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Species: Homo sapiens
Genetic Insert: ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control pegRNA vector derived from ATP1A1_G4_Q118R_Dual_pegRNA. This vector can be used as a positive control for prime editing (PE3 in combination with ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA) to co-target ATP1A1 and RUNX1 to perform coselection for RUNX1 +1 ATG insertion. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173213 Copy
Genetic Insert: mScarlet-mNeonGreen
Vector Backbone Description: Backbone Size:5614; Vector Backbone:pFA6a-mCherry-sfGFP-kanMX (pMaM17); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34985695
Proper citation: RRID:Addgene_173455 Copy
Species: Synthetic
Genetic Insert: gRNA-12xMBS
Vector Backbone Description: Backbone Size:7514; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173178 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing (PE3); Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control PE3 nick sgRNAs vector derived from ATP1A1_G3_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 and MTOR to perform coselection for MTOR hyperactivating mutation E2419K. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173211 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173451 Copy
Species: Synthetic
Genetic Insert: gRNA-12xMBS backbone
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4837; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173175 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173450 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Size:6479; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173173 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 C-FLAG; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17316 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17317 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 C-FLAG; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17320 Copy
Species: Mus musculus
Genetic Insert: MED10
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Comments: The Med10 sequence was inserted between XhoI and Kpn1 of pBacPAK8 polylinker after changing the original BacPAK8 BamHI site to NheI. Plasmid can be used for Med10 expression or, by cutting out and replacing Med10 CDS with something else, for construction of BacPAK8 derivatives expressing 2xHA-tagged proteins.
Proper citation: RRID:Addgene_17321 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17324 Copy
Species: Streptomyces avidinii
Genetic Insert: streptavidin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7499314
Proper citation: RRID:Addgene_17327 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G4 Q118R pegRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Prime editing vector derived from pU6-pegRNA-GG-acceptor. See Anzalone et al. Nature (2019) for the detailed pegRNA cloning protocol to target your gene of interest. False-positive colonies will appear pink or red if the RFP insert has not been replaced by the new pegRNA cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173199 Copy
Species: Streptomyces avidinii
Genetic Insert: streptavidin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7724536
Comments: pTSA-38 is a derivative of pTSA-13 with a W120F mutation.
In brief, mpSA-29 was generated by inserting
a 480-bp fragment of pTSA-13 carrying the entire coding
sequence into M13mpl8. Oligonucleotide-directed in vitro
mutagenesis was performed on mpSA-29 by using the
30-nt oligonucleotide, 5'-ACCAGCGTGGACTTGAAG-
GCGTTGGCCTCG-3', to convert the TGG codon for Trp-
120 to TTC for Phe (mpSA-36). Then, a 405-bp fragment of
mpSA-36 carrying the entire coding sequence was cloned into
pET-3a. The resulting expression vector, pTSA-38, encodes a streptavidin W120F mutant.
Proper citation: RRID:Addgene_17328 Copy
Species: Synthetic
Genetic Insert: Hyper7-tdTomato
Vector Backbone Description: Backbone Size:4268; Vector Backbone:pAAV-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35226474
Proper citation: RRID:Addgene_173198 Copy
Species: Streptomyces avidinii
Genetic Insert: streptavidin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11716692
Comments: A PCR-based site-directed mutagenesis method was used to modify the coding sequence of pTSA-13. A DNA sequence encoding six amino acid residues with a translation termination codon, GGT GGT TCT GGT TGC CCG TAG (Gly-Gly-Ser-Gly-Cys-Pro-Stop), was fused to the codon for Val-133 (GTG). The entire coding sequence was cloned between the NdeI and BamHI sites of the plasmid pET-3a under the control of the promoter of bacteriophage T7.
Proper citation: RRID:Addgene_17329 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1-T804N mScarlet-I donor
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: This plasmid can be used in combination with pX330_ATP1A1_G7 or eSpCas9(1.1)_No_FLAG_ATP1A1_G7 to target mScarlet-I to ATP1A1 intron 17. A user-specified transgene of interest can also be cloned using restriction cloning sites upstream (KpnI, AflII, NcoI) and downstream (XhoI, PmeI, SbfI) of the mSc-TOSI gene cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173207 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: ATP1A1 G7 sgRNA target ATP1A1 intron 17 and this plasmid can be used to perform coselection for HDR in human cells. This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 and user-specified sgRNA can be cloned using the BbsI sites. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173206 Copy
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