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Species: Synthetic
Genetic Insert: OptoJNKi
Vector Backbone Description: Backbone Marker:Derived by our lab using pExFP-C1 and pGL3; Backbone Size:5673; Vector Backbone:pLuc-C1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28497795
Comments: Please see
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017 in press.
Proper citation: RRID:Addgene_89744 Copy
Genetic Insert: ZFN-AAVS1
Vector Backbone Description: Vector Backbone:pCMVpA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29099045
Proper citation: RRID:Addgene_89707 Copy
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28346402
Proper citation: RRID:Addgene_89689 Copy
Species: Homo sapiens
Genetic Insert: MEF2C
Vector Backbone Description: Backbone Marker:Scott Armstrong; Backbone Size:6957; Vector Backbone:MSCV-IRES-Tomato; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Proper citation: RRID:Addgene_89715 Copy
Species: P. buccae ATCC 33574
Genetic Insert: Cas13b
Vector Backbone Description: Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28065598
Comments: Benchling sequence link: https://benchling.com/s/hqikFD6s
Proper citation: RRID:Addgene_89906 Copy
Species: Synthetic
Genetic Insert: 6X CArG-RE
Vector Backbone Description: Vector Backbone:pCSC-G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27350449
Comments: CArG response element inserted upstream upstream of the ZsGreen fluorescent reporter; also expresses DsRedExpress2
Information can also be found in Moharil, et al. PLoS One. 2015 Oct 27;10(10):e0141365. doi: 10.1371 https://www.ncbi.nlm.nih.gov/pubmed/26505747 .
Proper citation: RRID:Addgene_89762 Copy
Species: Homo sapiens
Genetic Insert: Histone H2B
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:7000; Vector Backbone:pLenti6/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28232523
Proper citation: RRID:Addgene_89766 Copy
Species: Synthetic
Genetic Insert: TM-CIBN-TevN-BLITz1-TetR-VP16
Vector Backbone Description: Vector Backbone:pSyc 181 P2A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Proper citation: RRID:Addgene_89878 Copy
Species: Synthetic
Genetic Insert: bArrestin2-TevC-P2A-TdTomato
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Comments: Please note that mutation R173H in TdTomato was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_89873 Copy
Species: Synthetic
Genetic Insert: tetO-EGFP
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Proper citation: RRID:Addgene_89875 Copy
Species: Synthetic
Genetic Insert: DRD2-V2tail-TevN-BLITz1-TetR-VP16
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Proper citation: RRID:Addgene_89874 Copy
Species: Homo sapiens
Genetic Insert: Orai1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6100; Vector Backbone:pLNCX2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25404747
Comments: Addgene's sequencing results found an E275D mutation in Orai1 compared to NCBI reference sequence NP_116179.2. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_89818 Copy
Species: Homo sapiens
Genetic Insert: STIM1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6100; Vector Backbone:pLNCX2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25404747
Proper citation: RRID:Addgene_89817 Copy
Species: Homo sapiens
Genetic Insert: STIM1 shRNA
Vector Backbone Description: Backbone Marker:oligoengine; Backbone Size:4400; Vector Backbone:pSUPER-puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25404747
Proper citation: RRID:Addgene_89816 Copy
Species: Danio rerio
Genetic Insert: HA-BirA-2A-citrine
Vector Backbone Description: Backbone Size:4780; Vector Backbone:pGEM-GFP-SV40pA-FRT-Kan-FRT; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29666124
Proper citation: RRID:Addgene_89890 Copy
Species: Synthetic
Genetic Insert: site-specific DNA-methyltransferase SssI
Vector Backbone Description: Backbone Size:14759; Vector Backbone:pLV hUbC-dCas9-T2A-GFP (#53191); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28695892
Proper citation: RRID:Addgene_89793 Copy
Species: Synthetic
Genetic Insert: LSSmCherry1
Vector Backbone Description: Vector Backbone:pBAD His B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28241009
Proper citation: RRID:Addgene_89986 Copy
Species: Homo sapiens
Genetic Insert: SOX2-T2A-2xNLS-TdTomato-F2A-Puro
Vector Backbone Description: Backbone Size:2657; Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28952927
Proper citation: RRID:Addgene_89991 Copy
Species: Homo sapiens
Genetic Insert: human syntaxin17 lacking N-terminal domain
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6875; Vector Backbone:pMRXIP GFP-Ci2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28598244
Proper citation: RRID:Addgene_89941 Copy
Species: Rattus norvegicus
Genetic Insert: rat Lamp1
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6832; Vector Backbone:pMRXIP Venus-N; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27885029
Proper citation: RRID:Addgene_89937 Copy
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