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Species: Homo sapiens
Genetic Insert: NOLC1 nicking sgRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor (Addgene #132777); Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39676077
Proper citation: RRID:Addgene_219861 Copy
Species: Homo sapiens
Genetic Insert: NOLC1 atgRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor (Addgene #132777); Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39676077
Proper citation: RRID:Addgene_219860 Copy
Species: Pichia Pastoris
Genetic Insert: ZWF1
Vector Backbone Description: Backbone Marker:Michael Sauer (Sarkari et al. 2017) Addgene #89917; Vector Backbone:BB2_L_AB_syn_BbsI; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_222593 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA1_LCD_5W D262V
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38464104
Comments: Insert created through codon optimized gene synthesis. TEV cleavage leaves N-terminal GS tag.
Please visit https://doi.org/10.1101/2024.02.28.582569 for bioRxiv preprint.
Proper citation: RRID:Addgene_234617 Copy
Species: Escherichia phage T4
Genetic Insert: 46
Vector Backbone Description: Backbone Marker:New England BioLabs; Vector Backbone:pTBY1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21081488
Comments: Please note: This plasmid contains a T144I mutation in T4gp46. This mutation is not known to impact plasmid function.
Proper citation: RRID:Addgene_228211 Copy
Vector Backbone Description: Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V3.0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39939860
Comments: Contains TadA, TadA* (7.10), Cas9(D10A) . Please visit https://doi.org/10.1101/2024.07.19.604224 for bioRxiv preprint.
Proper citation: RRID:Addgene_226956 Copy
Species: Homo sapiens
Genetic Insert: TUBG2
Vector Backbone Description: Backbone Marker:thermofisher; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40019206
Comments: The internal EcoRI was removed from the GeneCopeia mCherry tagged TUBG2/pReceiver-M56 plasmid and TUBG2 was sub-cloned into pcDNA3.1, and a Flag was added to the N-terminal region of TUBG2.
To create a TUBG2 gene resistant to human pTER gamma-tubulin shRNA (Addgene plasmid #87955) and single guide RNA TUBG2, the following silent mutations have been introduced:
G to A at position 30
C to A at position 33
C to T at position 1236
C to T at position 1242
Proper citation: RRID:Addgene_229855 Copy
Species: Synthetic
Genetic Insert: Octo_VirD2_NLS-Cas9-6xHis
Vector Backbone Description: Backbone Marker:David Liu; Backbone Size:5140; Vector Backbone:pET-NLS-Cas9-6xHis (Addgene Plasmid #62934); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40030016
Proper citation: RRID:Addgene_232296 Copy
Genetic Insert: rab35
Vector Backbone Description: Vector Backbone:p1074; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39804967
Proper citation: RRID:Addgene_226764 Copy
Species: Synthetic
Genetic Insert: Rluc-Y39x-Fluc
Vector Backbone Description: Vector Backbone:psiCHECK2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35674491
Proper citation: RRID:Addgene_233615 Copy
Vector Backbone Description: Vector Backbone:backbone; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39804967
Proper citation: RRID:Addgene_226768 Copy
Vector Backbone Description: Vector Backbone:backbone; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39804967
Proper citation: RRID:Addgene_226767 Copy
Genetic Insert: rab35-mutant Q67L
Vector Backbone Description: Vector Backbone:p1074; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39804967
Proper citation: RRID:Addgene_226765 Copy
Species: Synthetic
Genetic Insert: iCre
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Comments: 5' Cloning Site: MluI (not destroyed), 3' Cloning Site: SgrAI (not destroyed). Please visit https://doi.org/10.1101/2024.06.10.597244 for bioRxiv preprint.
Proper citation: RRID:Addgene_220708 Copy
Species: Homo sapiens
Genetic Insert: ZDHHC22
Vector Backbone Description: Vector Backbone:pECMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39546589
Proper citation: RRID:Addgene_231670 Copy
Vector Backbone Description: Vector Backbone:pHCMV-Firefly-Luciferase; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39755699
Proper citation: RRID:Addgene_232920 Copy
Species: Other
Genetic Insert: Anti-amyloid-β 40/42 nanobody (R3VQ)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37961104
Comments: Please visit https://doi.org/10.1101/2023.10.24.563674 for bioRxiv preprint.
Proper citation: RRID:Addgene_233217 Copy
Species: Synthetic
Genetic Insert: gRNA targeting EGFP
Vector Backbone Description: Vector Backbone:pUC016; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38467613
Proper citation: RRID:Addgene_233613 Copy
Species: Homo sapiens
Genetic Insert: GAG FMLV fused to PEmax
Vector Backbone Description: Vector Backbone:pCDNA3-like; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39755699
Comments: Addgene observed 1 base pair mismatch in the ORF leading to H102Y substitution. This discrepancy was found to not affect plasmid function.
Proper citation: RRID:Addgene_232921 Copy
Species: Homo sapiens
Genetic Insert: ZDHHC14
Vector Backbone Description: Vector Backbone:pECMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39546589
Proper citation: RRID:Addgene_231662 Copy
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