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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pJET-59-DExCon-mTagBFP2-R25
 
Resource Report
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1+ mentions
RRID:Addgene_179900 RAB25, member RAS oncogene family Homo sapiens Ampicillin PMID:35708998 Here donor for DExCon Rab25 knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work. https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin 2026-08-15 01:23:03 1
pAAV-OTp-hM3Dq-Myc
 
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1+ mentions
RRID:Addgene_184753 hM3D (Gq) Homo sapiens Ampicillin PMID:35443152 Backbone Marker:n/a; Backbone Size:2900; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:23:02 1
pCDH-AtAFB2-mTagBFP2
 
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1+ mentions
RRID:Addgene_179889 AtAFB2 Arabidopsis thaliana Ampicillin PMID:35708998 This lentiviral plasmids delivers AtAFB2 (auxin-inducible degron system) fused to mTagBFP2. In combination with TetOn3G can be used to generate DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:Systembio; Backbone Size:6390; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:23:02 1
pCDH-EF1a-HygroR-T2A-TetOn3G
 
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1+ mentions
RRID:Addgene_179887 TetOn3G Synthetic Ampicillin PMID:35708998 Lentiviral vector encoding Tet-On 3G constitutively expressed by EF1a promoter. Inducible promoter TRE3GS has to be delivered separately (e.g. DExCon knock in = Doxycycline-mediated endogenous gene Expression Control) https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281 Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:Systembio; Backbone Size:6370; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:23:02 1
pCDH-EF1a-mTagBFP-T2A-TetOn3G
 
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1+ mentions
RRID:Addgene_179888 TetOn3G Ampicillin PMID:35708998 Lentiviral vector encoding Tet-On 3G constitutively expressed by EF1a promoter. Inducible promoter TRE3GS has to be delivered separately (e.g. DExCon knock in = Doxycycline-mediated endogenous gene Expression Control) https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:Systembio; Backbone Size:6370; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:23:03 2
pAAV-CAG-LiLac
 
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1+ mentions
RRID:Addgene_184570 LiLac fluorescent sensor of lactate Synthetic Ampicillin PMID:35614105 mTurquoise2 sequences are originally from Goedhart, J. et al. Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat Commun 3, 751 (2012) and were obtained by PCR from the plasmid encoding the Lck-cpmTq2-Calcium-lifetime-sensor (Addgene plasmid #129627) (A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. van der Linden FH, Mahlandt EK, Arts JJG, Beumer J, Puschhof J, de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ Jr, Goedhart J. Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w.) Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:23:02 1
pMKRQ_11_DExCon-mNeonGreen-R25
 
Resource Report
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1+ mentions
RRID:Addgene_179896 RAB25, member RAS oncogene family Homo sapiens Kanamycin PMID:35708998 Here donor for DExCon Rab25 knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work. https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:GeneArt/Life Technologies; Backbone Size:3000; Vector Backbone:pMK-RQ; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Kanamycin 2026-08-15 01:23:03 1
pJET-22-DExCon-mNeonGreen-R11a
 
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1+ mentions
RRID:Addgene_179897 RAB11A, member RAS oncogene family Homo sapiens Ampicillin PMID:35708998 Here donor for DExCon Rab11a knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work. https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin 2026-08-15 01:23:03 1
pJET-64-SFFV-T2A-BlaR-polyA-R11b
 
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1+ mentions
RRID:Addgene_179895 RAB11B, member RAS oncogene family Homo sapiens Ampicillin PMID:35708998 Here donor for integration of SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out) to Rab11b locus This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11b expression (from the second allele) on demand DExCon = Doxycycline-mediated endogenous gene Expression Control https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin 2026-08-15 01:23:03 1
pJET-23-DExCon-mCherry-R11b
 
Resource Report
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1+ mentions
RRID:Addgene_179898 RAB11B, member RAS oncogene family Homo sapiens Ampicillin PMID:35708998 Here donor for DExCon mCherry Rab11b knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn3G is also required for this to work. https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin 2026-08-15 01:23:03 1
pJET-67-DExCon-mNeonGreen-R11b
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_179899 RAB11B, member RAS oncogene family Homo sapiens Ampicillin PMID:35708998 Here donor for DExCon mNeonGreen Rab11b knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn3G is also required for this to work. https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin 2026-08-15 01:23:03 1
pJET-62-SFFV-T2A-PuroR-polyA-R11b
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_179893 RAB11B, member RAS oncogene family Homo sapiens Ampicillin PMID:35708998 Here donor for integration of SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out) to Rab11b locus This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11b expression (from the second allele) on demand DExCon = Doxycycline-mediated endogenous gene Expression Control https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281. Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint. Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin 2026-08-15 01:23:02 1
L4440_BioBrick-SCR-sgRNA-B
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_177812 Scrambled sgRNA targeting sequences E. coli Ampicillin PMID:35636511 Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:03 1
pCJH027_SC101_pLAC_LbCas12a_Kan
 
Resource Report
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1+ mentions
RRID:Addgene_183074 LbCas12a Lachnospiraceae bacterium Kanamycin PMID:35659325 The sequence of LbCas12a was from Addgene plasmid # 113431 ; http://n2t.net/addgene:113431 ; RRID:Addgene_113431 Vector Backbone:pSC101 ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Wild-type 2026-08-15 01:23:04 1
pSEM246 - MCS mosTI unc-119 target
 
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1+ mentions
RRID:Addgene_159821 MCS mosTI unc-119 target Caenorhabditis elegans Ampicillin PMID:35900171 See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint. Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin Not applicable 2026-08-15 01:23:03 1
pY108 (lenti-AsCpf1)
 
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10+ mentions
RRID:Addgene_84739 huAsCpf1 Synthetic Ampicillin PMID:27918548 Contains a BsmBI cloning cassette for insertion of Cpf1 guides under U6 promoter control. Backbone Size:8069; Vector Backbone:pHKO_23; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:23:04 12
pJL1
 
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1+ mentions
RRID:Addgene_184129 MRP-1 Caenorhabditis elegans Ampicillin PMID:35513700 Backbone Marker:Fire Lab; Backbone Size:4702; Vector Backbone:pPD95.77_SL2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:05 1
pJL6
 
Resource Report
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1+ mentions
RRID:Addgene_184134 CELE_VW02B12L.1 Caenorhabditis elegans Ampicillin PMID:35513700 Backbone Marker:Piali Sengupta Lab; Backbone Size:4194; Vector Backbone:coel::rfp; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:05 1
pJL3
 
Resource Report
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1+ mentions
RRID:Addgene_184131 MRP-1 Caenorhabditis elegans Ampicillin PMID:35513700 Backbone Marker:Fire Lab; Backbone Size:4376; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:05 1
pLV-mScarlet-Control-5'UTR
 
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1+ mentions
RRID:Addgene_184637 mScarlet Other Ampicillin PMID:35149555 Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:23:04 1

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