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Genetic Insert: NLS-LSS-mKate2
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20212155
Comments: There is an 18bp deletion in Addgene's sequencing results relative to the full plasmid sequence (at position 2174-2192bp). This region is immediately after the LSS-mKate2 gene and is not critical for plasmid performance.
There is also a single nucleotide mismatch that results in an F22S change in the linker between the NLS and LSS-mKate2. It should not have any effect on the fusion performance.
Two other mismatches do not alter amino acid sequence.
Proper citation: RRID:Addgene_34586 Copy
Species: Mus musculus
Genetic Insert: Slug
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3xFlag-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21610693
Proper citation: RRID:Addgene_34584 Copy
Genetic Insert: tRNA^Sep
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Tetracycline
Defining Citation: PMID:21868676
Proper citation: RRID:Addgene_34624 Copy
Species: Methanocaldococcuc maripaludis
Genetic Insert: SepRS
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4300; Vector Backbone:pKK223-3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21868676
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
Certain elements of this plasmid are covered under US patent 9,090,928 to Yale University
Proper citation: RRID:Addgene_34623 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Morganstern JP and Land H 1990; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518959
Proper citation: RRID:Addgene_34589 Copy
Species: Mus musculus
Genetic Insert: Snail
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3xFlag-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21610693
Proper citation: RRID:Addgene_34583 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22053050
Proper citation: RRID:Addgene_34611 Copy
Species: Mus musculus
Genetic Insert: ASL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34577 Copy
Species: Mus musculus
Genetic Insert: NFIL-3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21566115
Proper citation: RRID:Addgene_34572 Copy
Species: Mus musculus
Genetic Insert: Arg1 promoter/enhancer -31/-3810
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15187136
Proper citation: RRID:Addgene_34571 Copy
Genetic Insert: synthetic TEAD luciferase reporter
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGL3b; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21654799
Comments: Addgene sequencing detected a 7 nucleotide insertion at position 107 relative to the author's insert sequence. This results in the loss of a MscI restriction site but does not alter the function of the plasmid.
Please click http://www.addgene.org/34615/notes/ for general advice and technical tips on how to monitor YAP/TAZ regulation and activity in cell culture by 8xGTIIC lux.
Proper citation: RRID:Addgene_34615 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pMSCVhygro; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20368440
Proper citation: RRID:Addgene_34565 Copy
Genetic Insert: HRV 3C-GPA-GFP-8HIS
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5329; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20196076
Proper citation: RRID:Addgene_34562 Copy
Species: Homo sapiens
Genetic Insert: SENP3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19015314
Proper citation: RRID:Addgene_34554 Copy
Species: Homo sapiens
Genetic Insert: B23/nucleophosmin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4600; Vector Backbone:pCMV-DsRed-Express; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19015314
Comments: Addgene quality control sequencing found an A123V point mutation, which, according to the depositing lab, should not alter the plasmid's function.
Proper citation: RRID:Addgene_34553 Copy
Species: Homo sapiens
Genetic Insert: SENP5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19015314
Proper citation: RRID:Addgene_34555 Copy
Species: Homo sapiens
Genetic Insert: pLyn-CaRQ
Vector Backbone Description: Backbone Marker:PMID: 12864922 ; Backbone Size:5000; Vector Backbone:pTriEx-3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22195563
Comments: LYN membrane anchor - MGCIKSKGKDSATS
MLCK peptide - KRRWKKNFIAVSAANRFKKI
IQ peptide - GSAITVQRYVRGIQARAYARFL
Proper citation: RRID:Addgene_34559 Copy
Species: Nostoc punctiforme
Genetic Insert: DnaE N-intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3789; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20203672
Proper citation: RRID:Addgene_34549 Copy
Species: Homo sapiens
Genetic Insert: Ple51
Vector Backbone Description: Backbone Size:17451; Vector Backbone:pEMS1302; Vector Types:Pleiades Promoter Project [sic, Pleaides Plieades]; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20807748
Proper citation: RRID:Addgene_29042 Copy
Species: Homo sapiens
Genetic Insert: Ple53
Vector Backbone Description: Backbone Size:16454; Vector Backbone:pEMS1306; Vector Types:Pleiades Promoter Project [sic, Pleaides Plieades]; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20807748
Proper citation: RRID:Addgene_29100 Copy
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