR; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_191470 Copy
Species: Synthetic
Genetic Insert: nGFPMNASE
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9473618
Proper citation: RRID:Addgene_187826 Copy
Species: Bacillus subtilis
Genetic Insert: rok
Vector Backbone Description: Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36408910
Proper citation: RRID:Addgene_178195 Copy
Species: Renilla reniformis
Genetic Insert: Act2::FRT-OCS-FRT::Rluc
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pAGM4673; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35788565
Comments: Other genes: TCTP1::Fluc, 35S::FlpO
Proper citation: RRID:Addgene_192381 Copy
Species: Bacillus subtilis
Genetic Insert: rok 6xHis
Vector Backbone Description: Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36408910
Proper citation: RRID:Addgene_178198 Copy
Species: Bacillus subtilis
Genetic Insert: srok
Vector Backbone Description: Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36408910
Proper citation: RRID:Addgene_178196 Copy
Species: Renilla reniformis
Genetic Insert: Act2::B3RT-OCS-B3RT::Rluc
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pAGM4673; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35788565
Comments: Other genes: TCTP1::Fluc, TCTP1::B3o
Proper citation: RRID:Addgene_192384 Copy
Species: Renilla reniformis
Genetic Insert: Act2::B3RT-OCS-B3RT::Rluc
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pAGM4673; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35788565
Comments: Other genes: TCTP1::Fluc
Proper citation: RRID:Addgene_192383 Copy
Species: Renilla reniformis
Genetic Insert: B3RT-Act2-B3RT::Rluc
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pAGM4673; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35788565
Comments: Other genes: TCTP1::Fluc, TCTP1::B3o
Proper citation: RRID:Addgene_192405 Copy
Species: Renilla reniformis
Genetic Insert: FRT-Act2-FRT::Rluc
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pAGM4673; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35788565
Comments: Other genes: TCTP1::Fluc
Proper citation: RRID:Addgene_192402 Copy
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