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Species: Other
Genetic Insert: Hyg
Vector Backbone Description: Vector Backbone:pAG32; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24625580
Proper citation: RRID:Addgene_87251 Copy
Species: Synthetic
Genetic Insert: YFP-mAID-3HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2925; Vector Backbone:pBluescript SK(+); Vector Types:Toxoplasma expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28465425
Proper citation: RRID:Addgene_87259 Copy
Species: Homo sapiens
Genetic Insert: NUP93
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4761; Vector Backbone:pEGFP-C1X; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27613095
Proper citation: RRID:Addgene_87336 Copy
Genetic Insert: Cas9-2A-eGFP
Vector Backbone Description: Backbone Marker:Feng Zhang lab; Backbone Size:16733; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Doxycycline inducible; eGFP reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30795624
Comments: Clone sgRNAs into TLCV2 using the same protocol provided with lentiCRISPR v2 (Plasmid #52961, protocol listed under the RESOURCE INFORMATION section). After you have inserted your sgRNA, you should sequence this region with one of the following primers: hU6-F (5'-GAGGGCCTATTTCCCATGATT-3') or LKO.1 5’(5'- GACTATCATATGCTTACCGT-3').
Proper citation: RRID:Addgene_87360 Copy
Genetic Insert: yEGFP
Vector Backbone Description: Backbone Size:4738; Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15197731
Comments: Full name of this plasmid is pFA6a-link-yEGFP-SpHIS5. An codon-optimized linker was added into the yEGFP to improve expression level. The selectable marker, Sz. pombe HIS5 (SpHIS5), complements S. cerevisiae HIS3.
The yeast GFP used to create this plasmid contained a point mutation M233I that is present in all GFP variants derived from this original yEGFP. The mutation had no effect on fluorescence (see associated publication for more details).
Proper citation: RRID:Addgene_8729 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26524520
Proper citation: RRID:Addgene_87350 Copy
Species: Synthetic
Genetic Insert: Three-way junction dimeric Broccoli aptamer
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87308 Copy
Genetic Insert: yECitrine
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15197731
Comments: Full name of this plasmid is pFA6a-link-yECitrine-KanR. yECitrine is created by mutagenesis from a codon-optimized green fluorescent protein - yEGFP1 (Cormack et al. 1997). This yEGFP variant is cloned into pDH5, replacing YFP. An codon-optimized linker was added into the yEGFP variant to improve expression level. The G418 resistance marker (KanR) was introduced into the plasmid by subcloning the BglII/EcoRI fragment of pDH3 (Hailey et al., 2002) into the plasmid, in place of SpHIS5.
The yeast GFP used to create this plasmid contained a point mutation M233I that is present in all GFP variants derived from this original yEGFP. The mutation had no effect on fluorescence (see associated publication for more details).
Proper citation: RRID:Addgene_8732 Copy
Genetic Insert: yEGFP
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15197731
Comments: Full name of this plasmid is pFA6a-link-yEGFP-CaURA3. An codon-optimized linker was added into the yEGFP to improve expression level. C. albicans URA3 (CaURA3) was introduced by subcloning the BglII/SacI fragment of pAG60 (Goldstein et al., 1999) into the plasmid, in place of SpHIS5.
The yeast GFP used to create this plasmid contained a point mutation M233I that is present in all GFP variants derived from this original yEGFP. The mutation had no effect on fluorescence (see associated publication for more details).
Proper citation: RRID:Addgene_8730 Copy
Species: Mus musculus
Genetic Insert: Bcl-2
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pSFFV-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8358790
Proper citation: RRID:Addgene_8750 Copy
Species: Homo sapiens
Genetic Insert: PCRD (proximal C-terminal regulatory domain)
Vector Backbone Description: Backbone Size:5382; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28059704
Comments: FKBP (324 bp) (protein ID AAA58472)
Proper citation: RRID:Addgene_87452 Copy
Species: Homo sapiens
Genetic Insert: FBL Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28814507
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human FBL in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: KPNSAVDGTAGPG. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Please visit https://www.biorxiv.org/content/early/2017/03/31/123042 for bioRxiv preprint.
Proper citation: RRID:Addgene_87427 Copy
Species: Homo sapiens
Genetic Insert: TOMM20 Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28814507
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human TOMM20 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGDPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Please visit https://www.biorxiv.org/content/early/2017/03/31/123042 for bioRxiv preprint.
Proper citation: RRID:Addgene_87423 Copy
Species: Synthetic
Genetic Insert: FlpO aa397-423
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28346402
Proper citation: RRID:Addgene_87560 Copy
Species: Synthetic
Genetic Insert: FlpO aa1-396
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28346402
Proper citation: RRID:Addgene_87559 Copy
Species: Homo sapiens
Genetic Insert: Bcl-2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2700; Vector Backbone:pGEM-4Z; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8183370
Proper citation: RRID:Addgene_8751 Copy
Species: Mus musculus
Genetic Insert: Bcl-XL (Exon 1)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7650488
Proper citation: RRID:Addgene_8755 Copy
Species: Mus musculus
Genetic Insert: Bax
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7569956
Comments: for in situ probes: antisense - cut with PstI and use T7 polymerase. sense - cut with SalI and use T3 polymerase.
Proper citation: RRID:Addgene_8754 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28346402
Proper citation: RRID:Addgene_87554 Copy
Species: Mycobacterium phage Bxb1
Genetic Insert: Bxb1 attP
Vector Backbone Description: Vector Backbone:pBluescript SK+; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28118814
Proper citation: RRID:Addgene_87629 Copy
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