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Species: Mus musculus
Genetic Insert: GABA(A)R, Alpha4 (Rattus norvegicus) recombinant scFV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2922747.
Proper citation: RRID:Addgene_190551 Copy
Species: Caenorhabditis elegans
Genetic Insert: gpa-3
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_191089 Copy
Species: Mus musculus
Genetic Insert: Rufy3 (Mus musculus) recombinant scFV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2922752.
Proper citation: RRID:Addgene_190557 Copy
Species: Mus musculus
Genetic Insert: GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2922750.
Proper citation: RRID:Addgene_190555 Copy
Species: Mus musculus
Genetic Insert: Rufy3 (Mus musculus) recombinant scFV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2922754.
Proper citation: RRID:Addgene_190559 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184956 Copy
Species: Caenorhabditis elegans
Genetic Insert: gpa-13
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_191087 Copy
Species: Caenorhabditis elegans
Genetic Insert: gpa-11
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_191086 Copy
Species: Caenorhabditis elegans
Genetic Insert: glr-8
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_191085 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MFA-IgKappa-FAPoptim-STE3
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The pRS415-TEF1pr-MFA-NFAPoptim plasmid was used to insert the Ste3 coding sequence as an in-frame N-terminal fusion to FAP. The Ste3 coding sequence (lacking the stop codon) was inserted into the SmaI and XhoI restriction enzyme sites. The resulting plasmid contains the MFA1 ER targeting and secretion sequence, an N-terminal FAPoptim tag on Ste3.
Proper citation: RRID:Addgene_221114 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MFA-IgKappa-FAPoptim-STE3-K424R
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The lysine at position 242 in Ste3s C-tail was mutated to arginine using site-directed mutagenesis of pRS415-STE3pr-MFA-Igkappa-FAP-STE3.
Proper citation: RRID:Addgene_221119 Copy
Species: Synthetic
Genetic Insert: SaSp D10A Cas9
Vector Backbone Description: Backbone Marker:Genscript; Vector Backbone:pUC57 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2023.10.24.563270 for bioRxiv preprint.
Proper citation: RRID:Addgene_210734 Copy
Species: Mus musculus
Genetic Insert: Estrogen Receptor linked KLF2 T2A mCherry
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39946463
Proper citation: RRID:Addgene_224297 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39946463
Proper citation: RRID:Addgene_224298 Copy
Species: Homo sapiens
Genetic Insert: U6 sgRNA cassette with CD2 under PGK promoter
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39946463
Proper citation: RRID:Addgene_224294 Copy
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The Linker-MYC-FAPoptim-MYC sequence was cloned into the SalI and XhoI sites of the pRS413-MET25pr plasmid.
Proper citation: RRID:Addgene_221105 Copy
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The TEF1 promoter from the pRS413-TEF1pr plasmid (Mumberg et al., 1995) was removed by restriction digestion and replaced with the MET25 promoter (PCR amplified from the pUG vector series) flanked by SacI and XbaI restriction sites.
Proper citation: RRID:Addgene_221103 Copy
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The Linker-MYC-FAPoptim-MYC sequence was cloned into the SalI and XhoI sites of the pRS415-MET25pr plasmid.
Proper citation: RRID:Addgene_221102 Copy
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The TEF1 promoter from the pRS413-TEF1pr plasmid (Mumberg et al., 1995) was removed by restriction digestion and replaced with the CUP1 promoter (PCR amplified from the pKK212 plasmid used in (O'Donnell et al., 2013) flanked by SacI and BamHI restriction sites.
Proper citation: RRID:Addgene_221109 Copy
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The Linker-MYC-FAPoptim-MYC sequence was cloned into the SalI and XhoI sites of the pRS415-CUP1pr plasmid.
Proper citation: RRID:Addgene_221108 Copy
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