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Species: Homo sapiens
Genetic Insert: RAB25, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab25 knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179900 Copy
Species: Homo sapiens
Genetic Insert: hM3D (Gq)
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:2900; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35443152
Proper citation: RRID:Addgene_184753 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtAFB2
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6390; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: This lentiviral plasmids delivers AtAFB2 (auxin-inducible degron system) fused to mTagBFP2. In combination with TetOn3G can be used to generate DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179889 Copy
Species: Synthetic
Genetic Insert: TetOn3G
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6370; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Lentiviral vector encoding Tet-On 3G constitutively expressed by EF1a promoter. Inducible promoter TRE3GS has to be delivered separately (e.g. DExCon knock in = Doxycycline-mediated endogenous gene Expression Control)
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179887 Copy
Genetic Insert: TetOn3G
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6370; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Lentiviral vector encoding Tet-On 3G constitutively expressed by EF1a promoter. Inducible promoter TRE3GS has to be delivered separately (e.g. DExCon knock in = Doxycycline-mediated endogenous gene Expression Control)
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179888 Copy
Species: Synthetic
Genetic Insert: LiLac fluorescent sensor of lactate
Vector Backbone Description: Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35614105
Comments: mTurquoise2 sequences are originally from Goedhart, J. et al. Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat Commun 3, 751 (2012) and were obtained by PCR from the plasmid encoding the Lck-cpmTq2-Calcium-lifetime-sensor (Addgene plasmid #129627) (A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. van der Linden FH, Mahlandt EK, Arts JJG, Beumer J, Puschhof J, de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ Jr, Goedhart J. Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w.)
Proper citation: RRID:Addgene_184570 Copy
Species: Homo sapiens
Genetic Insert: RAB25, member RAS oncogene family
Vector Backbone Description: Backbone Marker:GeneArt/Life Technologies; Backbone Size:3000; Vector Backbone:pMK-RQ; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab25 knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179896 Copy
Species: Homo sapiens
Genetic Insert: RAB11A, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab11a knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179897 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for integration of SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out) to Rab11b locus
This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11b expression (from the second allele) on demand
DExCon = Doxycycline-mediated endogenous gene Expression Control
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179895 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon mCherry Rab11b knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179898 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon mNeonGreen Rab11b knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179899 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for integration of SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out) to Rab11b locus
This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11b expression (from the second allele) on demand
DExCon = Doxycycline-mediated endogenous gene Expression Control
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179893 Copy
Species: E. coli
Genetic Insert: Scrambled sgRNA targeting sequences
Vector Backbone Description: Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35636511
Proper citation: RRID:Addgene_177812 Copy
Species: Lachnospiraceae bacterium
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pSC101 ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35659325
Comments: The sequence of LbCas12a was from Addgene plasmid # 113431 ; http://n2t.net/addgene:113431 ; RRID:Addgene_113431
Proper citation: RRID:Addgene_183074 Copy
Species: Caenorhabditis elegans
Genetic Insert: MCS mosTI unc-119 target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159821 Copy
Species: Synthetic
Genetic Insert: huAsCpf1
Vector Backbone Description: Backbone Size:8069; Vector Backbone:pHKO_23; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27918548
Comments: Contains a BsmBI cloning cassette for insertion of Cpf1 guides under U6 promoter control.
Proper citation: RRID:Addgene_84739 Copy
Species: Caenorhabditis elegans
Genetic Insert: MRP-1
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:4702; Vector Backbone:pPD95.77_SL2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35513700
Proper citation: RRID:Addgene_184129 Copy
Species: Caenorhabditis elegans
Genetic Insert: CELE_VW02B12L.1
Vector Backbone Description: Backbone Marker:Piali Sengupta Lab; Backbone Size:4194; Vector Backbone:coel::rfp; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35513700
Proper citation: RRID:Addgene_184134 Copy
Species: Caenorhabditis elegans
Genetic Insert: MRP-1
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:4376; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35513700
Proper citation: RRID:Addgene_184131 Copy
Species: Other
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35149555
Proper citation: RRID:Addgene_184637 Copy
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