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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA Resource Report Resource Website |
RRID:Addgene_173213 | ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA | Homo sapiens | Ampicillin | PMID:36207338 | Control pegRNA vector derived from ATP1A1_G4_Q118R_Dual_pegRNA. This vector can be used as a positive control for prime editing (PE3 in combination with ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA) to co-target ATP1A1 and RUNX1 to perform coselection for RUNX1 +1 ATG insertion. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. | Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | |
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pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008) Resource Report Resource Website |
RRID:Addgene_173455 | mScarlet-mNeonGreen | Ampicillin | PMID:34985695 | Backbone Size:5614; Vector Backbone:pFA6a-mCherry-sfGFP-kanMX (pMaM17); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:32 | 0 | |||
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pM370 Resource Report Resource Website |
RRID:Addgene_173178 | gRNA-12xMBS | Synthetic | Ampicillin | PMID:32887690 | Backbone Size:7514; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | ||
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ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA Resource Report Resource Website |
RRID:Addgene_173211 | ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA | Homo sapiens | Ampicillin | PMID:36207338 | Control PE3 nick sgRNAs vector derived from ATP1A1_G3_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 and MTOR to perform coselection for MTOR hyperactivating mutation E2419K. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. | Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing (PE3); Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | |
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pBN089 Resource Report Resource Website |
RRID:Addgene_173451 | E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E | Escherichia coli | Ampicillin | PMID:34272394 | Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | K540M, Y717E, Y718E | 2026-08-15 01:10:32 | 0 | |
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pM355 Resource Report Resource Website |
RRID:Addgene_173175 | gRNA-12xMBS backbone | Synthetic | Ampicillin | PMID:32887690 | Backbone Marker:Promega; Backbone Size:4837; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | ||
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pBN088 Resource Report Resource Website |
RRID:Addgene_173450 | E. coli E69 (O9a:K30) Wzc_K540M with Y718E | Escherichia coli | Ampicillin | PMID:34272394 | Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | K540M, Y718E | 2026-08-15 01:10:32 | 0 | |
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pM80 Resource Report Resource Website |
RRID:Addgene_173173 | dCas9 | Synthetic | Ampicillin | PMID:32887690 | Backbone Size:6479; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | ||
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NHR1+2-Flag pcDNA3.1 Resource Report Resource Website |
RRID:Addgene_17316 | Neuralized-like | Mus musculus | Ampicillin | PMID:18077452 | Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 C-FLAG; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | ||
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Flag-NHR2+R pcDNA3.1 Resource Report Resource Website |
RRID:Addgene_17317 | Neuralized-like | Mus musculus | Ampicillin | PMID:18077452 | Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | ||
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Neurl1G2A-Flag pcDNA3.1 Resource Report Resource Website |
RRID:Addgene_17320 | Neuralized-like | Mus musculus | Ampicillin | PMID:18077452 | Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 C-FLAG; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin | Gly 2 changed to Ala | 2026-08-15 01:10:31 | 0 | |
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2xHA MED10 pBacPAK8 Resource Report Resource Website |
RRID:Addgene_17321 | MED10 | Mus musculus | Ampicillin | PMID:18077452 | The Med10 sequence was inserted between XhoI and Kpn1 of pBacPAK8 polylinker after changing the original BacPAK8 BamHI site to NheI. Plasmid can be used for Med10 expression or, by cutting out and replacing Med10 CDS with something else, for construction of BacPAK8 derivatives expressing 2xHA-tagged proteins. | Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | |
