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Species: Homo sapiens
Genetic Insert: APOBEC3C (A3C)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28315663
Proper citation: RRID:Addgene_105051 Copy
Species: Homo sapiens
Genetic Insert: eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29183983
Proper citation: RRID:Addgene_105244 Copy
Species: Homo sapiens
Genetic Insert: eGFP with homology arms for Lamin A/C
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29183983
Proper citation: RRID:Addgene_105245 Copy
Species: Homo sapiens
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29183983
Proper citation: RRID:Addgene_105242 Copy
Species: Homo sapiens
Genetic Insert: GFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29183983
Proper citation: RRID:Addgene_105243 Copy
Species: Homo sapiens
Genetic Insert: sgRNA for PYM1
Vector Backbone Description: Vector Backbone:pX458; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29183983
Proper citation: RRID:Addgene_105249 Copy
Species: Homo sapiens
Genetic Insert: gRNA_hIRF1 promoter #351
Vector Backbone Description: Backbone Marker:Keith Joung (Addgene plasmid #70709); Backbone Size:2288; Vector Backbone:BPK2660; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29482606
Proper citation: RRID:Addgene_105283 Copy
Species: Homo sapiens
Genetic Insert: Kindlin2
Vector Backbone Description: Backbone Marker:modified pEGFP-N1; Vector Backbone:pEGFP-NV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25646088
Comments: Mutations in Kindlin2 were introduced by using site-directed mutagenesis (Stratagene)
Proper citation: RRID:Addgene_105309 Copy
Species: Homo sapiens
Genetic Insert: Kindlin2
Vector Backbone Description: Backbone Marker:modified pEGFP-N1; Vector Backbone:pEGFP-NV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25646088
Comments: Mutation in Kindlin2 was introduced by using site-directed mutagenesis (Stratagene)
Proper citation: RRID:Addgene_105308 Copy
Species: Homo sapiens
Genetic Insert: Kindlin2
Vector Backbone Description: Backbone Marker:modified pEGFP-N1; Vector Backbone:pEGFP-NV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25646088
Comments: Mutation in Kindlin2 was introduced by using site-directed mutagenesis (Stratagene)
Proper citation: RRID:Addgene_105306 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting human MYB DBD
Vector Backbone Description: Vector Backbone:LRG 2.1T(hU6-sgRNA- EFS-GFP-P2A-Neo); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Comments: Addgene's sequencing found I2L and S177R in NeoR. These mutations are not known to affect neomycin selection.
Proper citation: RRID:Addgene_105592 Copy
Species: Homo sapiens
Genetic Insert: Human IgG1 Fc-domain fused with GFP nanobody
Vector Backbone Description: Backbone Size:3100; Vector Backbone:pGEMHE; Vector Types:in vitro Transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29153837
Proper citation: RRID:Addgene_105576 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting human MYB DBD
Vector Backbone Description: Vector Backbone:LRG 2.1T(hU6-sgRNA- EFS-GFP-P2A-Neo); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Comments: Addgene's sequencing found I2L and S177R in NeoR. These mutations are not known to affect neomycin selection.
Proper citation: RRID:Addgene_105591 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting MLL part of MLL-AF9 fusion
Vector Backbone Description: Vector Backbone:LEP (MSCV-LTR-mirE-shRNA-PGK-puro-IRES-GFP); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Proper citation: RRID:Addgene_105565 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting MLL part of MLL-AF9 fusion
Vector Backbone Description: Vector Backbone:LEP (MSCV-LTR-mirE-shRNA-PGK-puro-IRES-GFP); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316427
Proper citation: RRID:Addgene_105566 Copy
Species: Homo sapiens
Genetic Insert: mCerulean-P2A-putative cMYC DBD with a HA tag
Vector Backbone Description: Backbone Marker:Michel Sadelain (Addgene plasmid # 23244); Vector Backbone:pLM-mCerulean-cMYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28813671
Proper citation: RRID:Addgene_100125 Copy
Species: Homo sapiens
Genetic Insert: miR-183-96-82 Cluster promoter 0.8 Kb Fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4322; Vector Backbone:pGL4; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28412746
Comments: Addgene's sequencing results found two discrepancies compared to the depositor's sequence: 123_124insAATT, 162delG, & 854G>T. Some differences could be SNPs and do not occur near the binding sites of the transcription factors of interest; thus they should not affect plasmid function.
Proper citation: RRID:Addgene_100129 Copy
Species: Homo sapiens
Genetic Insert: miR-183-96-82 Cluster promoter 4.8Kb Fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4334; Vector Backbone:pGL4; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28412746
Comments: Addgene's sequencing results found the following discrepancies compared to the depositor's sequence: 367A>G, 739G>A, 928_929insA, 945delA, 1009C>T, 1682G>A, 2324A>T, 2503G>C, 3233A>G, 4092delG, 4784G>T. These differences could be SNPs and do not occur near the binding sites of the transcription factors of interest; thus they should not affect plasmid function.
Proper citation: RRID:Addgene_100127 Copy
Species: Homo sapiens
Genetic Insert: STAR protein
Vector Backbone Description: Backbone Size:4000; Vector Backbone:HMBP-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26555181
Comments: The STARD1 domain sequence was subcloned after 3C site to obtain 3C cleavable His6-MBP-3C-STAR construction.
Proper citation: RRID:Addgene_100094 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma human
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28-his-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28931924
Comments: The 3C site was introduced immediately prior to 14-3-3 sequence to facilitate purification by subtractive IMAC.
Proper citation: RRID:Addgene_100093 Copy
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