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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pRSpuro shID1#6 Resource Report Resource Website |
RRID:Addgene_19165 | small hairpin RNA against inhibitor of DNA binding 1 | Homo sapiens | Ampicillin | PMID:18048329 | See author's map for sequence details | Backbone Size:6400; Vector Backbone:pRetroSuper-Puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:42 | 0 | |
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HA-HIF1alpha-wt-pBabe-puro Resource Report Resource Website 10+ mentions |
RRID:Addgene_19365 | Hypoxia inducible factor 1 alpha | Homo sapiens | Ampicillin | PMID:17220275 | Backbone Size:5188; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | wt human cDNA | 2026-08-15 01:11:44 | 13 | |
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Flag pLJM1 RagB 54L Resource Report Resource Website 1+ mentions |
RRID:Addgene_19314 | Ras-related GTP binding B | Homo sapiens | Ampicillin | PMID:18497260 | pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI Note from the depositor: This plasmid does not express well. This is because it is a dominant negative and therefore inhibits the mTORC1 pathway and thus cells. | Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | T54L | 2026-08-15 01:11:43 | 2 |
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Flag pLJM1 RagD 77L Resource Report Resource Website 1+ mentions |
RRID:Addgene_19317 | Ras-related GTP binding D | Homo sapiens | Ampicillin | PMID:18497260 | pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI | Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | S77L | 2026-08-15 01:11:44 | 3 |
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Flag pLJM1 RagD wt Resource Report Resource Website 1+ mentions |
RRID:Addgene_19316 | Ras-related GTP binding D | Homo sapiens | Ampicillin | PMID:18497260 | pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI | Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:43 | 4 | |
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pRK5-HA GST RagB 54L Resource Report Resource Website 1+ mentions |
RRID:Addgene_19302 | Ras-related GTP binding B | Homo sapiens | Ampicillin | PMID:18497260 | Please note that this plasmid is actually quite toxic to cells. From the Sabatini lab:"it is a dominant negative and therefore inhibits the mTORC1 pathway and thus cells." From the paper: "To verify the actions of the Rags in a more physiological setting than that achieved by transient cDNA transfection, we generated HEK-293T cell lines stably expressing Rheb1, RagB, or RagBGTP (attempts to generate lines stably expressing RagBGDP failed)." | Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | T54L | 2026-08-15 01:11:43 | 8 |
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pRK5-HA GST RagB wt Resource Report Resource Website 10+ mentions |
RRID:Addgene_19301 | Ras-related GTP binding B | Homo sapiens | Ampicillin | PMID:18497260 | Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:43 | 14 | ||
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pRK5-HA GST RagC 75L Resource Report Resource Website 10+ mentions |
RRID:Addgene_19305 | Ras-related GTP binding C | Homo sapiens | Ampicillin | PMID:18497260 | Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S75L | 2026-08-15 01:11:43 | 22 | |
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pRK5-HA GST RagD 77L Resource Report Resource Website 10+ mentions |
RRID:Addgene_19308 | Ras-related GTP binding D | Homo sapiens | Ampicillin | PMID:18497260 | Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S77L | 2026-08-15 01:11:43 | 10 | |
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pcDNA5/FRT/TO V5 HSPA1L Resource Report Resource Website |
RRID:Addgene_19511 | heat shock 70kDa protein 1-like | Homo sapiens | Ampicillin | PMID:18686016 | Part of the HSP70 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene. | Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:45 | 0 | |
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pcDNA5/FRT/TO V5 HSPA8 Resource Report Resource Website 10+ mentions |
RRID:Addgene_19514 | heat shock 70kDa protein 8 | Homo sapiens | Ampicillin | PMID:18686016 | Part of the HSP70 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene. | Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:45 | 11 | |
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pcDNA5/FRT/TO V5 DNAJB2b Resource Report Resource Website |
RRID:Addgene_19524 | DNAJB2b | Homo sapiens | Ampicillin | PMID:18686016 | Part of the HSP40 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene. | Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:46 | 0 | |
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pcDNA5/FRT/TO DNAJA4 Resource Report Resource Website |
RRID:Addgene_19467 | DNAJA4 | Homo sapiens | Ampicillin | PMID:18686016 | Family: HSP40 Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene. | Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:45 | 0 | |
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pcDNA3-HA-Uch37 Resource Report Resource Website 1+ mentions |
RRID:Addgene_19415 | Uch37 | Homo sapiens | Ampicillin | PMID:16906146 | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:44 | 5 | ||
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pcDNA5-Flag-Adrm1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_19417 | adhesion regulating molecule 1 | Homo sapiens | Ampicillin | PMID:16906146 | Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:45 | 4 | ||
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pBABE Gal4 (1-94) HSF ABC Flag wt Resource Report Resource Website |
RRID:Addgene_1945 | Heat Shock Factor 1 | Homo sapiens | Ampicillin | PMID:11486022 | contains the FLAG epitope amino terminal to the GAL4 DNA binding domain (amino acids 1 to 94), attached to the C terminus of hHSF1, (amino acids 201 to 529). This construct was subcloned into the pBABE/puro retroviral vector via the BglII and EcoRI sites. (Kingston #1152) | Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:45 | 0 | |
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pBABE Gal4(1-94) HSF ABC Flag ac Resource Report Resource Website |
RRID:Addgene_1946 | Heat Shock Factor 1 | Homo sapiens | Ampicillin | PMID:11486022 | contains the FLAG epitope amino terminal to the GAL4 DNA binding domain (amino acids 1 to 94), attached to the C terminus of hHSF1, (amino acids 201 to 529). This construct was subcloned into the pBABE/puro retroviral vector via the BglII and EcoRI sites. Acidic Mutant. (Kingston #1153) | Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | acidic mutant (D416, E493, E496 A) | 2026-08-15 01:11:45 | 0 |
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pBABE HSF Flag wt Resource Report Resource Website 1+ mentions |
RRID:Addgene_1948 | Heat Shock Factor 1 | Homo sapiens | Ampicillin | PMID:11486022 | FLAG-tagged full length hHSF1 (aa1-529) was generated by subcloning the human HSF1-flag construct out of pCMV-HSF1-flag and into the pBABE/puro retroviral vector via the EcoRI site. (Kingston #1155) | Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:45 | 4 | |
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pBABE HSF Flag Ac Resource Report Resource Website |
RRID:Addgene_1949 | Heat Shock Factor 1 | Homo sapiens | Ampicillin | PMID:11486022 | FLAG-tagged full length hHSF1 (aa1-529) was generated by subcloning the human HSF1-flag construct out of pCMV-HSF1-flag and into the pBABE/puro retroviral vector via the EcoRI site. Acidic Mutant. (Kingston #1156) | Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Acidic mutations (D416, E493, E496 A) | 2026-08-15 01:11:45 | 0 |
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BacPAK Myc-Adrm1 Resource Report Resource Website |
RRID:Addgene_19420 | adhesion regulating molecule 1 | Homo sapiens | Ampicillin | PMID:16906146 | Backbone Marker:Clontech; Backbone Size:5400; Vector Backbone:BacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:45 | 0 |
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