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NHR1+2-Flag pBacPAK8-3 Resource Report Resource Website |
RRID:Addgene_17324 | Neuralized-like | Mus musculus | Ampicillin | PMID:18077452 | Backbone Marker:Conaway Lab; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | ||
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pTSA-13 Resource Report Resource Website 1+ mentions |
RRID:Addgene_17327 | streptavidin | Streptomyces avidinii | Ampicillin | PMID:7499314 | Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Truncated to include only amino acids 16-133 of mature streptavidin | 2026-08-15 01:10:31 | 2 | |
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ATP1A1_G4_Q118R_Dual_pegRNA Resource Report Resource Website |
RRID:Addgene_173199 | ATP1A1 G4 Q118R pegRNA | Homo sapiens | Ampicillin | PMID:36207338 | Prime editing vector derived from pU6-pegRNA-GG-acceptor. See Anzalone et al. Nature (2019) for the detailed pegRNA cloning protocol to target your gene of interest. False-positive colonies will appear pink or red if the RFP insert has not been replaced by the new pegRNA cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. | Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | |
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pTSA-38 Resource Report Resource Website |
RRID:Addgene_17328 | streptavidin | Streptomyces avidinii | Ampicillin | PMID:7724536 | pTSA-38 is a derivative of pTSA-13 with a W120F mutation. In brief, mpSA-29 was generated by inserting a 480-bp fragment of pTSA-13 carrying the entire coding sequence into M13mpl8. Oligonucleotide-directed in vitro mutagenesis was performed on mpSA-29 by using the 30-nt oligonucleotide, 5'-ACCAGCGTGGACTTGAAG- GCGTTGGCCTCG-3', to convert the TGG codon for Trp- 120 to TTC for Phe (mpSA-36). Then, a 405-bp fragment of mpSA-36 carrying the entire coding sequence was cloned into pET-3a. The resulting expression vector, pTSA-38, encodes a streptavidin W120F mutant. | Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Truncated to include only amino acids 16-133 of mature streptavidin. Trp-120 is replaced by Phe. | 2026-08-15 01:10:31 | 0 |
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pAAV-hSyn-Mito-HyPerGRt Resource Report Resource Website |
RRID:Addgene_173198 | Hyper7-tdTomato | Synthetic | Ampicillin | PMID:35226474 | Backbone Size:4268; Vector Backbone:pAAV-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | ||
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pTSA-C Resource Report Resource Website 1+ mentions |
RRID:Addgene_17329 | streptavidin | Streptomyces avidinii | Ampicillin | PMID:11716692 | A PCR-based site-directed mutagenesis method was used to modify the coding sequence of pTSA-13. A DNA sequence encoding six amino acid residues with a translation termination codon, GGT GGT TCT GGT TGC CCG TAG (Gly-Gly-Ser-Gly-Cys-Pro-Stop), was fused to the codon for Val-133 (GTG). The entire coding sequence was cloned between the NdeI and BamHI sites of the plasmid pET-3a under the control of the promoter of bacteriophage T7. | Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Truncated to include only amino acids 16-133 of mature streptavidin. Following Val133, a six-residue immobilization sequence (Gly-Gly-Ser-Gly-Cys-Pro) was added. | 2026-08-15 01:10:31 | 2 |
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ATP1A1_T804N_hPGK1_mScarlet-I_Donor Resource Report Resource Website |
RRID:Addgene_173207 | ATP1A1-T804N mScarlet-I donor | Homo sapiens | Ampicillin | PMID:36207338 | This plasmid can be used in combination with pX330_ATP1A1_G7 or eSpCas9(1.1)_No_FLAG_ATP1A1_G7 to target mScarlet-I to ATP1A1 intron 17. A user-specified transgene of interest can also be cloned using restriction cloning sites upstream (KpnI, AflII, NcoI) and downstream (XhoI, PmeI, SbfI) of the mSc-TOSI gene cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. | Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 | |
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pX330_ATP1A1_G7_Dual_sgRNA Resource Report Resource Website |
RRID:Addgene_173206 | ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9 | Homo sapiens | Ampicillin | PMID:36207338 | ATP1A1 G7 sgRNA target ATP1A1 intron 17 and this plasmid can be used to perform coselection for HDR in human cells. This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 and user-specified sgRNA can be cloned using the BbsI sites. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. | Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:31 | 0 |
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