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Species: Homo sapiens
Genetic Insert: Receptor-interacting serine/threonine-protein kinase 3
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37072399
Proper citation: RRID:Addgene_199328 Copy
Species: Synthetic
Genetic Insert: BCD 25K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200045 Copy
Species: Homo sapiens
Genetic Insert: FUSN
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37000871
Proper citation: RRID:Addgene_200967 Copy
Species: Homo sapiens
Genetic Insert: ACSL4 promoter (partial)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36928314
Proper citation: RRID:Addgene_202511 Copy
Species: Caenorhabditis elegans
Genetic Insert: [ASELp | gfp | tbb-2 UTR]
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:Twist Amp High; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See www.wormbuilder.org/BioParts for more information.
Proper citation: RRID:Addgene_200334 Copy
Species: Synthetic
Genetic Insert: BCD 0.75K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200054 Copy
Species: Synthetic
Genetic Insert: H5_fold-0_Elsa
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38177681
Proper citation: RRID:Addgene_201825 Copy
Species: Bacteriophage P1 (E. coli P1 lysogen EMG16)
Genetic Insert: part kilA/repL
Vector Backbone Description: Backbone Size:3197; Vector Backbone:pSC101 (4A3); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36846746
Proper citation: RRID:Addgene_197872 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: XI1_DW
Vector Backbone Description: Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37058502
Comments: Cloning Method: Goldon Gate Assembly
Proper citation: RRID:Addgene_198684 Copy
Species: Streptococcus pyogenes
Genetic Insert: trancCRNA
Vector Backbone Description: Backbone Marker:Christopher Anderson; Backbone Size:9334; Vector Backbone:Bba_J72114; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36802585
Proper citation: RRID:Addgene_197871 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: XI1_UP
Vector Backbone Description: Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37058502
Comments: Cloning Method: Goldon Gate Assembly
Proper citation: RRID:Addgene_198683 Copy
Species: Homo sapiens
Genetic Insert: E2F3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36928314
Proper citation: RRID:Addgene_202522 Copy
Species: Caenorhabditis elegans
Genetic Insert: [OLQp | wrmScarlet | tbb-2 UTR]
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:Twist Amp High; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See www.wormbuilder.org/BioParts for more information.
Proper citation: RRID:Addgene_200343 Copy
Species: Homo sapiens
Genetic Insert: E2F1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36928314
Proper citation: RRID:Addgene_202520 Copy
Species: Caenorhabditis elegans
Genetic Insert: [PVPp | wrmScarlet | tbb-2 UTR]
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:Twist Amp High; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See www.wormbuilder.org/BioParts for more information.
Proper citation: RRID:Addgene_200341 Copy
Species: Burkholderia cenocepacia
Genetic Insert: COX8A MTS-LacZ-G1397 DddAtox-N-UGI-ATP5B 3'UTR
Vector Backbone Description: Vector Backbone:pKmCAGS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34847747
Proper citation: RRID:Addgene_201397 Copy
Species: Caenorhabditis elegans
Genetic Insert: [PVPp | gfp | tbb-2 UTR]
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:Twist Amp High; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See www.wormbuilder.org/BioParts for more information.
Proper citation: RRID:Addgene_200342 Copy
Genetic Insert: HA-tagged CASP sigma
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36423276
Proper citation: RRID:Addgene_197614 Copy
Species: Synthetic
Genetic Insert: sgRNA1_1xCTS
Vector Backbone Description: Vector Backbone:p035_pause-HBG-CFP-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200237 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens centromere protein B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31744372
Proper citation: RRID:Addgene_167569 Copy
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