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Species: Caenorhabditis elegans
Genetic Insert: [BAGp | gfp | tbb-2 UTR]
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:Twist Amp High; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See www.wormbuilder.org/BioParts for more information.
Proper citation: RRID:Addgene_200340 Copy
Species: Caenorhabditis elegans
Genetic Insert: 15xUAS::TeTX-SL2-mKate::let-858 3'UTR
Vector Backbone Description: Backbone Marker:Fire Lab C. elegans Vector Kit; Vector Backbone:pPD117.01; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096407
Proper citation: RRID:Addgene_198835 Copy
Species: Caenorhabditis elegans
Genetic Insert: wmCardinal-T2A-HO1
Vector Backbone Description: Vector Backbone:pSF11; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37666982
Comments: Please visit https://www.researchsquare.com/article/rs-797586/v1 for preprint.
Proper citation: RRID:Addgene_197249 Copy
Species: Caenorhabditis elegans
Genetic Insert: wmiRFP720-T2A-HO1
Vector Backbone Description: Vector Backbone:pSF11; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37666982
Comments: Please visit https://www.researchsquare.com/article/rs-797586/v1 for preprint.
Proper citation: RRID:Addgene_197248 Copy
Species: Caenorhabditis elegans
Genetic Insert: wmNeonGreen
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37666982
Comments: Please visit https://www.researchsquare.com/article/rs-797586/v1 for preprint.
Proper citation: RRID:Addgene_197242 Copy
Species: Caenorhabditis elegans
Genetic Insert: cec-4 (C. elegans)
Vector Backbone Description: Backbone Size:6442; Vector Backbone:pHIT198 (derived from pET-28a); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37078421
Proper citation: RRID:Addgene_204518 Copy
Species: Caenorhabditis elegans
Genetic Insert: C-terminal fragment of coh-3 (C. elegans)
Vector Backbone Description: Backbone Size:6442; Vector Backbone:pHIT198 (derived from pET-28a); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37078421
Proper citation: RRID:Addgene_204516 Copy
Species: Caenorhabditis elegans
Genetic Insert: Internal fragment of him-1 (C. elegans)
Vector Backbone Description: Backbone Size:6442; Vector Backbone:pHIT198 (derived from pET-28a); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37078421
Proper citation: RRID:Addgene_204517 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mos1_transposase::glh-2_3'UTR
Vector Backbone Description: Backbone Size:3073; Vector Backbone:pHIT88 (derived from pCITE-4b); Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37078421
Comments: T7p, SL1, StuI, Mos1 transposase, NheI, glh-2 3’UTR, XbaI, a 61-nt poly(A), BsaI and SpeI; linearize with BsaI and use for in vitro transcription of mRNA
Proper citation: RRID:Addgene_204522 Copy
Species: Caenorhabditis elegans
Genetic Insert: avr-15 sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24879462
Comments: gRNA target sequence GTTTGCAATATAAGTCACCC
Proper citation: RRID:Addgene_58982 Copy
Species: Caenorhabditis elegans
Genetic Insert: avr-14 sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24879462
Comments: gRNA target sequence GATTGGAGAGTTAGACCACG
Proper citation: RRID:Addgene_58981 Copy
Species: Caenorhabditis elegans
Genetic Insert: Blasticidin S resistance
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:1961; Vector Backbone:pBluescript KS(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24879462
Proper citation: RRID:Addgene_59181 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::GFP
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Comments: The Pmyo-2::GFP cassette is inserted in place of a fluorescent protein in the assembled repair template. This is intended for knocking out a gene and replacing it with Pmyo-2::GFP.
Proper citation: RRID:Addgene_91828 Copy
Species: Caenorhabditis elegans
Genetic Insert: CTL2 (M222R) of PIEZO1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25242456
Comments: Note: M2225R mutation refers to human mutation of gene. M2092R is mutation in C. elegans.
Proper citation: RRID:Addgene_96952 Copy
Species: Caenorhabditis elegans
Genetic Insert: 1-209
Vector Backbone Description: Vector Backbone:pET28; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20462958
Proper citation: RRID:Addgene_59555 Copy
Species: Caenorhabditis elegans
Genetic Insert: 1-214
Vector Backbone Description: Vector Backbone:pET28; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20462958
Proper citation: RRID:Addgene_59550 Copy
Species: Caenorhabditis elegans
Genetic Insert: 1-211
Vector Backbone Description: Vector Backbone:pET28; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20462958
Proper citation: RRID:Addgene_59553 Copy
Species: Caenorhabditis elegans
Genetic Insert: cdc-42 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59786 Copy
Species: Caenorhabditis elegans
Genetic Insert: elt-2 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59784 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-109(n499) gRNA
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_59932 Copy
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