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Species: Homo sapiens
Genetic Insert: SCARB1
Vector Backbone Description: Vector Backbone:pDUAL GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Depositor notes that S97F mutation in SCARB1 insert does not affect function.
Proper citation: RRID:Addgene_86979 Copy
Species: Staphylococcus aureus
Genetic Insert: S.aureus SrtA 5M
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28088451
Comments: Pentamutant sortase A was originally described by David R. Liu's laboratory (Harvard University) in: A General Strategy for the Evolution of Bond-Forming Enzymes Using Yeast Display. Chen I, Dorr BM, Liu DR. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404 (PubMed ID: 21697512). As noted in this publication, the cloned sortase A gene lacks the N-terminal 59 amino acids, which do not impact catalytic activity, but these amino acids are still included in the numbering for the mutations.
Proper citation: RRID:Addgene_86962 Copy
Species: Drosophila melanogaster
Genetic Insert: H3
Vector Backbone Description: Backbone Size:10200; Vector Backbone:na; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12086617
Comments: Heat shock promoter. Ahmad lab #k90.
Proper citation: RRID:Addgene_8698 Copy
Species: S. pyogenes
Genetic Insert: SpCas9-VQR(D1135V,R1335Q,T1337R)
Vector Backbone Description: Vector Backbone:lentiCas9-Blast; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28218758
Proper citation: RRID:Addgene_87155 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_87067 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_87065 Copy
Species: Homo sapiens
Genetic Insert: Spinophilin
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Donor vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28634551
Proper citation: RRID:Addgene_87123 Copy
Species: Synthetic
Genetic Insert: U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA
Vector Backbone Description: Backbone Marker:Addgene 60957; Vector Backbone:PX551; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27851729
Comments: There is 11bp deletion in left ITR, which is from original backbone plasmid (i.e. PX551). We don't believe it interfered with packaging.
Proper citation: RRID:Addgene_87119 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25036637
Proper citation: RRID:Addgene_87071 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Slow-growing, requires 3 days growth at 30C
Proper citation: RRID:Addgene_87075 Copy
Species: Synthetic
Genetic Insert: U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
Vector Backbone Description: Backbone Marker:Addgene 60957; Vector Backbone:PX551; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27851729
Comments: There is 11bp deletion in left ITR, which is from original backbone plasmid (i.e. PX551). We don't believe it interfered with packaging.
Proper citation: RRID:Addgene_87116 Copy
Species: Homo sapiens
Genetic Insert: 1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
Vector Backbone Description: Backbone Marker:SBI System Biosciences; Vector Backbone:PBHR100A-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28238795
Proper citation: RRID:Addgene_87228 Copy
Species: Other
Genetic Insert: EnvA-VSVg
Vector Backbone Description: Backbone Size:5302; Vector Backbone:pCAGGS/ES; Vector Types:Mammalian Expression, Mouse Targeting, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27974160
Proper citation: RRID:Addgene_87221 Copy
Genetic Insert: yEGFP
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15197731
Comments: Full name of this plasmid is pFA6a-link-yEGFP-KanR. An codon-optimized linker was added into the yEGFP to improve expression level. The G418 resistance marker (KanR) was introduced into the plasmid by subcloning the BglII/EcoRI fragment of pDH3 (Hailey et al., 2002) into the plasmid, in place of SpHIS5.
The yeast GFP used to create this plasmid contained a point mutation M233I that is present in all GFP variants derived from this original yEGFP. The mutation had no effect on fluorescence (see associated publication for more details).
Proper citation: RRID:Addgene_8728 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 1 alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5433; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17220275
Comments: pcDNA3.0-HA-HIF1α P402A/P564A/N803A was made from pcDNA3.0-HA-HIF1α P402A/P564A ( Addgene (Plasmid #18955) using the QuikChange site-directed mutagenesis kit (Stratagene)
Proper citation: RRID:Addgene_87261 Copy
Species: Other
Genetic Insert: Hyg
Vector Backbone Description: Vector Backbone:pAG32; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24625580
Proper citation: RRID:Addgene_87251 Copy
Species: Synthetic
Genetic Insert: YFP-mAID-3HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2925; Vector Backbone:pBluescript SK(+); Vector Types:Toxoplasma expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28465425
Proper citation: RRID:Addgene_87259 Copy
Species: Homo sapiens
Genetic Insert: NUP93
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4761; Vector Backbone:pEGFP-C1X; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27613095
Proper citation: RRID:Addgene_87336 Copy
Genetic Insert: Cas9-2A-eGFP
Vector Backbone Description: Backbone Marker:Feng Zhang lab; Backbone Size:16733; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Doxycycline inducible; eGFP reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30795624
Comments: Clone sgRNAs into TLCV2 using the same protocol provided with lentiCRISPR v2 (Plasmid #52961, protocol listed under the RESOURCE INFORMATION section). After you have inserted your sgRNA, you should sequence this region with one of the following primers: hU6-F (5'-GAGGGCCTATTTCCCATGATT-3') or LKO.1 5’(5'- GACTATCATATGCTTACCGT-3').
Proper citation: RRID:Addgene_87360 Copy
Genetic Insert: yEGFP
Vector Backbone Description: Backbone Size:4738; Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15197731
Comments: Full name of this plasmid is pFA6a-link-yEGFP-SpHIS5. An codon-optimized linker was added into the yEGFP to improve expression level. The selectable marker, Sz. pombe HIS5 (SpHIS5), complements S. cerevisiae HIS3.
The yeast GFP used to create this plasmid contained a point mutation M233I that is present in all GFP variants derived from this original yEGFP. The mutation had no effect on fluorescence (see associated publication for more details).
Proper citation: RRID:Addgene_8729 Copy
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