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Species: Phagocata gracilis
Genetic Insert: sfrp-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107662 Copy
Species: Phagocata gracilis
Genetic Insert: slit-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107661 Copy
Species: Dugesia dorotocephala
Genetic Insert: collagen
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107634 Copy
Species: Dugesia dorotocephala
Genetic Insert: innexin-10
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107633 Copy
Species: Danio rerio
Genetic Insert: U6a promoter
Vector Backbone Description: Backbone Size:2651; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107599 Copy
Species: Dugesia dorotocephala
Genetic Insert: laminin
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107632 Copy
Species: Danio rerio
Genetic Insert: polg guide RNA sequence
Vector Backbone Description: Backbone Marker:Qing Deng; Backbone Size:3500; Vector Backbone:p3E-U6a; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107598 Copy
Species: Dugesia dorotocephala
Genetic Insert: porcupine-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107631 Copy
Species: Dugesia dorotocephala
Genetic Insert: opsin
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107630 Copy
Species: Danio rerio
Genetic Insert: sod1
Vector Backbone Description: Backbone Marker:4081; Vector Backbone:pCS2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107594 Copy
Species: Dugesia dorotocephala
Genetic Insert: IFB
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107638 Copy
Species: Dugesia dorotocephala
Genetic Insert: sfrp-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107637 Copy
Species: Phagocata morgani
Genetic Insert: laminin
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107644 Copy
Species: Phagocata morgani
Genetic Insert: PC2
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107641 Copy
Species: Phagocata morgani
Genetic Insert: piwi-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107640 Copy
Species: Phagocata morgani
Genetic Insert: sfrp-1
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107649 Copy
Species: Phagocata morgani
Genetic Insert: ifb
Vector Backbone Description: Backbone Size:2636; Vector Backbone:pPR-T4P; Vector Types:RNAi, In situ hybridization; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_107647 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5922; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29666238
Comments: mCherry - 31% G+C% codon optimised for expression in Bacillus species
Proper citation: RRID:Addgene_107581 Copy
Species: Homo sapiens
Genetic Insert: AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to insert a CAGGS-mTagRFPT-CAAX at the AAVS1 safe harbor locus in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After insertion of the reporter using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_107580 Copy
Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5788; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:29666238
Proper citation: RRID:Addgene_107577 Copy
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