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Species: Synthetic
Genetic Insert: moxGFP-P2A-T2A
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5857; Vector Backbone:pSBbi-Pur (Addgene Plasmid #60523); Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34877484
Comments: The moxGFP-P2A-T2A construct was designed by Jorge L. Arias-Arias from Universidad de Costa Rica ([email protected]). It was codon optimized for human and mouse expression and includes two additional cloning sites (5' EcoRV - 3' SalI and 5' SmaI - 3' XbaI, see the map uploaded by the depositor) for the polycistronic expression of up to three genes: moxGFP and two more. This vector is suitable for transient transfection, but it was intended to be co-transfected with the transposase vector pCMV(CAT)T7-SB100 (Addgene Plasmid #34879) in order to generate mammalian cells with stable expression of the genes of interest. Enjoy!
Proper citation: RRID:Addgene_176893 Copy
Species: Homo sapiens
Genetic Insert: Granulin 7
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28828399
Proper citation: RRID:Addgene_176931 Copy
Species: Mus musculus
Genetic Insert: Integrin-linked kinase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35259013
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437490v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176897 Copy
Species: Homo sapiens
Genetic Insert: Granulin 7+linker8
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28828399
Proper citation: RRID:Addgene_176930 Copy
Species: Homo sapiens
Genetic Insert: Granulin 3
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28828399
Proper citation: RRID:Addgene_176923 Copy
Species: Homo sapiens
Genetic Insert: Granulin 6+linker7
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28828399
Proper citation: RRID:Addgene_176928 Copy
Species: Homo sapiens
Genetic Insert: Granulin 5
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28828399
Proper citation: RRID:Addgene_176927 Copy
Species: Homo sapiens
Genetic Insert: RWDD2B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: RWDD2B (NM_016940) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176956 Copy
Species: Homo sapiens
Genetic Insert: PCBP3
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: RRP1 (BC012061) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176958 Copy
Species: Homo sapiens
Genetic Insert: CRYZL1
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Proper citation: RRID:Addgene_177004 Copy
Species: Homo sapiens
Genetic Insert: SLC19A1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: SLC19A1 (BC003068) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176951 Copy
Species: Homo sapiens
Genetic Insert: B3GALT5
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Proper citation: RRID:Addgene_177006 Copy
Species: Homo sapiens
Genetic Insert: FAM207A
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: FAM207A (NM_058190) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176950 Copy
Species: Homo sapiens
Genetic Insert: ETS2
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Proper citation: RRID:Addgene_177005 Copy
Species: Mus musculus
Genetic Insert: Rcan1
Vector Backbone Description: Backbone Marker:Derived from Addgene #107398; KanR replaced with AmpR; Backbone Size:9341; Vector Backbone:plenti-CAG-gate-FLAG-IRES-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Proper citation: RRID:Addgene_177000 Copy
Species: Homo sapiens
Genetic Insert: TMEM50B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: TMEM50B (NM_006134) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176940 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: 8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: KF302069, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177023 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: iridoid oxidase; CYP76A26
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: KF302066, amplified from cDNA, contains T359S relative to KF302066, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177026 Copy
Species: Nepeta mussinii (aka Nepeta racemosa)
Genetic Insert: major latex protein-like
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: MT108267.1, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177025 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: 7-deoxyloganetic acid glucosyl transferase; UGT709C2
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: KF302068, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177027 Copy
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