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Species: Synthetic
Genetic Insert: proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt
Vector Backbone Description: Vector Backbone:JAB2120; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951698
Comments: Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190422 Copy
Species: Synthetic
Genetic Insert: AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
Vector Backbone Description: Vector Backbone:Cbh_v5 AAV-ABE N-terminal (Addgene Plasmid #137177); Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38057426
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.01.20.524978v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197509 Copy
Species: Synthetic
Genetic Insert: circular RNA-scaffolded sensing modules
Vector Backbone Description: Backbone Marker:Addgene plasmid #129405; Vector Backbone:pAV U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34431301
Proper citation: RRID:Addgene_185405 Copy
Species: Synthetic
Genetic Insert: LuxA LuxBcp157Venus
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression, E.coli expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34294849
Proper citation: RRID:Addgene_197583 Copy
Species: Synthetic
Genetic Insert: LuxA LuxBVenus
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression, E.coli expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34294849
Proper citation: RRID:Addgene_197582 Copy
Species: Synthetic
Genetic Insert: human codon-optimized Lux-cp157Venus
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34294849
Proper citation: RRID:Addgene_197584 Copy
Species: Synthetic
Genetic Insert: sacB
Vector Backbone Description: Vector Backbone:pCBL101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199735 Copy
Species: Synthetic
Genetic Insert: OsU6.2-sgRNA7 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199733 Copy
Species: Synthetic
Genetic Insert: TaU6-sgRNA8 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199734 Copy
Species: Synthetic
Genetic Insert: ZmU6C3-sgRNA5 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199731 Copy
Species: Synthetic
Genetic Insert: ZmU6C1-sgRNA4 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199730 Copy
Species: Synthetic
Genetic Insert: OsU3-sgRNA2 scaffold
Vector Backbone Description: Vector Backbone:pYPQ132D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199728 Copy
Species: Synthetic
Genetic Insert: TaU3-sgRNA3 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199729 Copy
Species: Synthetic
Genetic Insert: sgRNA scaffold
Vector Backbone Description: Vector Backbone:pYPQ141D; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199726 Copy
Species: Synthetic
Genetic Insert: Multiple Cloning Site, NPTII
Vector Backbone Description: Vector Backbone:pCBL101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199724 Copy
Species: Synthetic
Genetic Insert: NPTII
Vector Backbone Description: Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199722 Copy
Species: Synthetic
Genetic Insert: 35S-RUBY
Vector Backbone Description: Vector Backbone:pCBL101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Comments: Addgene sequencing identified partial duplication of the CaMV 35S enhancer element. Depositing lab has confirmed that the duplication was present in their original stock of the plasmid and does not disrupt RUBY function.
Proper citation: RRID:Addgene_199723 Copy
Species: Synthetic
Genetic Insert: Expression unit for PuroR.T2A.EGFP selectable and reporter markers
Vector Backbone Description: Backbone Size:2850; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36928106
Comments: This target site-containing donor plasmid can be used to insert a transgene(s) at the human CLYBL safe harbor locus when delivered together with Sp-gRNA expression plasmid AY27_pU6.gRNA.CLYBL (Addgene plasmid #199238) or Sa-gRNA expression plasmid AM77_pU6.Sa-gRNA.CLYBL (Addgene plasmid #199237).
Combining this donor with a S. pyogenes Cas9 or a S. aureus Cas9 nuclease allows for HMEJ-mediated gene targeting.
Note: to reduce the chances for off-targeting donor DNA insertions when applying the Sp-gRNA for HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease.
Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) or a S. aureus Cas9 nickase (Addgene plasmid #199251) leads to seamless ITPN-mediated gene targeting.
Proper citation: RRID:Addgene_199227 Copy
Species: Synthetic
Genetic Insert: Expression unit for EGFP reporter
Vector Backbone Description: Backbone Size:2850; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36928106
Comments: This target site-containing donor plasmid can be used to insert a transgene(s) at the human CLYBL safe harbor locus when delivered together with Sp-gRNA expression plasmid AY27_pU6.gRNA.CLYBL (Addgene plasmid #199238) or Sa-gRNA expression plasmid AM77_pU6.Sa-gRNA.CLYBL (Addgene plasmid #199237).
Combining this donor with a S. pyogenes Cas9 or a S. aureus Cas9 nuclease allows for HMEJ-mediated gene targeting.
Note: to reduce the chances for off-targeting donor DNA insertions when applying the Sp-gRNA for HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease.
Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) or a S. aureus Cas9 nickase (Addgene plasmid #199251) leads to seamless ITPN-mediated gene targeting.
Proper citation: RRID:Addgene_199228 Copy
Species: Synthetic
Genetic Insert: Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36928106
Comments: This target site-containing donor plasmid can be used to insert a transgene at the human CCR5 safe harbor locus when delivered together with gRNA expression plasmid AY22_pgRNA.R5.1 (Addgene plasmid #100294) and a SpCas9 nuclease (HMEJ-based gene targeting) or a SpCas9.D10A nickase (ITPN-based gene targeting).
Note: to reduce the chances for off-targeting donor DNA insertions when applying HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease. Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) leads to seamless ITPN-mediated gene targeting.
Proper citation: RRID:Addgene_199223 Copy
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