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Species: Homo sapiens
Genetic Insert: RAF1
Vector Backbone Description: Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_185371 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA cassette under U3 promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35022409
Comments: This vector was designed and constructed by Dr. Tomasz Bieluszewski.
Detailed protocol for CRISPR/Cas9-based mutagenesis using this system in:
Bieluszewski T, Szymanska-Lejman M, Dziegielewski W, Zhu L, Ziolkowski PA. Efficient Generation of CRISPR/Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions, in: Plant Gametogenesis: Methods and Protocols (ed. Christophe Lambing), Methods in Molecular Biology, vol. 2484.
https://doi.org/10.1007/978-1-0716-2253-7_13
https://pubmed.ncbi.nlm.nih.gov/35461452/
Proper citation: RRID:Addgene_173156 Copy
Species: Homo sapiens
Genetic Insert: NDUFAF5
Vector Backbone Description: Vector Backbone:pMXs-IRES-Bsd; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28162770
Proper citation: RRID:Addgene_86125 Copy
Species: Homo sapiens
Genetic Insert: Plexin-B2
Vector Backbone Description: Backbone Marker:Feng Zhang laboratory, MIT; Backbone Size:12992; Vector Backbone:plentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514835
Proper citation: RRID:Addgene_86152 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Feng Zhang laboratory, MIT; Backbone Size:12992; Vector Backbone:plentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514835
Comments: This lenti-CRISPRv2 vector was used as control for studies with gene specific lenti-CRISPRv2 gene deletion of Plexin-B2 in human cells (plentiCRISPRv2-sgPLXNB2).
Plasmid received from Jialiang Liang, Laboratory of Patrizia Casaccia, Icahn School of Medicine at Mount Sinai
Proper citation: RRID:Addgene_86153 Copy
Species: Arabidopsis thaliana
Genetic Insert: FLS2
Vector Backbone Description: Vector Backbone:pCAMBIA2300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16510871
Proper citation: RRID:Addgene_86157 Copy
Species: Homo sapiens
Genetic Insert: TIAM1
Vector Backbone Description: Vector Backbone:pMXs3-IRES-turboRFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28162770
Proper citation: RRID:Addgene_86143 Copy
Species: Homo sapiens
Genetic Insert: Plexin-B2
Vector Backbone Description: Backbone Marker:Kaufman laboratory, University of Massachusetts; Backbone Size:9778; Vector Backbone:pLENTI PGK Neo DEST ADDGENE #19067; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514835
Proper citation: RRID:Addgene_86237 Copy
Species: Mus musculus
Genetic Insert: AID[71-114]-eGFP
Vector Backbone Description: Vector Backbone:pFNF (Addgene #22687); Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28525758
Proper citation: RRID:Addgene_86230 Copy
Species: Lachnospiraceae bacterium ND2006
Genetic Insert: LbCpf1
Vector Backbone Description: Vector Backbone:pYPQ221; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28211909
Proper citation: RRID:Addgene_86211 Copy
Vector Backbone Description: Vector Backbone:pMDC32; Vector Types:Plant Expression, Gateway compatible attR1-attR2 destination vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:28211909
Comments: Note: Addgene's quality control sequencing finds a 1.2kb transposable element upstream of the Kanamycin resistance cassette. This inserted sequence does not affect plasmid function.
Proper citation: RRID:Addgene_86207 Copy
Species: Human papillomavirus
Genetic Insert: HPV-16 E6/E7
Vector Backbone Description: Backbone Size:7900; Vector Backbone:p1318; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2476573
Comments: See author's map.
Proper citation: RRID:Addgene_8641 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NOP1pr-GFP11-mCherry-Scs2TM
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27831485
Proper citation: RRID:Addgene_86416 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NOP1pr-GFP11-PUS1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27831485
Proper citation: RRID:Addgene_86414 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GFP1-10
Vector Backbone Description: Backbone Size:8200; Vector Backbone:pFA6-link-CaURA3MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27831485
Comments: for C-terminal PCR tagging with GFP1-10
Proper citation: RRID:Addgene_86419 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NOP1pr-mCherry-SCS2TM-GFP11
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27831485
Proper citation: RRID:Addgene_86417 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NOP1pr-GFP1-10-Scs2TM
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27831485
Proper citation: RRID:Addgene_86418 Copy
Species: Thermatoga maritima
Genetic Insert: sfGFP with a 30aa CLP tag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5000; Vector Backbone:pET14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27224728
Proper citation: RRID:Addgene_86406 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19578373
Proper citation: RRID:Addgene_86470 Copy
Species: Mus musculus
Genetic Insert: Notch1 (C-term)
Vector Backbone Description: Vector Backbone:pT3-EF1aH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22797301
Proper citation: RRID:Addgene_86500 Copy
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