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pCASB
 
Resource Report
Resource Website 		RRID:Addgene_190175 sgRNA cloning site Synthetic Kanamycin PMID:36287051 This plasmid is a modified version of pCAS (Addgene #60847) that has been improved to facilitate rapid cloning of new gRNA sequencing for streamlined CRISPR-Cas9 genome editing in yeast. pCAS was originally published by: Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703. Backbone Marker:Addgene #60847; Vector Backbone:pCAS; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin Added 5'-agagacc-3' upstream and 5'-ggtctca-3' downstream of the gRNA site to allow for golden gate cloning of new gRNAs with the restriction enzyme Bsa1 2026-08-15 01:23:58 0
pCMV6-Entry-EPHX1c.1212G>C-Flag
 
Resource Report
Resource Website 		RRID:Addgene_169172 epoxide hydrolase 1 Homo sapiens Kanamycin PMID:34342583 Backbone Size:4900; Vector Backbone:pcmv entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin c.1212G>C 2026-08-15 01:23:55 0
pCMV6-Entry-EPHX1c.1288G>C-Flag
 
Resource Report
Resource Website 		RRID:Addgene_169173 epoxide hydrolase 1 Homo sapiens Kanamycin PMID:34342583 Backbone Size:4900; Vector Backbone:pcmv entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin c.1288G>C 2026-08-15 01:23:55 0
pSLiP-G1G2
 
Resource Report
Resource Website 		RRID:Addgene_188965 dCas9 Synthetic Kanamycin PMID:36511859 Backbone Marker:Víctor de Lorenzo; Backbone Size:3730; Vector Backbone:pSEVA221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:24:00 0
pMYT076
 
Resource Report
Resource Website 		RRID:Addgene_180729 Kanamycin PMID:37930278 RFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time. Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:23:57 0
hp-50%GC-40bp-Revbindsite
 
Resource Report
Resource Website 		RRID:Addgene_169198 50%GC-40bp-hairpin-binding-site-reversed Synthetic Kanamycin PMID:36063324 Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region. Backbone Size:3956; Vector Backbone:pCR-XL-2-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin 2026-08-15 01:23:56 0
hp-66%GC-20bp
 
Resource Report
Resource Website 		RRID:Addgene_169199 66%GC-20bp-hairpin Synthetic Kanamycin PMID:36063324 Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region. Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin 2026-08-15 01:23:56 0
hp-50%GC-20bp
 
Resource Report
Resource Website 		RRID:Addgene_169196 50%GC-20bp-hairpin Synthetic Kanamycin PMID:36063324 Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region. Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin 2026-08-15 01:23:56 0
hp-50%GC-40bp
 
Resource Report
Resource Website 		RRID:Addgene_169197 50%GC-40bp-hairpin Synthetic Kanamycin PMID:36063324 Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region. Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin 2026-08-15 01:23:59 0
hp-50-0-50%GC-Right-half-120bp
 
Resource Report
Resource Website 		RRID:Addgene_169207 50-0-50%GC-Right-half-120bp-hairpin Synthetic Kanamycin PMID:36063324 Since the 120nt hairpin cannot be cloned in it's entirety the two halves (Left & Right) are provided here. Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin 2026-08-15 01:23:56 0
hp-100%GC-20bp
 
Resource Report
Resource Website 		RRID:Addgene_169203 100%GC-20bp-hairpin Synthetic Kanamycin PMID:36063324 Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region. Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin 2026-08-15 01:23:59 0
hp-50%GC-Right-half-120bp
 
Resource Report
Resource Website 		RRID:Addgene_169205 50%GC-Right-half-120bp-hairpin Synthetic Kanamycin PMID:36063324 Since the 120nt hairpin cannot be cloned in it's entirety the two halves (Left & Right) are provided here. Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin 2026-08-15 01:23:56 0
hp-75%GC-20bp
 
Resource Report
Resource Website 		RRID:Addgene_169201 75%GC-20bp-hairpin Synthetic Kanamycin PMID:36063324 Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region. Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin 2026-08-15 01:23:56 0
pRha-ABE7.10-SpCas9-NG
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_170663 ABE7.10-SpCas9-NG Synthetic Kanamycin PMID:34520545 Backbone Marker:Atum; Backbone Size:2256; Vector Backbone:pD881-SR; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:57 1
pREDusk-MCS
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_188970 Kanamycin PMID:35998606 Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:24:14 1
talin1-head P229S
 
Resource Report
Resource Website 		RRID:Addgene_182997 talin1 head P229S Mus musculus Kanamycin PMID:35861643 Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin P229S 2026-08-15 01:24:13 0
talin1-head
 
Resource Report
Resource Website 		RRID:Addgene_182996 talin1 head Mus musculus Kanamycin PMID:35861643 Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:24:13 0
mini-talin P229S (headR13DD P229S)
 
Resource Report
Resource Website 		RRID:Addgene_182995 Talin 1 Mus musculus Kanamycin PMID:35861643 Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin P229S 2026-08-15 01:24:13 0
enz.099-PKA
 
Resource Report
Resource Website 		RRID:Addgene_154048 Protein kinase cAMP-activated catalytic subunit alpha (PKA) Homo sapiens Kanamycin Backbone Size:2958; Vector Backbone:pAJM.029; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:24:15 0
enz.105-MelA
 
Resource Report
Resource Website 		RRID:Addgene_154049 Tyrosinase MelA Streptomyces antibioticus Kanamycin Backbone Size:2958; Vector Backbone:pAJM.029; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:24:15 0

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