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Species: Zea mays
Genetic Insert: MYBGA transcription factor from DR808352
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27833 Copy
Species: Zea mays
Genetic Insert: FHA transcription factor from EC886186
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27830 Copy
Genetic Insert: mAmber N1
Vector Backbone Description: Backbone Marker:Addgene plasmid# 27793; Backbone Size:3984; Vector Backbone:mVenus N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17040988
Comments: Amber was created by mutating Tyrosine 67 in Venus to Cysteine (Y67C).
See Addgene's sequencing results for detailed MCS and Amber. This plasmid is designed to clone a gene of interest upstream and in-frame to create a C-terminal Amber fusion protein.
Proper citation: RRID:Addgene_27798 Copy
Genetic Insert: mCerulean N1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3984; Vector Backbone:pECFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17040988
Comments: This construct was created by mutagenesis of pECFP-N1. When compared with ECFP, Cerulean contains the following mutations: S72A, Y145A and H148D. This construct also contains A206K mutation to create a monomeric form of the fluorescent protein.
See Addgene's sequencing results for detailed MCS and mCerulean. This plasmid is designed to clone a gene of interest upstream and in-frame to create a C-terminal mCerulean fusion protein.
Proper citation: RRID:Addgene_27795 Copy
Genetic Insert: mCerulean C1
Vector Backbone Description: Backbone Size:3984; Vector Backbone:pECFP C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17040988
Comments: This construct was created by mutagenesis of pECFP-C1. When compared with ECFP, Cerulean contains the following mutations: S72A, Y145A and H148D. This construct also contains A206K mutation to create a monomeric form of the fluorescent protein.
See Addgene's sequencing results for detailed mCerulean and MCS. This plasmid is designed to clone a gene of interest downstream and in-frame to create a N-terminal mCerulean fusion protein.
Proper citation: RRID:Addgene_27796 Copy
Genetic Insert: Amber-Cerulean-Venus
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3940; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Stoichiometry standard; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19946626
Comments: Amber can be excised using a NheI-BSP E1 double digest; Cerulean by BglII-SalI and Venus by SalI-BamHI. The full insert can be released by a NheI-BamHI double-digest.
Due to the multiple fluorescent protein repeats in this plasmid, Addgene is unable to confirm the entire insert by sequencing. Please see Addgene's diagnostic digest for this plasmid by clicking the Notes from Addgene link above.
Proper citation: RRID:Addgene_27791 Copy
Species: Zea mays
Genetic Insert: VOZ transcription factor from DV516566
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27847 Copy
Species: Zea mays
Genetic Insert: MYB transcription factor from EB402539
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27845 Copy
Species: Zea mays
Genetic Insert: TCP transcription factor from DR792871
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27846 Copy
Species: Zea mays
Genetic Insert: AP2-EREBP transcription factor from DV529833
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27843 Copy
Species: Zea mays
Genetic Insert: NAC transcription factor from DR787066
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27844 Copy
Species: Zea mays
Genetic Insert: ARF transcription factor from EE033875
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27841 Copy
Species: Oryza sativa (rice)
Genetic Insert: WRKY transcription factor encoded by Os01g0626400 LOC_Os01g43650
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27939 Copy
Species: Oryza sativa (rice)
Genetic Insert: bHLH transcription factor encoded by Os02g0116600 LOC_Os02g02480
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27936 Copy
Species: Oryza sativa (rice)
Genetic Insert: WRKY transcription factor encoded by Os11g0490900 LOC_Os11g29870
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27933 Copy
Species: Oryza sativa (rice)
Genetic Insert: bHLH transcription factor encoded by Os03g0135700 LOC_Os03g04310
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27931 Copy
Species: Oryza sativa (rice)
Genetic Insert: bHLH transcription factor encoded by Os02g0433600 LOC_Os02g23823
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/SD entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27932 Copy
Species: Oryza sativa (rice)
Genetic Insert: MYB transcription factor encoded by Os05g0140100 LOC_Os05g04820
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/D entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27897 Copy
Species: Oryza sativa (rice)
Genetic Insert: MYB-related transcription factor encoded by Os05g0589400 LOC_Os05g51160
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/D entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27893 Copy
Species: Oryza sativa (rice)
Genetic Insert: CCAAT transcription factor encoded by Os07g0545400 LOC_Os07g36140
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987217
Comments: This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/D entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27890 Copy
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