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Species: Mus musculus
Genetic Insert: CA-RIT-NFAT1
Vector Backbone Description: Backbone Marker:Anjana Rao lab; Vector Backbone:pRV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25680272
Comments: RIT mutations (R468, I469, and T535) selectively interfere with NFAT:AP-1 interaction
Proper citation: RRID:Addgene_85181 Copy
Species: Homo sapiens
Genetic Insert: Hsp27
Vector Backbone Description: Vector Backbone:pFLAG-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The pFLAG-CMV2-Hsp27 wild-type plasmid was obtained from Dr. Park and is described by Park KJ, Gaynor RB, Kwak YT. J Biol Chem 2003; 278:35272-35278. PMID:12829720
Proper citation: RRID:Addgene_85188 Copy
Species: Homo sapiens
Genetic Insert: human mature (delta25) MCAD
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25416781
Comments: Homo sapiens wt MCAD, delta 25, cloned into NdeI-NotI sites of pET28a, contains a N-terminal His-tag
Proper citation: RRID:Addgene_85112 Copy
Species: Homo sapiens
Genetic Insert: human mature (delta44) GCDH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25416781
Comments: Homo sapiens wt GCDH, delta 44, cloned into NdeI-NotI sites of pET28a, contains a N-terminal His-tag
Proper citation: RRID:Addgene_85113 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pSicoR_mCherry without PuroR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798611
Proper citation: RRID:Addgene_85412 Copy
Genetic Insert: puromycin resistance
Vector Backbone Description: Vector Backbone:Addgene #61427; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798611
Proper citation: RRID:Addgene_85413 Copy
Genetic Insert: dCas9 and Blasticidin resistance
Vector Backbone Description: Vector Backbone:Addgene #61425; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798611
Proper citation: RRID:Addgene_85417 Copy
Species: Mus musculus
Genetic Insert: HOXB9
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8657149
Proper citation: RRID:Addgene_8541 Copy
Species: Homo sapiens
Genetic Insert: HOXB7
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:2573064
Comments: Simeone et al. Proc. Natl. Acad. Sci. USA 84: 4914-18, 1987
Proper citation: RRID:Addgene_8538 Copy
Species: Homo sapiens
Genetic Insert: HOXB7
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3005; Vector Backbone:pSP65; Vector Types:Bacterial Expression, In vitro transcription/translation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2573064
Comments: can be used for in vitro TNT
Proper citation: RRID:Addgene_8537 Copy
Species: Homo sapiens
Genetic Insert: HOXB5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2950; Vector Backbone:Bluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2573064
Proper citation: RRID:Addgene_8531 Copy
Species: GU461306
Genetic Insert: humanized photoactivated adenylyl cyclase
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Insert was described in Stierl et al Biochemistry. 2014 Aug 12;53(31):5121-30. doi: 10.1021/bi500479v. Epub 2014 Jul 28 and cloned behind a neuron-specific promoter.
Proper citation: RRID:Addgene_85398 Copy
Species: Chlamydomonas
Genetic Insert: Channelrhodopsin-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5802; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21504945
Comments: Layer-specific optogenetic activation of pyramidal neurons causes beta-gamma entrainment of neonatal networks.
Nat Commun. 2017 Feb 20;8:14563. doi: 10.1038/ncomms14563.
Bitzenhofer SH1, Ahlbeck J1, Wolff A1, Wiegert JS2, Gee CE2, Oertner TG2, Hanganu-Opatz IL1.
Proper citation: RRID:Addgene_85399 Copy
Species: Synthetic
Genetic Insert: Optimized G Protein
Vector Backbone Description: Vector Backbone:pAAV-syn-FLEX-splitTVA-eGFP-B19G IW; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_85225 Copy
Species: Homo sapiens
Genetic Insert: GBP1 (guanylate binding protein 1)
Vector Backbone Description: Backbone Size:8919; Vector Backbone:pLKO.1-TRC.mKO2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29115931
Comments: Also, see Addgene's pLKO.1 protocol http://www.addgene.org/plko on how to use the pLKO.1 vector.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_85210 Copy
Species: Synthetic
Genetic Insert: EBFP2
Vector Backbone Description: Backbone Size:6417; Vector Backbone:LeGO vector; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28109958
Comments: HIV-1 derived third generation lentiviral vector for stable cell marking. Use second generation (like psPAX2) or third generation (like pMDLg/pRRE + pRSV-Rev) systems for packaging in addition to a VSV-G expressing plasmid (or another envelope protein). Please visit the LeGO-Vector home page for more information: http://www.LentiGO-Vectors.de
The BsrGI site of EBFP2 can be used to fuse an antibiotic resistance to the fluorescent protein following the building block principle of the LeGO vector system. More information is available on the LeGO website and in the following publications.
More references:
Weber et al., Molecular Therapy, 2008:
http://www.nature.com/mt/journal/v16/n4/full/mt20086a.html
Weber et al., Gene Therapy, 2010:
http://www.nature.com/gt/journal/v17/n4/full/gt2009149a.html
Proper citation: RRID:Addgene_85213 Copy
Vector Backbone Description: Backbone Size:10378; Vector Backbone:pLentiGuide; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27458201
Proper citation: RRID:Addgene_85401 Copy
Genetic Insert: MS2-AID*Δ and Hygromycin resistance
Vector Backbone Description: Vector Backbone:Addgene #61426; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798611
Proper citation: RRID:Addgene_85406 Copy
Species: Other
Genetic Insert: Flag-iCas9-P2A-GFP
Vector Backbone Description: Backbone Size:10000; Vector Backbone:pInducer-20; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27458201
Proper citation: RRID:Addgene_85400 Copy
Genetic Insert: anti-Her2_4D5-8_synNotch
Vector Backbone Description: Vector Backbone:pHR SIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27693353
Proper citation: RRID:Addgene_85425 Copy
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