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Species: Drosophila melanogaster
Genetic Insert: CG17293
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_71079 Copy
Species: Drosophila melanogaster
Genetic Insert: 770_sn-EcR_dCP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_71221 Copy
Species: Drosophila melanogaster
Genetic Insert: 769_DipB-EcR_dCP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_71222 Copy
Species: Drosophila melanogaster
Genetic Insert: 768_Nhe2-EcR_dCP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_71223 Copy
Species: Drosophila melanogaster
Genetic Insert: Copia
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4526; Vector Backbone:pCoHygro; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_71389 Copy
Species: Drosophila melanogaster
Genetic Insert: RpL10Ab
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22792260
Comments: The 5 ' restriction site used for cloning is NotI (EcoRI, BglII are 5' to that site) and there is an internal XhoI site at bp 84 of the 654 bp RpL10Ab fragment. There is a unique KpnI site immediately following the XhoI site at the 3' end of the cloned fragment. The NotI site and BglII sites are also usable for anyone wanting to reclone the fragment and the XhoI site is not usable but the KpnI site is.
Proper citation: RRID:Addgene_71729 Copy
Species: Drosophila melanogaster
Genetic Insert: Dscam1_7.27.22
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: This transcript contains altrenative exons 4.7, 6.27 and 9.22.
Proper citation: RRID:Addgene_72060 Copy
Species: Drosophila melanogaster
Genetic Insert: Dscam1_7.27.26
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: This transcript contains altrenative exons 4.7, 6.27 and 9.26.
Proper citation: RRID:Addgene_72063 Copy
Species: Drosophila melanogaster
Genetic Insert: Dscam1_7.27.30
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: This transcript contains altrenative exons 4.7, 6.27 and 9.30.
Proper citation: RRID:Addgene_72064 Copy
Species: Drosophila melanogaster
Genetic Insert: Dscam1_7.27.26
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: This transcript contains altrenative exons 4.7, 6.27 and 9.26.
Proper citation: RRID:Addgene_72189 Copy
Species: Drosophila melanogaster
Genetic Insert: Dscam1_7.27.22
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: This transcript contains altrenative exons 4.7, 6.27 and 9.22.
Proper citation: RRID:Addgene_72186 Copy
Species: Drosophila melanogaster
Genetic Insert: dmBACCS2VL-IRES-dOrai-IRES-GFP
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4581; Vector Backbone:pWPRE/C1 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26282514
Comments: dmBACCS2VL is a slow recovery kinetic mutant of dmBACCS2.
Proper citation: RRID:Addgene_72897 Copy
Species: Drosophila melanogaster
Genetic Insert: dmBACCS2-IRES-dOrai-IRES-mCherry
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4581; Vector Backbone:pWPRE/C1 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26282514
Proper citation: RRID:Addgene_72894 Copy
Species: Drosophila melanogaster
Genetic Insert: dmBACCS2NS-IRES-dOrai-IRES-GFP
Vector Backbone Description: Backbone Marker:Modified from Clontech; Backbone Size:4581; Vector Backbone:pWPRE/C1 (modified pEGFP-C1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26282514
Comments: dmBACCS2NS is a fast recovery kinetic mutant of dmBACCS2.
Proper citation: RRID:Addgene_72895 Copy
Species: Drosophila melanogaster
Genetic Insert: crumbs
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Comments: The plasmid contains part of the 5' cDNA of Crb and the 3' part that brings the transmembrane and the intracellular domains of the protein together. The BglII restriction site links the 5' and 3' regions of the cDNA, meaning that the majority of the extracellular part of the Crb protein is missing. The fragment also contains the 5' and 3' UTR regions. BglII restriction site was used to clone a GFP fragment. GFP is inserted between aa 96 and 2122 of crb. Crb contains K89E mutation which does not affect plasmid function.
Proper citation: RRID:Addgene_67525 Copy
Species: Drosophila melanogaster
Genetic Insert: dyl - chitin binding domain - tdTomato - dyl
Vector Backbone Description: Backbone Marker:Norbert Perrimon; Backbone Size:7541; Vector Backbone:pWalium-moe; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26395478
Comments: The plasmid contains pieces of DNA from a number of different sources. The td-Tomato was obtained from FUtdTW, Addgene.
Chitin binding domain was purchased from New England Biolabs
The dyl cDNA came from the Drosophila genome project
The pWalium10 moe vector came originally from the N. Perrimon Lab.
Proper citation: RRID:Addgene_67756 Copy
Species: Drosophila melanogaster
Genetic Insert: LN-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:LamdaN entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128012 Copy
Species: Drosophila melanogaster
Genetic Insert: Gal4-DBD-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:Gal4 entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128014 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila melanogaster Shaker cognate l
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pBSc; Vector Types:Xenopus Oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2333511
Proper citation: RRID:Addgene_16196 Copy
Species: Drosophila melanogaster
Genetic Insert: UAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
Vector Backbone Description: Backbone Marker:Pfeiffer et al; Backbone Size:7831; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21297621
Comments: This plasmid contains mTFP1.0, and not EBFP2, as suggested by the linear plasmid map image file.
Please note that Addgene's sequencing results identified a few discrepancies when compared to GenBank ID JF267350. The depositing laboratory states that these differences are not a concern for the function of the plasmid. The full plasmid sequence has been assembled and not entirely confirmed by sequencing. Additional discrepancies may exist.
This plasmid was constructed using the following restriction enyzmes:
Bgl II / Not I - loxP sites
Not I / Xho I - EGFP-HSV
Xho I / Kpn I - mTFP1.0-HA
Kpn I / Xba I - mKO2-V5
NotI is not a unique cutter--it is present twice in the plasmid. The XbaI site has been confirmed by Addgene's sequencing results, but it not present in the full plasmid sequence.
Proper citation: RRID:Addgene_29327 Copy
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