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Genetic Insert: FKBP-linker-Myc-NBirA*
Vector Backbone Description: Vector Backbone:pSF3; Vector Types:Mammalian Expression, Retroviral, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28585547
Proper citation: RRID:Addgene_90009 Copy
Species: Homo sapiens
Genetic Insert: BRD4
Vector Backbone Description: Backbone Size:5137; Vector Backbone:pcDNA5 frt/to; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26735014
Proper citation: RRID:Addgene_90005 Copy
Species: Ramazzottius varieornatus
Genetic Insert: RvLEAM
Vector Backbone Description: Backbone Marker:Modified from pET-22b(+) [Park J-M, et al (2002) Biochem Biophys Res Commun 291, 23-28.]; Vector Backbone:pEThT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25675104
Comments: Insert species is Ramazzottius varieornatus
Proper citation: RRID:Addgene_90033 Copy
Species: S. pyogenes
Genetic Insert: Cas9HF1
Vector Backbone Description: Vector Backbone:pCas9-CR4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28915012
Comments: This Cas9 has been modified to contain the high-fidelity mutations for SpCas9-HF1 from Kleinstiver et al 2016
Proper citation: RRID:Addgene_89961 Copy
Species: S. pyogenes
Genetic Insert: Cas9HF1
Vector Backbone Description: Vector Backbone:pEM-Cas9HF1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28915012
Proper citation: RRID:Addgene_89962 Copy
Genetic Insert: trmI gRNA
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28915012
Proper citation: RRID:Addgene_89955 Copy
Species: Ramazzottius varieornatus
Genetic Insert: Dsup
Vector Backbone Description: Backbone Marker:Takara ; Vector Backbone:pCold-I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27649274
Comments: Insert species is Ramazzottius varieornatus
Proper citation: RRID:Addgene_90021 Copy
Species: Mus musculus
Genetic Insert: F-actin-capping protein subunit alpha-1
Vector Backbone Description: Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26564775
Proper citation: RRID:Addgene_89950 Copy
Species: E. coli
Genetic Insert: LacI-11/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, stop codon inserted 11 amino acids from 3'-end of the gene; polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90059 Copy
Species: E. coli
Genetic Insert: LacI/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90058 Copy
Genetic Insert: CFP
Vector Backbone Description: Backbone Size:9449; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression, Broad-host range; Bacterial Resistance:Gentamicin
Defining Citation: PMID:20522779
Proper citation: RRID:Addgene_90103 Copy
Genetic Insert: YcgR
Vector Backbone Description: Backbone Size:9449; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression, Broad-host range; Bacterial Resistance:Gentamicin
Defining Citation: PMID:20522779
Proper citation: RRID:Addgene_90102 Copy
Species: E. coli
Genetic Insert: FruR (CRA)
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: WT FruR (CRA) . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90066 Copy
Species: Synthetic
Genetic Insert: LLhE_AW
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: E. coli LacI DNA binding domain, LacI linker region, and T. fusca CelR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90062 Copy
Species: Synthetic
Genetic Insert: DEL
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: LLhG coding region on pHG165a interrupted with a single base-pair deletion . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90064 Copy
Species: Francisella tularensis subsp. novicida U112
Genetic Insert: FnCpf1 (humanized)
Vector Backbone Description: Backbone Size:6643; Vector Backbone:pET-28; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26422227
Comments: Note: a Flag-AviTag containing fragment follows the insert (between the XhoI and HindIII sites) but this sequence is not translated.
Proper citation: RRID:Addgene_90094 Copy
Species: Homo sapiens
Genetic Insert: Akt1 K179M T308A S473A
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBabe puro L; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11782364
Proper citation: RRID:Addgene_9013 Copy
Species: Homo sapiens
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10051603
Comments: The sequence of the HA tag in this plasmid is YAYDVPDYA instead of YPYDVPDYA. Not all HA antibodies will recognize this sequence. Cell Signaling Technology's HA antibody #2367 will work.
Proper citation: RRID:Addgene_9008 Copy
Species: Homo sapiens
Genetic Insert: Akt1 K179M
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10051603
Comments: An Akt-1 cDNA was amplified by reverse transcription-coupled PCR from total HeLa cell RNA and reamplified with a 5' primer containing a Kozak sequence and sequences encoding a hemagglutinin (HA) epitope and cloned into pLNCX to give pLNCX-HA-Akt. A double-stranded oligonucleotide encoding the src myristoylation sequence was inserted 5' of the HA tag to generate pLNCX-Myr-HA-Akt. K179M mutation generated using site-directed mutagenesis.
Proper citation: RRID:Addgene_9006 Copy
Species: Homo sapiens
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10051603
Comments: An Akt-1 cDNA was amplified by reverse transcription-coupled PCR from total HeLa cell RNA and reamplified with a 5' primer containing a Kozak sequence and sequences encoding a hemagglutinin (HA) epitope and cloned into pLNCX to give pLNCX-HA-Akt. A double-stranded oligonucleotide encoding the src myristoylation sequence was inserted 5' of the HA tag to generate pLNCX-Myr-HA-Akt.
Proper citation: RRID:Addgene_9005 Copy
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