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Vector Backbone Description: Vector Backbone:pIT5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34190535
Comments: pIT5_KH_VanR is designed to be integrated into the HK022 attachment site, using the HK022 integrase helper plasmid pAH69. This version of plasmid carries VanR repressor, and can be used for inducible gene expression (with Vanillic acid) following integration. The pIT5_KH_VanR need to be propagated in a pir+ strain of E. coli.
Proper citation: RRID:Addgene_173779 Copy
Species: Homo sapiens
Genetic Insert: ArpC4
Vector Backbone Description: Backbone Size:4970; Vector Backbone:pLib; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32917641
Proper citation: RRID:Addgene_173681 Copy
Species: Homo sapiens
Genetic Insert: ArpC5
Vector Backbone Description: Backbone Size:4970; Vector Backbone:pLib; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32917641
Proper citation: RRID:Addgene_173682 Copy
Species: Homo sapiens
Genetic Insert: Arp2
Vector Backbone Description: Backbone Size:5668; Vector Backbone:pBIG2abcd; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32917641
Proper citation: RRID:Addgene_173683 Copy
Species: Homo sapiens
Genetic Insert: SENP1
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17981124
Comments: See author's map for more information.
I193F and T207A mutations are not known to affect function.
Proper citation: RRID:Addgene_17357 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript SKII (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11784061
Proper citation: RRID:Addgene_173686 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Size:0; Vector Backbone:SRa; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9452416
Comments: See author's map for more information.
Proper citation: RRID:Addgene_17359 Copy
Species: Homo sapiens
Genetic Insert: EZH2 RNA-binding mutant
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32632336
Proper citation: RRID:Addgene_173718 Copy
Species: Homo sapiens
Genetic Insert: ArpC2
Vector Backbone Description: Backbone Size:4970; Vector Backbone:pLib; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32917641
Proper citation: RRID:Addgene_173679 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERV1
Vector Backbone Description: Vector Backbone:pDisCoTune; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34405535
Proper citation: RRID:Addgene_173713 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS +; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36223382
Comments: U6 promoter from Anopheles gambiae AGAP013557 drives expression of a classical gRNA template (Jinek et al., 2012) in which a specific gRNA protospacer sequence can be cloned using two BbsI restrictions sites.
Proper citation: RRID:Addgene_173671 Copy
Species: Moloney murine leukemia virus
Genetic Insert: MMLV proviral DNA
Vector Backbone Description: Backbone Size:2000; Vector Backbone:pBR322 modified to include SV40 origin; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10906179
Comments: pNCS contains an infectious copy of M-MuLV proviral DNA and carries a simian virus 40 replication origin to allow high-level expression in 293T cells. DPY-pNCS is similar to the wild-type parent but contains a deletion that removes 5 amino acids (DPPPY) from p12 region.
Proper citation: RRID:Addgene_17365 Copy
Species: Mus musculus
Genetic Insert: Wnt-1HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Proper citation: RRID:Addgene_17367 Copy
Species: Homo sapiens
Genetic Insert: Wnt-2HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Proper citation: RRID:Addgene_17368 Copy
Species: Homo sapiens
Genetic Insert: PAK5 T538N
Vector Backbone Description: Vector Backbone:p-Lenti-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29875996
Proper citation: RRID:Addgene_167181 Copy
Species: Other
Genetic Insert: Blasticidin S deaminase
Vector Backbone Description: Backbone Size:14873; Vector Backbone:lentiCRISPR v2 (Addgene #52961); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34524848
Proper citation: RRID:Addgene_167186 Copy
Species: Homo sapiens
Genetic Insert: PAK5 V593I
Vector Backbone Description: Vector Backbone:plenti-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29875996
Proper citation: RRID:Addgene_167179 Copy
Species: Leptodactylus latrans
Genetic Insert: ATP1A1-R-Q111R-N122D
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5193; Vector Backbone:pFastBac Dual; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33887183
Proper citation: RRID:Addgene_167178 Copy
Species: Leptodactylus latrans
Genetic Insert: ATP1A1-S+Q111R
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5193; Vector Backbone:pFastBac Dual; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33887183
Proper citation: RRID:Addgene_167173 Copy
Species: Leptodactylus latrans
Genetic Insert: ATP1A1-S
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5193; Vector Backbone:pFastBac Dual; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33887183
Proper citation: RRID:Addgene_167172 Copy
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