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Species: Soybean (Glyma.18G281500.1.p)
Genetic Insert: ID#84
Vector Backbone Description: Vector Backbone:pTWIST ENTRY; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37248633
Proper citation: RRID:Addgene_193535 Copy
Species: Brachypodium (Bradi5g22187.1.p)
Genetic Insert: ID#65
Vector Backbone Description: Vector Backbone:pTWIST ENTRY; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37248633
Proper citation: RRID:Addgene_193532 Copy
Species: Aequorea victoria
Genetic Insert: bleach.3
Vector Backbone Description: Backbone Size:5209; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37210560
Comments: Design created by the htFuncLib algorithm
Proper citation: RRID:Addgene_191914 Copy
Species: Aequorea victoria
Genetic Insert: mid.12
Vector Backbone Description: Backbone Size:5209; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37210560
Comments: Design created by the htFuncLib algorithm
Proper citation: RRID:Addgene_191913 Copy
Species: Medicago (Medtr5g018110.1)
Genetic Insert: ID#39
Vector Backbone Description: Vector Backbone:pTWIST ENTRY; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37248633
Proper citation: RRID:Addgene_193539 Copy
Species: Soybean (Glyma.18G234500.1.p)
Genetic Insert: ID#87
Vector Backbone Description: Vector Backbone:pTWIST ENTRY; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37248633
Proper citation: RRID:Addgene_193549 Copy
Species: Tomato (Solyc05g007170.2.1)
Genetic Insert: ID#22
Vector Backbone Description: Vector Backbone:pTWIST ENTRY; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37248633
Proper citation: RRID:Addgene_193542 Copy
Species: Brachypodium (Bradi4g09648.1.p)
Genetic Insert: ID#57
Vector Backbone Description: Vector Backbone:pTWIST ENTRY; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37248633
Proper citation: RRID:Addgene_193543 Copy
Genetic Insert: NTERY07
Vector Backbone Description: Vector Backbone:pCDB180; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34385692
Proper citation: RRID:Addgene_184125 Copy
Genetic Insert: NTERY04
Vector Backbone Description: Vector Backbone:pCDB180; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34385692
Proper citation: RRID:Addgene_184122 Copy
Species: Homo sapiens
Genetic Insert: SHIP1
Vector Backbone Description: Backbone Marker:Novagen-EMD Millipore; Backbone Size:5309; Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35603158
Proper citation: RRID:Addgene_183770 Copy
Species: Synthetic
Genetic Insert: AviTag-SpyTag003-MBP
Vector Backbone Description: Backbone Size:5219; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35764623
Proper citation: RRID:Addgene_184227 Copy
Species: Homo sapiens
Genetic Insert: SHIP1deltaC2
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6067; Vector Backbone:pKan S97Split; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35603158
Proper citation: RRID:Addgene_183769 Copy
Species: Homo sapiens
Genetic Insert: SLC35A2
Vector Backbone Description: Vector Backbone:pEGFP.N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30834435
Proper citation: RRID:Addgene_186285 Copy
Species: Homo sapiens
Genetic Insert: TTR
Vector Backbone Description: Backbone Marker:emdmillipore; Backbone Size:5371; Vector Backbone:pet29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33645214
Proper citation: RRID:Addgene_184748 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-GFP-Rta
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183930 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-GFP-STAT2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183931 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*57:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00381 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180444 Copy
Species: Escherichia coli K12 MG1655
Genetic Insert: araC
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pNB101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35798732
Comments: Plasmid design adapted from Daniel R, Rubens JR, Sarpeshkar R, Lu TK. Synthetic analog computation in living cells. Nature. 2013 May;497(7451):619–23.
Please visit https://doi.org/10.1101/2021.03.26.437171 for bioRxiv preprint.
Proper citation: RRID:Addgene_169110 Copy
Species: Staphylococcus aureus
Genetic Insert: SaCas9
Vector Backbone Description: Backbone Marker:Addgene, #141322; Backbone Size:5198; Vector Backbone:pET-28b-RfxCas13d-His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35403007
Proper citation: RRID:Addgene_178901 Copy
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