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Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y715E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173448 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: ATP1A1 G3 sgRNA target ATP1A1 intron 4 and this plasmid can be used to perform coselection for HDR in human cells. This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 and user-specified sgRNA can be cloned using the BbsI sites. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173205 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y713E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173447 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G7 sgRNA + SpCas9
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: ATP1A1 G7 sgRNA target ATP1A1 intron 17 and this plasmid can be used to directly target a transgene to the ATP1A1 locus itselft, including the mTOR Signaling Indicator (TOSI) using ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor.
This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173204 Copy
Species: Homo sapiens
Genetic Insert: Glycerol-3-Phosphate Acyltransferase 4
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30846317
Proper citation: RRID:Addgene_173168 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G2 sgRNA + user-specified sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: U6-sgRNA cassette derived from pX330-U6-Chimeric_BB-CBh-hSpCas9. New user-specified sgRNA can be cloned using the BbsI sites. This vector can be used for adenine base editing or NHEJ-based editing to co-target ATP1A1 and a gene of interest. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173201 Copy
Genetic Insert: mNeonGreen-CBP-2XFLAG-HYGB
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34791226
Proper citation: RRID:Addgene_173442 Copy
Species: Homo sapiens
Genetic Insert: Calcineurin Like EF-Hand Protein 1
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30846317
Proper citation: RRID:Addgene_173166 Copy
Genetic Insert: mNeonGreen-CBP-2XFLAG-NAT1
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34791226
Proper citation: RRID:Addgene_173441 Copy
Species: Homo sapiens
Genetic Insert: KCNQ3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5130; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35104249
Proper citation: RRID:Addgene_173163 Copy
Species: Mus musculus
Genetic Insert: AP1M1
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24229647
Proper citation: RRID:Addgene_173432 Copy
Species: Homo sapiens
Genetic Insert: LgBiT-hACE2-IgG1 Fc
Vector Backbone Description: Backbone Size:4200; Vector Backbone:phCMV3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34183368
Proper citation: RRID:Addgene_173431 Copy
Species: Homo sapiens
Genetic Insert: KCNQ2
Vector Backbone Description: Backbone Size:5671; Vector Backbone:pCS2_IRES2_CyOFP1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33544076
Proper citation: RRID:Addgene_173155 Copy
Species: Mus musculus
Genetic Insert: ORF clone Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1)
Vector Backbone Description: Backbone Marker:Genescript; Backbone Size:5432; Vector Backbone:pcDNA3.1(+)-C-6His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39032642
Comments: Please visit https://doi.org/10.1101/2021.11.16.468891 for bioRxiv preprint.
Proper citation: RRID:Addgene_173153 Copy
Species: Phytophthora infestans
Genetic Insert: PITG_00582_T30-4
Vector Backbone Description: Backbone Marker:Youles SynBio plasmids; Backbone Size:8678; Vector Backbone:pICSL86977OD; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34424903
Proper citation: RRID:Addgene_173506 Copy
Species: Aedes albopictus
Genetic Insert: Nix masculinisation gene
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35256751
Comments: The OpIE2-GFP transgenesis marker may become detectable at late stages of mosquito development.
Note that the plasmid sequence spanning Nix was assembled from database sequence. The actual sequence in the plasmid may contain differences due to natural polymorphism, especially in the Nix promoter
Proper citation: RRID:Addgene_173505 Copy
Species: Homo sapiens
Genetic Insert: PGRMC2
Vector Backbone Description: Vector Backbone:pCMV-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34645803
Proper citation: RRID:Addgene_173468 Copy
Species: Homo sapiens
Genetic Insert: PGRMC1
Vector Backbone Description: Vector Backbone:pCMV-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34645803
Proper citation: RRID:Addgene_173467 Copy
Species: Phytophthora infestans
Genetic Insert: PITG_04085_T30-4
Vector Backbone Description: Backbone Marker:Youles SynBio plasmids; Backbone Size:8678; Vector Backbone:pICSL86977OD; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34424903
Proper citation: RRID:Addgene_173509 Copy
Species: Phytophthora infestans
Genetic Insert: PITG_02860_T30-4
Vector Backbone Description: Backbone Marker:Youles SynBio plasmids; Backbone Size:8678; Vector Backbone:pICSL86977OD; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34424903
Proper citation: RRID:Addgene_173508 Copy
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