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Species: Synthetic
Genetic Insert: BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
Vector Backbone Description: Vector Backbone:piggyBac-EF1alpha; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34403072
Comments: Plasmid name in Yang lab: HGpl268
Proper citation: RRID:Addgene_176461 Copy
Species: Ktedonobacter racemifer
Genetic Insert: non-coding locus components from KraIscB-1 locus
Vector Backbone Description: Backbone Size:3719; Vector Backbone:pCOLADuet1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34591643
Proper citation: RRID:Addgene_176537 Copy
Species: Allochromatium warmingii, Brachypodium distachyon
Genetic Insert: AwaIscB
Vector Backbone Description: Backbone Size:5062; Vector Backbone:pET45b(+); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591643
Proper citation: RRID:Addgene_176539 Copy
Genetic Insert: puroR-T2A-TetOn3G
Vector Backbone Description: Vector Backbone:pCamR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29507340
Comments: Addgene QC identified a P93S difference in the PuroR selectable marker that the depositing lab does not believe to affect function.
Proper citation: RRID:Addgene_176496 Copy
Species: Homo sapiens
Genetic Insert: RBBP7
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
Comments: SGC link: http://www.thesgc.org/structures/7M3X/.
Proper citation: RRID:Addgene_176411 Copy
Species: E. coli, Brachypodium distachyon, Delaware Bay metagenome
Genetic Insert: CRISPR-associated IscB (Delaware Bay metagenome)
Vector Backbone Description: Backbone Size:5062; Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591643
Proper citation: RRID:Addgene_176534 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mitochondrial FAD-linked sulfhydryl oxidase
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176405 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Backbone Size:598; Vector Backbone:LivePAR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34731617
Proper citation: RRID:Addgene_176526 Copy
Species: Other
Genetic Insert: FAD-linked sulfhydryl oxidase ERV2 from Fol
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176404 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Backbone Marker:Addgene 171992; Vector Backbone:PEA1-Nuclease-Puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Proper citation: RRID:Addgene_176528 Copy
Species: Homo sapiens
Genetic Insert: Nurr1
Vector Backbone Description: Backbone Size:11000; Vector Backbone:Piggybac TetO - Blastocidin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34171286
Proper citation: RRID:Addgene_176485 Copy
Species: Homo sapiens
Genetic Insert: Lmx1A-T2A-Pitx3
Vector Backbone Description: Backbone Size:6585; Vector Backbone:Piggybac TetO - puromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34171286
Proper citation: RRID:Addgene_176484 Copy
Species: Mus musculus
Genetic Insert: mouse Wnt1 cDNA
Vector Backbone Description: Backbone Size:8393; Vector Backbone:TetO-FUW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34171286
Proper citation: RRID:Addgene_176486 Copy
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2194165
Comments: Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.
If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.
Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning.
Proper citation: RRID:Addgene_1765 Copy
Species: Homo sapiens
Genetic Insert: En1-t2a-FoxA2
Vector Backbone Description: Backbone Size:8621; Vector Backbone:Piggybac TetO - hygromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34171286
Proper citation: RRID:Addgene_176483 Copy
Genetic Insert: SARS-CoV-2-NTD-B.1.618
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176438 Copy
Genetic Insert: SARS-CoV-2-RBD-N501Y
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176439 Copy
Genetic Insert: SARS-CoV-2-NTD-W152C
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176431 Copy
Genetic Insert: SARS-CoV-2-NTD-G142D-E154K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176434 Copy
Genetic Insert: SARS-CoV-2-NTD-E154K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176433 Copy
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