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Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126184 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126185 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126188 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126201 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126200 Copy
Species: Synthetic
Genetic Insert: NLS-nCas9-NLS-P2A-EGFP
Vector Backbone Description: Backbone Marker:pCMV-BE1 (Addgene #73019); Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30995674
Proper citation: RRID:Addgene_123616 Copy
Species: Synthetic
Genetic Insert: nCas9-UGI-NLS-P2A-EGFP
Vector Backbone Description: Backbone Marker:pSQT817 (Addgene #53373); Backbone Size:4843; Vector Backbone:CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30995674
Proper citation: RRID:Addgene_123611 Copy
Species: Synthetic
Genetic Insert: ArchT-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4763; Vector Backbone:AAV-Syn-FLEX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21811444
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_123607 Copy
Species: Synthetic
Genetic Insert: FLARE16-S aadA-GFP
Vector Backbone Description: Vector Backbone:pMK-T; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. This insert is a coding sequence designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123562 Copy
Species: Synthetic
Genetic Insert: crRNA scaffold 4n96
Vector Backbone Description: Vector Backbone:pUC19, BsaI site mutated; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123640 Copy
Species: Synthetic
Genetic Insert: AsCas12a
Vector Backbone Description: Vector Backbone:pCAG-2AeGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123638 Copy
Species: Synthetic
Genetic Insert: BsCas12a
Vector Backbone Description: Vector Backbone:pCAG-2AeGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123633 Copy
Species: Synthetic
Genetic Insert: LpCas12a
Vector Backbone Description: Vector Backbone:pCAG-2AeGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123635 Copy
Species: Synthetic
Genetic Insert: AnnexinA5
Vector Backbone Description: Vector Backbone:pDEST-Tol2-pA2 a-cry-mCherry; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_123628 Copy
Species: Synthetic
Genetic Insert: Connector L6-F1 insulator
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 1 parts are 5' assembly connectors in the MTK. Insulator sequences from https://doi.org/10.1038/nbt.3062 Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123690 Copy
Species: Synthetic
Genetic Insert: Connector L3-P2A
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 1 parts are 5' assembly connectors in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123694 Copy
Species: Synthetic
Genetic Insert: Connector L2-P2A
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 1 parts are 5' assembly connectors in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123693 Copy
Species: Synthetic
Genetic Insert: pUAS (mini SV40)
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 2 parts are promoters in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123698 Copy
Species: Synthetic
Genetic Insert: TetOn3G
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 3 parts are generally reserved for coding sequences in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123725 Copy
Species: Synthetic
Genetic Insert: Connector L8
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 1 parts are 5' assembly connectors in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123681 Copy
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