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Species: Homo sapiens
Genetic Insert: MNTS-1-p75
Vector Backbone Description: Backbone Marker:Pantelis Tsoulfas; Backbone Size:6756; Vector Backbone:pLV-MP2; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7529173
Proper citation: RRID:Addgene_182945 Copy
Species: Homo sapiens
Genetic Insert: Lamin A
Vector Backbone Description: Backbone Size:8589; Vector Backbone:pLenti Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124521
Comments: Addgene QC NGS analysis returned BLAST results with significant sequence identity to Lamin A/C.
Proper citation: RRID:Addgene_182673 Copy
Species: Homo sapiens
Genetic Insert: IGLC2
Vector Backbone Description: Backbone Marker:adapted from Burton lab; Backbone Size:5000; Vector Backbone:AbVec; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_183703 Copy
Species: Homo sapiens
Genetic Insert: IGHG1
Vector Backbone Description: Backbone Marker:adapted from Burton lab; Backbone Size:5000; Vector Backbone:AbVec; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_183701 Copy
Species: Homo sapiens
Genetic Insert: U1C
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9724750
Comments: (Leder #C-1090)
Proper citation: RRID:Addgene_1840 Copy
Species: Homo sapiens
Genetic Insert: SmB'
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9724750
Comments: (Leder #C-1092)
Proper citation: RRID:Addgene_1842 Copy
Species: Homo sapiens
Genetic Insert: thioredoxin-like 4B
Vector Backbone Description: Backbone Size:2930; Vector Backbone:pBZ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18064608
Comments: attB cassette flanking coding region of TXNL4B (dim2) can be released by digestion with NotI and BamHI
Proper citation: RRID:Addgene_18667 Copy
Species: Homo sapiens
Genetic Insert: Bicistronic fluorescent reporter gene with cap-dependent EYFP and IRES-dependent ECFP translation under the control of CMV promo
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17023487
Comments: EYFP tagged at N-terminus with 3 copies of Myc epitope tags and at C-terminus with a copy of FLAG and polyhistidine (His6) epitope tags. ECFP tagged at the C-terminus with one copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags.
EGFP of pIRES2-EGFP (Clontech) replaced at an 803 bp BstXI-NotI, PCR amplified fragment with ECFP from pECFP-Mito (Clontech) tagged at the C-terminus with a single copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags; IRES-dependent translation yields ECFP-HA-His6 fusion protein. 895 bp Sac I/Eco RI fragment containing amino terminal 3 Myc epitope tags fused to EYFP obtained from PCR amplification of pEYFP-N1 (Clontech), which is also tagged at C-terminus with a single copy of FLAG and polyhistidine (His6) epitope tag; cap-dependent translation yields 3Myc-EYFP-Flag-His6 fusion protein.
See article for detailed information.
Proper citation: RRID:Addgene_18673 Copy
Species: Homo sapiens
Genetic Insert: MDM2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS314 w/Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8479525
Proper citation: RRID:Addgene_18654 Copy
Species: Homo sapiens
Genetic Insert: ubiquitin
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9353319
Comments: This plasmid grows slowly. We encourage requesting scientists to grow for a longer period of time than normal. Please note that this plasmid runs as a dimer (>10kb) or multimer.
Proper citation: RRID:Addgene_18712 Copy
Species: Homo sapiens
Genetic Insert: E-cadherin shRNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1 hygro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18483246
Comments: siRNA directed against E-cadherin: 5'-AAGATAGGAGTTCTCTGATGC-3'. Sequence from the website of the RNAi consortium at the Broad institute.
Please note that the sequence originally published in the paper was incorrect and the sequence above is the actual shRNA used.
Proper citation: RRID:Addgene_18802 Copy
Species: Homo sapiens
Genetic Insert: b-catenin shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18483246
Comments: siRNA directed against beta-catenin: 5'-GCTTGGAATGAGACTGCTGAT-3'. Sequence from the website of the RNAi consortium at the Broad institute.
Proper citation: RRID:Addgene_18803 Copy
Species: Homo sapiens
Genetic Insert: myc T58A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16094360
Proper citation: RRID:Addgene_18773 Copy
Species: Homo sapiens
Genetic Insert: H-Ras V12
Vector Backbone Description: Backbone Marker:Jay Morgenstern; Backbone Size:5763; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9054499
Proper citation: RRID:Addgene_18741 Copy
Species: Homo sapiens
Genetic Insert: H-Ras
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18744 Copy
Species: Homo sapiens
Genetic Insert: H-Ras V12 S35
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18746 Copy
Species: Homo sapiens
Genetic Insert: H-Ras V12 C40
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18747 Copy
Species: Homo sapiens
Genetic Insert: Yap1 Y357E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18280240
Proper citation: RRID:Addgene_18883 Copy
Species: Homo sapiens
Genetic Insert: LIN28
Vector Backbone Description: Backbone Size:10000; Vector Backbone:EGIP; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18511599
Proper citation: RRID:Addgene_18921 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin conjugating enzyme E2-25K
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pET3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15917625
Proper citation: RRID:Addgene_18892 Copy
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